Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지
DOI QR코드

DOI QR Code

Detection of Ocular Toxoplasma gondii Infection in Chronic Irregular Recurrent Uveitis by PCR

  • Lee, Sang-Eun (Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention) ;
  • Hong, Sung-Hee (Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention) ;
  • Lee, Seong-Ho (R & D Center, Lumieye Genetics Co. Ltd.) ;
  • Jeong, Young-Il (Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention) ;
  • Lim, Su-Jin (The Retina Center, Nune Eye Hospital) ;
  • Kwon, Oh-Woong (The Retina Center, Nune Eye Hospital) ;
  • Kim, Sun-Hyun (The Retina Center, Nune Eye Hospital) ;
  • You, Young-Sung (The Retina Center, Nune Eye Hospital) ;
  • Cho, Shin-Hyeong (Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention) ;
  • Lee, Won-Ja (Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention)
  • Received : 2012.02.07
  • Accepted : 2012.04.14
  • Published : 2012.09.15
Download PDF
⟨ Previous Next ⟩

Abstract

Toxoplasma gondii is a zoonotic parasite resulting in human infections and one of the infectious pathogens leading to uveitis and retinochoroiditis. The present study was performed to assess T. gondii infection in 20 ocular patients with chronic irregular recurrent uveitis (20 aqueous humor and 20 peripheral blood samples) using PCR. All samples were analyzed by nested PCR targeting a specific B1 gene of T. gondii. The PCR-positive rate was 25% (5/20), including 5% (1) in blood samples, 25% (5) in aqueous humor samples, and 5% (1) in both sample types. A molecular screening test for T. gondii infection in ocular patients with common clinical findings of an unclear retinal margin and an inflammatory membrane over the retina, as seen by fundus examination, may be helpful for early diagnosis and treatment.

