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Lactoferrin Stimulates Mouse Macrophage to Express BAFF via Smad3 Pathway

  • Chang, Heyn-Keyung (Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University) ;
  • Jin, Bo-Ra (Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University) ;
  • Jang, Young-Saeng (Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University) ;
  • Kim, Woan-Sub (Division of Animal Life and Environmental Science, College of Agriculture and Life Science, Hankyong National University) ;
  • Kim, Pyeung-Hyeun (Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University)
  • Received : 2012.04.18
  • Accepted : 2012.05.18
  • Published : 2012.06.30
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Abstract

B cell-activating factor belonging to the TNF family (BAFF) is primarily expressed by macrophages and stimulates B cell proliferation, differentiation, survival, and Ig production. In this study, we explored the effect of lactoferrin (LF) on BAFF expression by murine macrophages. We determined the level of BAFF expression at the transcriptional and protein levels using RT-PCR and ELISA, respectively. LF markedly enhanced BAFF expression in mouse macrophages at both the transcriptional and protein levels. Overexpression of Smad3/4 further increased LF-induced BAFF transcription while DN-Smad3 abolished the LF-induced BAFF expression. These results demonstrate that LF can enhance BAFF expression through Smad3/4 pathway.

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References

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