농업생명과학연구 (Journal of agriculture & life science)

  • 제46권3호
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  • Pages.109-118
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  • 2012
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  • 1598-5504(pISSN)
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  • 2383-8272(eISSN)
경상대학교 농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)
권호 표지

Cloning and Characterization of Carboxylesterase (est2R) Gene from Cow Rumen Metagenomic Library

  • Kang, Tae-Ho (Division of Applied Life Science (BK21 Program), Gyeongsang National University) ;
  • Kim, Min-Keun (Gyeongsangnam-do Agricultural Research and Extension Service) ;
  • Kim, Tae-Yang (Division of Applied Life Science (BK21 Program), Gyeongsang National University) ;
  • Kim, Gi-Hwan (Division of Applied Life Science (BK21 Program), Gyeongsang National University) ;
  • Kim, Jung-Ho (Department of Agricultural Chemistry, Sunchon National University) ;
  • Kim, Hoon (Department of Agricultural Chemistry, Sunchon National University) ;
  • Yun, Han-Dae (Division of Applied Life Science (BK21 Program), Gyeongsang National University, Research Institute of Agriculture and Life Science, Gyeongsang National University)
  • 투고 : 2012.04.30
  • 심사 : 2012.06.26
  • 발행 : 2012.06.30
⟨ 이전 논문 다음 논문 ⟩

초록

The gene encoding an esterase enzyme was cloned from a metagenomic library of cow rumen bacteria. The esterase gene (est2R) was 2,120 bp in length, encoding a protein of 516 amino acid residues with a calculated molecular weight of 57,286 Da. The molecular weight of the enzyme was estimated to be 57,000 Da by SDS-PAGE. Est2R shared 35.6% amino acid identity with esterase (CAH19079) of uncultured prokaryote. The Est2R was most active at $20-40^{\circ}C$, and showed optimum at $30^{\circ}C$ and pH 8.0. The most activity of Est2R for the different chain length of p-nitrophenyl ester group as substrate was p-nitrophenyl acetate. Moreover, the enzyme was found to be most active without organic solvent, followed by 98% active with ethanol, and the enzyme activity was highly affected by the acetonitrile. The enzyme was significantly inhibited by $Zn^{2+}$ but stimulated by $Ca^{2+}$. So, novel esterase gene est2R is likely to obtain from cow rumen metagenome and supposed to use for industrial purpose.

키워드

과제정보

연구 과제 주관 기관 : Rural Development Administration

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