KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지
DOI QR코드

DOI QR Code

Isolation of Arthrospira platensis Mutants Producing High Lipid and Phycobiliproteins

지질과 phycobiliproteins 고생산성 Arthrospira platensis 변이주 분리

  • Kim, Young-Hwa (Department of Pharmaceutical Engineering, Silla University) ;
  • Lee, Jae-Hwa (Department of Pharmaceutical Engineering, Silla University)
  • Received : 2012.05.09
  • Accepted : 2012.06.26
  • Published : 2012.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, microalgae Arthrospira platensis (A. platensis) mutants induced by ethyl methane sulfonate (EMS) and further selection for resistance of cerulenin, a potent inhibitor of fatty acid synthase, were characterized. The mutants selected by $2{\mu}M$, $5{\mu}M$ and $10{\mu}M$ of cerulenin were designated EC2, EC5 and EC10, respectively. Under normal growth conditions, the mutants and parental strain exhibited similar growth pattern. The mutants of A. platensis showed enhanced lipid accumulation and phycobiliproteins (phycoerythrin, phycocyanin). The lipid content of mutants EC2 and EC5 was about 4.4 and 4.8-fold higher than wild type. The phycoerythrin and phycocyanin content of mutants EC2 and EC5 was increased about 1.5 and 6.9-fold and 1.4 and 3.8-fold, respectively, compared to the wild type. The chlorophyll and carotenoid content of mutants was slightly increased. The high lipid and pigment contents exhibited by A. platensis mutants would make an excellent candidate for the production of commercially interesting biologically active compounds.

