Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지
DOI QR코드

DOI QR Code

Infection of Clinostomum complanatum (Treamdoda: Digenea) metacercaria in cultured southern top mouthed minnow Pseudorasbora parva

참붕어, Pseudorasbora parva의 인두흡충, Clinostomum complanatum 피낭유충 감염증

  • Jung, Eun-Bin (Department of Aquatic life Medicine, Kunsan National University) ;
  • Kim, Dong-Wan (Dongkyung Microbial Technology) ;
  • Kim, Jin-Do (Pathology Division, National Fisheries Research and Development Institute) ;
  • Park, Sung-Woo (Department of Aquatic life Medicine, Kunsan National University)
  • Received : 2011.12.22
  • Accepted : 2012.07.24
  • Published : 2012.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Many metacercariae in the musculature of southern top mouthed minnow (Pseudorasbora parva) cultured at a farm in Chonbuk Province. The excysted metacercariae looked yellowish white and were $4.02{\pm}1.80$ mm (1.66-5.81) in length and $1.27{\pm}0.27$ mm (1.01-1.64) in width for acetocarmin-stained specimens. The oral sucker measuring $0.18{\pm}0.05$ mm (0.11-0.24) in length and $0.23{\pm}0.07$ mm (0.15-0.33) in width, was located at the anterior end of the body. The ventral sucker situated at the fourth third of the body were measured $0.47{\pm}0.08$ mm (0.35-0.53) in length and $0.55{\pm}0.09$ mm (0.43-0.63) in width. The tongue-shaped metacercariae were identified as Clinostomum complanatum on the basis of morphological characteristics. The metacercaria was also confirmed to be C. complanatum using PCR reaction with C. complanatum-specific primer sets, Trem 18S F and Ccom670 R. No histopathological changes in the infected fish were observed except necrosis and exfoliation of epidermis in the skin and atrophy of muscle fibers.

노지 양식장에서 비단잉어와 함께 사육중인 참붕어의 미병부, 체측근, 복부 및 아가미 뚜껑 내부에 황백색의 cyst내의 metacercaria를 탈낭시켜 형태학적 및 분자생물학적으로 조사한 결과 Clinostomum complanatum으로 동정되었다. 병리조직학적으로는 상피세포의 박리와 괴사 및 cyst주변의 근섬유의 위축이외에 다른 조직의 변화는 발견되지 않았다.