Keywords

References

  1. Bossi P, Bricaire F. Severe acute disseminated toxoplasmosis. Lancet 2004; 364: 579.
  2. Keino H, Nakashima C, Watanabe T, Taki W, Hayakawa R, Sugitani A, Okada AA. Frequency and clinical features of intraocular inflammation in Tokyo. Clin Experiment Ophthalmol 2009; 37: 595-601. https://doi.org/10.1111/j.1442-9071.2009.02102.x
  3. Rhthova A, de Boer JH, Ten Dam-van Loon NH, Postma G, de Visser L, Zuurveen SJ, Schuller M, Weersink AJ, van Loon AM, de Groot-Mijnes JD. Usefulness of aqueous humor analysis for the diagnosis of posterior uveitis. Ophthalmology 2008; 115: 306-311. https://doi.org/10.1016/j.ophtha.2007.05.014
  4. Smith JR, Cunningham ET Jr. Atypical presentations of ocular toxoplasmosis. Curr Opin Ophthalmol 2002; 13: 387-392. https://doi.org/10.1097/00055735-200212000-00008
  5. De-la-Torre A, Lopez-Castillo CA, Rueda JC, Mantilla RD, Gomez-Marin JE, Anaya JM. Clinical patterns of uveitis in two ophthalmology centres in Bogota, Colombia. Clin Exp Ophthalmol 2009; 37: 458-466. https://doi.org/10.1111/j.1442-9071.2009.02082.x
  6. Sirirungsi W, Pathanapitoon K, Kongyai N, Weersink A, de Gerro-Mijnes JD, Leechanachai P, Ausayakhun S, Rothova A. Infectious uveitis in Thailand: Serologic analyses and clinical features. Ocul Immunol Inflamm 2009; 17: 17-22. https://doi.org/10.1080/09273940802553816
  7. London NJ, Hovakimyan A, Cubillan LD, Siverio CD Jr, Cunningham ET Jr. Prevalence, clinical characteristics, and causes of vision loss in patients with ocular toxoplasmosis. Eur J Ophthalmol 2011; 21: 811-819. https://doi.org/10.5301/EJO.2011.6403
  8. Kong SM, Lee TS, Kim CW. 3 Cases of ocular toxoplasmosis. J Korean Ophthalmol Soc 1975; 16: 141-145.
  9. Yoo HY, Cho BC. 2 Cases of ocular toxplasmosis. J Korean Ophthalmol Soc 1979; 20: 231-237.
  10. Shyn KH, Kim KY, Park SC. A clinical experience of ocular toxoplasmosis, treated with acetyl spiramycin. J Korean Ophthalmol Soc 1979; 20: 427-431.
  11. Kim MH, Choi YK, Park YK, Nam HW. A toxoplasmic uveitis case of a 60-year-old male in Korea. Korean J Parasitol 2000; 38: 29-31. https://doi.org/10.3347/kjp.2000.38.1.29
  12. Chai JY, Lin A, Shin EH, Oh MD, Han ET, Nam HW, Lee SH. Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea. Korean J Parasitol 2003; 41: 147-154. https://doi.org/10.3347/kjp.2003.41.3.147
  13. Park YH, Han JH, Nam HW. Clinical features of ocular toxoplasmosis in Korean patients. Korean J Parasitol 2011; 49: 167-171. https://doi.org/10.3347/kjp.2011.49.2.167
  14. Hurtado A, Aduriz G, Moreno B, Barandika J, Garcia-Perez AL. Single tube nested PCR for detection of Toxoplasma gondii in fetal tissues from naturally aborted ewes. Vet Parasitol 2001; 102: 17-27. https://doi.org/10.1016/S0304-4017(01)00526-X
  15. Fardeau C, Romand S, Rao NA, Cassoux N, Bettembourg O, Thulliez P, Lehoang P. Diagnosis of toxoplasmic retinochoroiditis with atypical clinical features. Am J Ophthalmol 2002; 134: 196-203. https://doi.org/10.1016/S0002-9394(02)01500-3
  16. Figueroa MS, Bou G, Marti-Belda P, Lopez-Velez R, Guerrero A. Diagnostic value of polymerase chain reaction in blood and aqueous humor in immunocompetent patients with ocular toxoplasmosis. Retina 2000; 20: 614-619. https://doi.org/10.1097/00006982-200011000-00005
  17. Villard O, Filisetti D, Roch-Deries F, Garweg J, Flament J, Candolfi E. Comparison of enzyme-linked immunosorbent assay, Immunoblotting, and PCR for diagnosis of Toxoplasmic chorioretinitis. J Clin Microbiol 2003; 41: 3537-3541. https://doi.org/10.1128/JCM.41.8.3537-3541.2003
  18. Payeur G, Bijon JC, Pagano N, Kien T, Candolfi E, Penner MF. Diagnosis of ocular toxoplasmosis by the ELISA method applied to the determination of immunoglobulins of the aqueous humor. J Fr Ophtalmol 1988; 11: 75-79.
  19. Bidgoli S, Koch P, Caspers L. Toxoplasmic chorioretinitis: positive PCR on vitreous with negative serology for Toxoplasma gondii. J Fr Ophtalmol 2011; 34: 384.e1-e5. https://doi.org/10.1016/j.jfo.2011.01.015
  20. Silveira C, Vallochi AL, Rodrigues da Silva UR, Muccioli C, Holland GN, Nussenblatt RB, Belfort R, Rizzo LV. Toxoplasma gondii in the peripheral blood of patients with acute and chronic toxoplasmosis. Br J Ophthalmol 2011; 95: 396-400. https://doi.org/10.1136/bjo.2008.148205
  21. Bastien P. Molecular diagnosis of toxoplasmosis. Trans R Soc Trop Med Hyg 2002; 96: S205-S215. https://doi.org/10.1016/S0035-9203(02)90078-7
  22. Accorinti M, Bruscolini A, Pirraglia MP, Liverani M, Caggiano C. Toxoplasmic retinochoroiditis in an Italian referral center. Eur J Ophthalmol 2009; 19: 824-830. https://doi.org/10.1177/112067210901900522

Cited by

  1. Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea vol.50, pp.4, 2012, https://doi.org/10.3347/kjp.2012.50.4.287
  2. Prevalence and Genetic Characterization of Toxoplasma gondii in House Sparrows (Passer domesticus) in Lanzhou, China vol.51, pp.3, 2012, https://doi.org/10.3347/kjp.2013.51.3.363
  3. IgG Avidity Antibodies against Toxoplasma gondii in High Risk Females of Reproductive Age Group in India vol.52, pp.5, 2012, https://doi.org/10.3347/kjp.2014.52.5.487