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The Analysis of Vitamin C Concentration in Organs of $Gulo^{-/-}$ Mice Upon Vitamin C Withdrawal

  • Kim, Hye-Min (Department of Anatomy, Medical Research Center, Seoul National University) ;
  • Bae, Se-Yeon (Department of Anatomy, Medical Research Center, Seoul National University) ;
  • Yu, Yeon-Sil (Department of Anatomy, Medical Research Center, Seoul National University) ;
  • Kim, Ye-Jin (Department of Anatomy, Medical Research Center, Seoul National University) ;
  • Kim, Hang-Rae (Department of Anatomy, Medical Research Center, Seoul National University) ;
  • Hwang, Young-Il (Department of Anatomy, Medical Research Center, Seoul National University) ;
  • Kang, Jae-Seung (Department of Anatomy, Medical Research Center, Seoul National University) ;
  • Lee, Wang-Jae (Department of Anatomy, Medical Research Center, Seoul National University)
  • Received : 2011.12.23
  • Accepted : 2012.01.30
  • Published : 2012.02.29
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Abstract

Background: Vitamin C is an essential nutrient for maintaining human life. Vitamin C insufficiency in the plasma is closely related with the development of scurvy. However, in vivo kinetics of vitamin C regarding its storage and consumption is still largely unknown. Methods: We used $Gulo^{-/-}$ mice, which cannot synthesize vitamin C like human. Vitamin C level in plasma and organs from $Gulo^{-/-}$ mice was examined, and it compared with the level of wild-type mice during 5 weeks. Results: The significant weight loss of $Gulo^{-/-}$ mice was shown at 3 weeks after vitamin C withdrawal. However, there was no differences between wild-type and vitamin C-supplemented $Gulo^{-/-}$ mice (3.3 g/L in drinking water). The concentration of vitamin C in plasma and organs was significantly decreased at 1 week after vitamin C withdrawal. Vitamin C is preferentially deposited in adrenal gland, lymph node, lung, and brain. There were no significant changes in the numbers and CD4/CD8 ratio of splenocytes in $Gulo^{-/-}$ mice with vitamin C withdrawal for 4 weeks. And the architecture of spleen in $Gulo^{-/-}$ mice was disrupted at 5 weeks after vitamin C withdrawal. Conclusion: The vitamin C level of $Gulo^{-/-}$ mice was considerably decreased from 1 week after vitamin C withdrawal. Vitamin C is preferentially stored in some organs such as brain, adrenal gland and lung.

Keywords

References

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