Keywords

References

  1. Aohagi, Y. and Shibahara, T.: Clinostomum complanatum infection in Crassius spp. collected from some ponds and rivers in Tottori and Shimane prefectures, Japan. Jpn. J. Parasitol., 43: 129-135, 1994.
  2. Aohagi, Y., Shibahara, T., Machida, N., Yamaga, Y., Kagota, K., and Hayashi, T.: Natural infections of Clinostomum complanatum (Tremadoda: Clinostomatidase) in wild herons and egrets, Tottori Prefecture, Japan. J. Wildlife. Dis., 28: 470-471, 1992. https://doi.org/10.7589/0090-3558-28.3.470
  3. Chung, D-1., Kong, H-E., and Joo, C-Y.: Radix auricularia coreana: natural snail host of Clinostomum complanatum in Korea. Kor. J. Parasitiol., 36: 1-6, 1998. https://doi.org/10.3347/kjp.1998.36.1.1
  4. Chung, D. I., Kong, H. H., and Moon, C. H.: Demonstration of the second intermediate hosts of Clinostomum complanatum in Korea. Kor. J. Parasitol., 33: 305-312, 1995B. https://doi.org/10.3347/kjp.1995.33.4.305
  5. Chung, D. I., Moon, C-H., Kong, H-H, Choi, D-W., and Lim, D-K.: The first human case of Clinostomum complanatum (Trematoda: Clinostomidae) infection in Korea. Kor. J. Parasitol., 33: 219-223, 1995A. https://doi.org/10.3347/kjp.1995.33.3.219
  6. Dzikowski, R., Levy, M., Poore, M. F., Flower, J. R., and Paperna, I: Clinostomum complanatum and Clinostomum marginatum (Rudolphi, 1819) (Digenea: Clinostomidae) are separated species based on differences in ribosomal DNA. J. Parasitol., 90: 413-414, 2004. https://doi.org/10.1645/GE-159R
  7. Kagei, N., Yanohara, Y., Uchikawa, R., and Sato, S.: On the yellow grubs, metacercariae of Clinostomum complanatum (Rudolphi, 1814), found in the cultured loach. Jpn. J. Parasitiol., 33: 59-62, 1984.
  8. Kakantan, A. M. N. and Arfin, M.: In vitro excysation of metacercariae of Clinostomum complanatum (Rudolphi, 1819) (Digenea: Clinostomatidae). Acta Zoologica, 69: 163-167, 1988. https://doi.org/10.1111/j.1463-6395.1988.tb00913.x
  9. Kalantan, A. M. N., Arfin, M. and Nizami, W. A.: Histochemical nature and origin of the metacercarial cyst of Clinostomum complanatum (Trematoda: Digenea). Trans. Am. Microscop. Soc., 105: 290-295, 1986. https://doi.org/10.2307/3226301
  10. Kim, Y.-G. and Nagasawa, K.: Infection of Clinostomum complanatum (Rudolphi, 1814) (Trematoda: Digenea) metacercaria in goldfish (Crassius auratus) cultured in Korea. J. Fish Pathol., 9: 1-9, 1996.
  11. Levy, M. G., Flowers, J. R., Poore, M. F., Khoo, L., Pote, L. M., Muller, J. R., Paperna, I., Dzikowski, R., and Litaker, R. W.: Morphologic, pathologic, and genetic investigation of Bolbophorus species affecting cultured channel catfish in the Mississipi Delta. J. Aquat. Anim. Health. 14: 235-246, 2002. https://doi.org/10.1577/1548-8667(2002)014<0235:MPAGIO>2.0.CO;2
  12. Lo, C-F., Huber, F., Kou G-H., and Lo, C-J.: Studies of Clinostomum complanatum (RUD, 1819). Fish Pathol., 15: 219-227, 1981. https://doi.org/10.3147/jsfp.15.219
  13. Malek, M., and Mobedi, I.: Occurrence of Clinostomum complanatum (Rudolphi, 1819) (Digenea: Clinostomatidae) in Capoeta capoeta gracilis (Osteichthys: Cyprinidase) from Shiround River, Iran. Iranian J. Publ. Health, 30: 95-98, 2001.
  14. Park, C-W., Kim, J-S., Joo, H-S., and Kim, J.: A human case of Clinostomum complanatum infection in Korea. Kor. J. Parasitiol., 47: 401-404, 2009. https://doi.org/10.3347/kjp.2009.47.4.401
  15. Sohn, W-M.: Fish-borne zoonotic trematode metacercariae in the Republic of Korea. Kor. J. Parasitol., 47, Supplement: S103-S113, 2009. https://doi.org/10.3347/kjp.2009.47.S.S103
  16. 김익수, 박종영: 원색도감.한국의 자연시리즈 18. 한국의 민물고기. 교학사, 2002.
  17. 김지형, 김경연, 오태엽, 장환, 조성준, Gomez, D. K., Choresca, C. H., 박세창: 어름치(Hemibarbus mylodon) 에서 Clinostomum complantum 감염증. J. Vet. Clin., 25: 307-309, 2008.
  18. 이재구: 간흡충의 제 2중간숙주에 관한 실험적 연구. III. 담수어류 표피의 곤봉상세포와 간흡충 피낭유 충의 감염도에 관하여. 기생충학잡지, 12(2): 101-110, 1974.
  19. 전세규: 간흡충의 감염경로에 관한 실험적 연구. 제 3편각종 어류 체표면 점액물질의 간흡충에 대한 살충효과. 기생충학잡지, 2(3): 12-22, 1964.

Cited by

  1. 국내 중소하천에서 피라미 (Zacco platypus)의 복강 기생충 감염특성 vol.49, pp.3, 2012, https://doi.org/10.11614/ksl.2016.49.3.215