대한기계학회논문집B (Transactions of the Korean Society of Mechanical Engineers B)

  • 제36권11호
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  • Pages.1119-1126
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  • 2012
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  • 1226-4881(pISSN)
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  • 2288-5324(eISSN)
대한기계학회 (The Korean Society of Mechanical Engineers)
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형광 미세입자를 이용한 박테리아 군집의 3차원 형상 분석 및 유동성 생물막의 가시화

Analysis of Three-Dimensional Profile of Bacterial Colony and Visualization of Fluidic Biofilm Using Fluorescent Microbeads

  • 김경훈 (국민대학교 대학원 기계공학과) ;
  • 박은정 (국민대학교 대학원 기계공학과) ;
  • 김중경 (국민대학교 기계시스템공학부)
  • Kim, Kyung-Hoon (Dept. of Mechanical Engineering, Graduate School, Kookmin Univ.) ;
  • Park, Eun-Jung (Dept. of Mechanical Engineering, Graduate School, Kookmin Univ.) ;
  • Kim, Jung-Kyung (School of Mechanical Systems Engineering, Kookmin Univ.)
  • 투고 : 2012.05.18
  • 심사 : 2012.08.24
  • 발행 : 2012.11.01
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초록

세균의 집단 행동은 생물막의 형성에 중요한 역할을 하고 있다. 본 연구에서는 대장균(E. coli) 및 고초균(B. subtilis) 군집에서 형성된 유동성 생물막의 유체역학적 특성을 비교하고자 초기에 아가 플레이트 내에서 층을 이루다가 성장하는 군집 표면 위로 자발적으로 분포되는 200 nm의 형광입자를 가시화 하였다. 대장균 군집에서는 유동하지 않는 200 nm 크기의 형광입자를 이용하여 성장하는 세균 군집의 3차원 형상 프로파일을 측정하였다. 고초균 군집의 경계에서 나타나는 와류 패턴은 고초균이 분비하는 계면활성제 내에서 유동하는 형광입자를 추적하여 가시화하였다. 본 연구는 세균의 생리 기능을 조절하는 새로운 물리적인 요소를 밝혀내고 세균의 증식 및 군집 이동에 영향을 미치는 유동성 생물막의 효과를 파악하는 첫걸음이 될 것이다.

The collective behavior of bacteria plays an important role in biofilm development. In this study, the fluidic properties of biofilms formed in Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis) colonies were compared by visualizing 200-nm fluorescent beads that were initially embedded in an agar plate and distributed spontaneously on the upper surface of the growing colonies. We conducted experiments to measure the three-dimensional profile of the E. coli colony using fluorescent microbeads that did not flow in the colony. Vortical flow patterns near the edge of the B. subtilis colony were observed clearly by tracking the movement of the beads in the biofilm of the colony. The present study should be the first step toward determining the effect of fluidic biofilms on the growth and swarming dynamics of bacteria.

키워드

참고문헌

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피인용 문헌

  1. Visualization of Biosurfactant Film Flow in a Bacillus subtilis Swarm Colony on an Agar Plate vol.16, pp.9, 2015, https://doi.org/10.3390/ijms160920225