Keywords

References

  1. Kim, Y. M., M. R. Kim, T. H. Kwon, J. M. Ha, and J. H. Lee (2009) Optimum culture conditions for the growth of Spirulina platensis NIES 39. J. Kor. Ind. Eng. Chem. 20: 285-289.
  2. Gantar, M. and Z. Swircev (2008) Microalgae and cyanobacteria: food for thought. J. of Phycol. 44: 260-268. https://doi.org/10.1111/j.1529-8817.2008.00469.x
  3. Gill, I. and R. Valivaty (2001) Polyunsaturated fatty acid: occurrence, biological applications. Trends Biotechnol. 15: 401-409.
  4. Bhat, V. B. and K. M. Madyastha (2000) C-Phycocyanin: a potent peroxyl radical scavenger in vivo and in vitro. Biochem. Biophys. Res. Commun. 275: 20-25. https://doi.org/10.1006/bbrc.2000.3270
  5. Abd, El-Baky H., K. Farouk, and S. Gamal (2007) Production of carotenoids from marine microalgae and its evaluation as safe food colorant and lowering cholesterol agent. American-Eurasian J. of Agricult. Environ. Sci. 2: 792-800.
  6. Carvalho, A. P. and F. X. Malcata (2000) Effect of culture media on production of polyunsaturated fatty acids by Pavlova lutheri. Cryptogram Algol. 21: 59-71. https://doi.org/10.1016/S0181-1568(00)00101-X
  7. Harun, R., M. Singh, G. M. Forde, and M. K. Danquah (2010) Bioprocess engineering of microalgae to produce a variety of consumer products. Renew. Sustain. Energy Reviews 14: 1037-1047. https://doi.org/10.1016/j.rser.2009.11.004
  8. Roman, R. B., J. M. Alvarez-PeZ, F. G. A. Fernandez, and E. M. Grima (2002) Recovery of pure B-phycoerythrin from microalgae Porphyridium cruentum. J. Biotechnol. 93: 73-85. https://doi.org/10.1016/S0168-1656(01)00385-6
  9. Saitoh, S., K. Takahashi, K. Nabeshima, Y. Yamashita, Y. Nakaseko, A. Hirata, and M. Yanagida (1996) Aberrant mitosis in fission yeast mutants defective in fatty acid synthetase and acetyl CoA carboxylase. J. Cell Biol. 134: 949-961. https://doi.org/10.1083/jcb.134.4.949
  10. Heath, R. J., S. W. White, and C. O. Rock (2001) Lipid biosynthesis as a target for antibacterial agents. Prog. Lipid Res. 40: 467-497. https://doi.org/10.1016/S0163-7827(01)00012-1
  11. Carlton, B. C. and B. J. Brown (1981) Gene mutation. In Gerhardt, P. (Ed.) Manual of Methods for General Bacteriology. pp. 85-96. American Society for Microbiology, Washington, DC, USA.
  12. Fischer, R. (1998) Isolation of mutants, a key for the analysis of complex pathways and for strain improvement. In Microbes for Health, Wealth and Sustainable Environment. pp. 739-751. Verma, A., Ed. Malhortra Publishing House, New Delhi, India.
  13. Wang, J., R. Li, D. Lu, S. Ma, Y. Yan, and W. Li (2009) A quick isolation method for mutants with high lipid yield in oleaginous yeast. World J. Microbiol. Biotechnol. 25: 921-925. https://doi.org/10.1007/s11274-009-9960-2
  14. Chaturvedi, R. and Y. Fujita (2006) Isolation of enhanced eicosapentaenoic acid producing mutants of Nannochloropsis oculata ST-6 using ethyl methane sulfonate induced mutagenesis techniques and their characterization at mRNA transcript level. Phycol. Res. 54: 208-219. https://doi.org/10.1111/j.1440-1835.2006.00428.x
  15. Meireles, L. A., A. C. Guedes, and F. X. Malcata (2003) Increase of the yields of eicosapentaenoic and docosahexaenoic acids by the microalga Pavlova lutheri following random mutagenesis. Biotechnol. Bioeng. 81: 50-55. https://doi.org/10.1002/bit.10451
  16. Chen, W., M. Sommerfeld, and Q. Hu (2011) Microwave-assisted nile red method for in vivo quantification of neutral lipids in microalgae. Biores. Technol. 102: 135-141. https://doi.org/10.1016/j.biortech.2010.06.076
  17. Shukla, S. P. and A. K. Kashyap (2003) An assessment of biopotential of three cyanobacterial isolates from antarctic for carotenoid production. Indian J. of Biochem. Biophys. 40: 362-366.
  18. Joo, D. S. and S. Y. Cho (2000) Stability of phycocyanin and spectral characteristic of phycobilins from Spirulina platensis. J. Kor. Fish. Soc. 33: 482-488.
  19. Mata, T. M., A. A. Martins, and N. S. Caetano (2010) Microalgae for biodiesel production and other applications: a review. Renew. Sustain. Energy Reviews. 14: 217-232. https://doi.org/10.1016/j.rser.2009.07.020
  20. Singh, S. C., R. P. Sinha, and D. P. Häder (2002) Role of lipids and fatty acids in stress tolerance in cyanobacteria. Acta. Protozool. 41: 297-308.
  21. Datz, G. and G. Dohler (1981) Light dependent changes in the lipid and fatty acid composition of phycocyanin free photosynthetic lamellae of Synechococcus. Z. Naturforsch. 36: 856-862.
  22. Schoefs, B., N. E. Rmiki, J. Rachadi, and Y. Lemoine (2001) Astaxathin accumulation in Haematococcus requires a cytochrome P450 hydroxylase and an active synthesis of fatty acids. FEBS Letters 500: 125-128. https://doi.org/10.1016/S0014-5793(01)02596-0
  23. Solovchenko, A. E., I. Khozin-Goldberg, S. Didi-Cohen, Z. Cohen, and M. N. Merzlyak (2008) Effects of light and nitrogen starvation on the content and composition of carotenoids of the green microalga Parietochloreis incisa. Russ. J. Plant Physiol. 53: 455-462.

Cited by

  1. Arthrospira platensis Mutants Containing High Lipid Content by Electron Beam Irradiation and Analysis of Its Fatty Acid Composition vol.24, pp.6, 2013, https://doi.org/10.14478/ace.2013.1085
  2. Electron beam-induced mutants of microalgae Arthrospira platensis increased antioxidant activity vol.20, pp.4, 2014, https://doi.org/10.1016/j.jiec.2013.08.039
  3. Isolation of Lipid High-yielding Chlorella vulgaris Mutants by UV Irradiation vol.6, pp.1, 2014, https://doi.org/10.15433/ksmb.2014.6.1.026
  4. Characterization of Arthrospira platensis Cultured in Nano-bubble Hydrogen Water vol.26, pp.4, 2015, https://doi.org/10.14478/ace.2015.1042