The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Genetic Transformation of the Mycelia of Tremella fuciformis and Changes of Cytotoxicity

흰목이 균사체 형질전환 및 세포독성의 변화

  • Shin, Dong-Il (Department of Biotechnology, Catholic University of Daegu) ;
  • Park, Hee-Sung (Department of Biotechnology, Catholic University of Daegu)
  • 신동일 (대구가톨릭대학교 생명공학과) ;
  • 박희성 (대구가톨릭대학교 생명공학과)
  • Received : 2013.05.29
  • Accepted : 2013.10.07
  • Published : 2013.12.30
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Abstract

Tremella fuciformis, as one of higher basidiomycetes, can asexually reproduce yeast-like conidium (YLC) cells by budding. We have developed an efficient method to introduce pCambia1300 plasmid containing hph gene into YLC cells using Agrobacterium. This was successful only when YLC cells were wounded by NaOH treatment before co-cultivation. In average, 40~50 transformants were produced out of $1.0{\times}10^6$ YLC cells investigated. The T-DNA transfer was confirmed by PCR. Methanolic extracts from transformants demonstrated different levels of toxicity against SKOV-3 cervical cancer cells.

흰목이는 고등 담자균류로서 효모와 유사한 분생포자(yeast-like conidia: YLC)가 출아법에 의하여 번식하는 특징을 지닌다. 본 연구에서는 hph 유전자를 지니는 pCambia 1300 plasmid를 Agrobacterium 이용 YLC 형질전환을 수행하였다. 그 결과 NaOH 처리가 이루어진 YLC 세포를 이용하는 경우에서만 형질전환이 가능하였으며 평균 $1.0{\times}10^6$ YLC 세포들로부터 40-50 형질전환체를 생산할 수 있었다. T-DNA 삽입은 PCR에 의하여 확인되었다. 형질전환체의 메탄올추출액은 암세포주인 SKOV-3에 대한 다양한 수준의 독성이 측정되었다.

Keywords

References

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