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Histomorphometric Analysis of the Spine and Femur in Ovariectomized Rats Using Micro-Computed Tomographic Scan

  • Shin, Yong-Hwan (Department of Neurosurgery, Wooridul Spine Hospital) ;
  • Cho, Dae-Chul (Department of Neurosurgery, Kyungpook National University) ;
  • Yu, Song-Hee (Department of Neurosurgery, Kyungpook National University) ;
  • Kim, Kyoung-Tae (Department of Neurosurgery, Kyungpook National University) ;
  • Cho, Hee-Jung (Department of Anatomy, School of Medicine, Kyungpook National University) ;
  • Sung, Joo-Kyung (Department of Neurosurgery, Kyungpook National University)
  • Received : 2012.01.06
  • Accepted : 2012.07.06
  • Published : 2012.07.28

Abstract

Objective : The purpose of this study was to evaluate the different patterns of bone loss between the lumbar spine and the femur after ovariectomy in rats. Methods : Twenty-four female Sprague-Dawley rats underwent a sham operation (the sham group) or bilateral ovariectomy (the ovariectomized group). Four and eight weeks after operation, six rats from each of the two groups were euthanized. Serum biochemical markers of bone turnover including osteocalcin and alkaline phosphatase (ALP), which are sensitive biochemical markers of bone formation, and the telopeptide fragment of type I collagen C-terminus (CTX), which is a sensitive biochemical marker of bone resorption, were analyzed. Bone histomorphometric parameters of the 4th lumbar vertebrae and femur were determined by micro-computed tomography. Results : Ovariectomized rats were found to have higher osteocalcin, ALP and CTX levels than sham controls. Additionally, 8 weeks after ovariectomy in the OVX group, serum levels of osteocalcin, ALP and CTX were significantly higher than those of 4 weeks after ovariectomy. Bone loss after ovariectomy was more extensive in the 4th lumbar spine compared to the femur. Bone loss in the 4th lumbar spine was mainly caused by trabecular thinning, but in the femur, it was mainly caused by trabecular elimination. Conclusion : The present study demonstrates different patterns of bone loss between the 4th lumbar spine and the femur in ovariectomized rats. Therefore, when considering animal models of osteoporosis, it is important that bone sites should be taken into account.

Keywords

References

  1. Adachi JD, Loannidis G, Berger C, Joseph L, Papaioannou A, Pickard L, et al. : The influence of osteoporotic fractures on health-related quality of life in community-dwelling men and women across Canada. Osteoporos Int 12 : 903-908, 2001. https://doi.org/10.1007/s001980170017
  2. Bauss F, Dempster DW : Effects of ibandronate on bone quality : preclinical studies. Bone 40 : 265-273, 2007 https://doi.org/10.1016/j.bone.2006.08.002
  3. Canpolat S, Tug N, Seyran AD, Kumru S, Yilmaz B : Effects of raloxifene and estradiol on bone turnover parameters in intact and ovariectomized rats. J Physiol Biochem 66 : 23-28, 2010 https://doi.org/10.1007/s13105-010-0008-8
  4. Ferretti M, Bertoni L, Cavani F, Zavatti M, Resca E, Carnevale G, et al. : Influence of ferutinin on bone metabolism in ovariectomized rats. II : role in recovering osteoporosis. J Anat 217 : 48-56, 2010 https://doi.org/10.1111/j.1469-7580.2010.01242.x
  5. French DL, Muir JM, Webber CE : The ovariectomized, mature rat model of postmenopausal osteoporosis : an assessment of the bone sparing effects of curcumin. Phytomedicine 15 : 1069-1078, 2008 https://doi.org/10.1016/j.phymed.2008.06.007
  6. Kim TH, Jung JW, Ha BG, Hong JM, Park EK, Kim HJ, et al. : The effects of luteolin on osteoclast differentiation, function in vitro and ovariectomy-induced bone loss. J Nutr Biochem 22 : 8-15, 2011 https://doi.org/10.1016/j.jnutbio.2009.11.002
  7. Kleerekoper M, Villanueva AR, Stanciu J, Rao DS, Parfitt AM : The role of three-dimensional trabecular microstructure in the pathogenesis of vertebral compression fractures. Calcif Tissue Int 37 : 594-597, 1985 https://doi.org/10.1007/BF02554913
  8. Lei Z, Xiaoying Z, Xingguo L : Ovariectomy-associated changes in bone mineral density and bone marrow haematopoiesis in rats. Int J Exp Pathol 90 : 512-519, 2009 https://doi.org/10.1111/j.1365-2613.2009.00661.x
  9. Meli R, Pacilio M, Raso GM, Esposito E, Coppola A, Nasti A, et al. : Estrogen and raloxifene modulate leptin and its receptor in hypothalamus and adipose tissue from ovariectomized rats. Endocrinology 145 : 3115-3121, 2004 https://doi.org/10.1210/en.2004-0129
  10. Miller SC, Bowman BM, Jee WS : Available animal models of osteopenia--small and large. Bone 17 : 117S-123S, 1995
  11. Mosekilde L : Assessing bone quality--animal models in preclinical osteoporosis research. Bone 17 : 343S-352S, 1995
  12. Omi N, Ezawa I : The effect of ovariectomy on bone metabolism in rats. Bone 17 : 163S-168S, 1995
  13. Palumbo C, Ferretti M, Bertoni L, Cavani F, Resca E, Casolari B, et al. : Influence of ferutinin on bone metabolism in ovariectomized rats. I : role in preventing osteoporosis. J Bone Miner Metab 27 : 538-545, 2009 https://doi.org/10.1007/s00774-009-0070-x
  14. Park SB, Lee YJ, Chung CK : Bone mineral density changes after ovariectomy in rats as an osteopenic model : stepwise description of double dorso-lateral approach. J Korean Neurosurg Soc 48 : 309-312, 2010 https://doi.org/10.3340/jkns.2010.48.4.309
  15. Sheng ZF, Dai RC, Wu XP, Fang LN, Fan HJ, Liao EY : Regionally specific compensation for bone loss in the tibial trabeculae of estrogen-deficient rats. Acta Radiol 48 : 531-539, 2007 https://doi.org/10.1080/02841850701283761
  16. Shiraishi A, Miyabe S, Nakano T, Umakoshi Y, Ito M, Mihara M : The combination therapy with alfacalcidol and risedronate improves the mechanical property in lumbar spine by affecting the material properties in an ovariectomized rat model of osteoporosis. BMC Musculoskelet Disord 10 : 66, 2000
  17. Szulc P, Delmas PD : Biochemical markers of bone turnover : potential use in the investigation and management of postmenopausal osteoporosis. Osteoporos Int 19 : 1683-1704, 2008 https://doi.org/10.1007/s00198-008-0660-9
  18. Thompson DD, Simmons HA, Pirie CM, Ke HZ : FDA Guidelines and animal models for osteoporosis. Bone 17 : 125S-133S, 1995
  19. Turner AS : Animal models of osteoporosis--necessity and limitations. Eur Cell Mater 1 : 66-81, 2001 https://doi.org/10.22203/eCM.v001a08

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