The korean journal of orthodontics (대한치과교정학회지)

The Korean Association Of Orthodontists (대한치과교정학회)
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Role of interleukin-6 in orthodontically induced inflammatory root resorption in humans

  • Kunii, Ryuichi (Department of Orthodontics, Nihon University School of Dentistry at Matsudo) ;
  • Yamaguchi, Masaru (Department of Orthodontics, Nihon University School of Dentistry at Matsudo) ;
  • Tanimoto, Yasuhiro (Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo) ;
  • Asano, Masaki (Department of Orthodontics, Nihon University School of Dentistry at Matsudo) ;
  • Yamada, Kunihiko (Department of Orthodontics, Nihon University School of Dentistry at Matsudo) ;
  • Goseki, Takemi (Department of Orthodontics, Nihon University School of Dentistry at Matsudo) ;
  • Kasai, Kazutaka (Department of Orthodontics, Nihon University School of Dentistry at Matsudo)
  • Received : 2013.03.27
  • Accepted : 2013.06.13
  • Published : 2013.12.25
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Abstract

Objective: To determine the interleukin (IL)-6 levels in gingival crevicular fluid (GCF) of patients with severe root resorption after orthodontic treatment and investigate the effects of different static compressive forces (CFs) on IL-6 production by human periodontal ligament (hPDL) cells and the influence of IL-6 on osteoclastic activation from human osteoclastic precursor (hOCP) cells in vitro. Methods: IL-6 levels in GCF samples collected from 20 patients (15 and 5 subjects without and with radiographic evidence of severe root resorption, respectively) who had undergone orthodontic treatment were measured by ELISA. The levels of IL-6 mRNA in hPDL cells and IL-6 protein in conditioned medium after the application of different uniform CFs (0, 1.0, 2.0, or 4.0 $g/cm^2$ for up to 72 h) were measured by real-time PCR and ELISA, respectively. Finally, the influence of IL-6 on mature osteoclasts was investigated by using hOCP cells on dentin slices in a pit-formation assay. Results: Clinically, the IL-6 levels were significantly higher in the resorption group than in the control group. In vitro, IL-6 mRNA expression significantly increased with increasing CF. IL-6 protein secretion also increased in a time- and magnitude-dependent manner. Resorbed areas on dentin slices were significantly greater in the recombinant human IL-6-treated group and group cultured in hPDL cell-conditioned medium with CF application (4.0 $g/cm^2$) than in the group cultured in hPDL cell-conditioned medium without CF application. Conclusions: IL-6 may play an important role in inducing or facilitating orthodontically induced inflammatory root resorption.

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References

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