Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
권호 표지

Antioxidant and Neuronal Cell Protective Effects of Aqueous Extracts from Lotus Leaf Tea

  • Jeong, Chang-Ho (Wooyang Frozen Food Co., Ltd.) ;
  • Jeong, Hee-Rok (Dept. of Food Sci. & Techno., Gyeongsang National Univ.(Insti. of Agric. & Life Sci.)) ;
  • Choi, Sung-Gil (Dept. of Food Sci. & Techno., Gyeongsang National Univ.(Insti. of Agric. & Life Sci.)) ;
  • Heo, Ho Jin (Dept. of Food Sci. & Techno., Gyeongsang National Univ.(Insti. of Agric. & Life Sci.))
  • Received : 2011.01.09
  • Accepted : 2012.04.27
  • Published : 2012.04.30
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Abstract

Antioxidant and neuronal cell protective effects of aqueous extract from lotus (Nelumbo nucifera) leaf tea (LLTE) were investigated. The 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging effect, ferric reducing antioxidant power, and malondialdehyde inhibition of LLTE were increased in a dose dependent manner. Intracellular reactive oxygen species accumulation resulting from hydrogen peroxide ($H_2O_2$) treatment was significantly reduced when LLTE were present in the media compared to PC12 cells treated with $H_2O_2$ only. In neuronal cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT), LLTE showed protective effect against $H_2O_2$-induced neurotoxicity. In addition, lactate dehydrogenase release into medium was also inhibited by LLTE (7.13-43.89%). Total phenolics of LLTE were 33.16 mg/g and a quercetin was identified as major phenolics (105.93 mg/100g). Therefore, above these data suggest that LLTE including quercetin may be useful in the natural antioxidant substance, and may reduce the risk of neurodegenerative disease.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea

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