생명과학회지 (Journal of Life Science)

  • 제22권9호
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  • Pages.1214-1223
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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진범(Aconitum pseudolaeve var erectum Nakai)의 생리활성과 진범 향이 뇌파에 미치는 영향

Physiological Activity of Aconitum pseudolaeve var erectum Nakai and Its Effect on Perfume on an Electroencephalogram (EEG)

  • 이진영 (호서대학교 한방화장품과학과) ;
  • 최연희 ((주)제이에스향료 연구소 조향팀) ;
  • 이새미 ((주)제이에스향료 연구소 조향팀) ;
  • 이수연 (호서대학교 한방화장품과학과) ;
  • 전혜지 (호서대학교 한방화장품과학과)
  • Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Choi, Yeon-Hee (JS Perfumery Co., Ltd., Fragrance R&D center) ;
  • Lee, Sae-Mi (JS Perfumery Co., Ltd., Fragrance R&D center) ;
  • Lee, Soo-Yeon (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Jun, Hye-Ji (Department of Herbal Cosmetic Science, Hoseo University)
  • 투고 : 2012.07.03
  • 심사 : 2012.09.05
  • 발행 : 2012.09.30
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초록

본 연구는 진범의 in-vitro 생리활성과 진범 향이 신경생리학적으로 뇌파에 미치는 영향을 비교 분석하였다. 그 결과 진범 향 및 재조합 향 흡입 시 흡입 전에 비해 뇌파지표 absolute alpha power spectrum (AA)와 absolute theta power spectrum (AT)가 유의미하게 감소하였으며(p<0.05), 진범과 진범 재조합 향 흡입 시 출현한 파형의 유발 부위가 각각 조금씩 다른 양상을 나타내기는 하였지만, 동일하게 alpha와 theta파가 나타났으며, 증가율 역시 향 흡입 시 감소하는 동일 양상을 나타내어 뇌에 각성효과를 일으킨 것으로 판단된다. 진범의 생리활성 검증 결과 수렴효과를 제외한 DPPH, collagenase, nitric oxide 저해활성 검증에서 진범 에탄올 추출물의 활성이 열수 추출물에 비해 우수하였으며, 특히 collagenase저해활성에서 진범 에탄올 추출물 500 ppm에서 80%의 저해활성을 나타내었으며, 또한 nitric oxide 저해활성에서 진범 열수 및 에탄올 추출물 100 ppm에서 50% 이상의 NO저해활성을 나타내었다. 이러한 결과로부터 진범은 화장품 및 향료 개발의 천연소재로 사용이 가능할 것으로 판단된다.

This study analyzed the in vitro physiological activity of Aconitum pseudolaeve var erectum Nakai (AP) and its effect on perfume on an electroencephalogram (EEG). The results indicated that the absolute alpha power spectrum (AA) and the absolute theta power spectrum (AT) decreased significantly during more than before the inhalation of the AP perfume and its reconstruction perfume. Although there were a little different pattern in the induced part of the wave generated by the inhalation of the AP perfume and its reconstruction perfume, alpha- and theta- were shown equally. In addition, there was the same pattern in which the rate of increase reduced. The results suggest that the perfume of the AP and its reconstruction perfume have a stimulating effect on the brain. In terms of the physiological activity of AP, the activity of AP ethanol extract was significantly higher than that of water extract in DPPH, collagenase, and nitric oxide, except for an astringent effect. The AP ethanol extract was about 80% at 500 ppm in collagenase inhibition activity. In addition, the AP water and ethanol extracts were 50% at 100 ppm in the NO inhibition activity. Based on these results, we conclude that this natural substance could be used in cosmetics and in the development of perfumes.

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참고문헌

  1. Acuna, B. D., Eliassen, J. C., Donoghue, J. P. and Sanes, J. N. 2002. Frontal and parietal lobe activation during transitive inference in humans. Cereb. Cortex 12, 1312-1321. https://doi.org/10.1093/cercor/12.12.1312
  2. Anderson, R. and Mohme-Lundholm, E. 1969. Studies on the relaxing actions mediated by stimulation of adrenergic alpha- and beta- receptors in taenia coli of the rabbit and guinea pig. Acta. Physiol. Scand. 77, 372-384. https://doi.org/10.1111/j.1748-1716.1969.tb04582.x
  3. Artru, P. and Lledo, G. 2005. Traitement adjuvant du cancer du colon a l'asco : L' oxaliplatine accroit sa suprematie. Acta endoscopica, 35, 710. https://doi.org/10.1007/BF03003935
  4. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1120.
  5. Carmichael, J., DeGraff, W. G., Gazdar, A. F., Minna, J. D. and Mitchell, J. B. 1987. Evaluation of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47, 936-942.
  6. Choi, M. E., Jeon, B. K., Kim, D. S., Mun, Y. J. and Woo, W. H. 2009. A study on application for beauty food of mixture of Korean red ginseng and Fagopyrum esculentum: Anti-oxidative effect and collagenase inhibitory activity. Kor. J. Oriental Med. Prescription 17, 153-162.
  7. Hong, S. K., Jeong, D. M., Kim, K. Y. and Hwang, E. H. 2010. The composition of the root of lxeris dentata var. albiflora Nakai, and cell viability and DPPH radical scavenging activities of its extract. Kor. J. Nutr. 43, 105-113. https://doi.org/10.4163/kjn.2010.43.2.105
  8. Huh, M., Lawson, A. E. and Kwon, Y. J. 1997. The role of prefrontal lobes in scientific reasoning. J. Kor. Assoc. Sci. Edu. 17, 525-540.
  9. Jeong, Y. J., Choi, S. Y., An, C. S., Jeon, Y. H., Park, D. K. and Lim, B. O. 2009. Comparative effect on anti-inflammatory activity of the Phellinus linteus and Phellinus linteus grown in germinated brown rice extracts in murine macrophage Raw 264.7 cells. Kor. J. Med. Crop. Sci. 17, 97-101.
  10. Jung, H. J. 2009. The effects of augmentative and alternative communication on SMR-wave and $\beta$-wave of students with communication. MS Thesis, Daegu university, Daegu, Korea.
  11. Kim, D. K., Kwak, J. H., Song, K. W., Kwon, H. C., Zee, O. P. and Lee, K. R. 1996. Phytochemical constituents from Aconitum pseudolaeve var erectum. Kor. J. Pharmacogn. 27, 75-79.
  12. Kim, J. R. 2009. Scientific analysis of QEEG activation for teaching reading using digital text to elementary school students. MS Thesis, KyungSung University , Busan, Korea.
  13. Kim, Y. H., Cho, W. A., Cheon, S. J., Jang, M. J., Sung, J. Y., Jung, S. H., Choi, H. J., Kim, D. I., Kim, J. O., Lee, C. E., An, B. J. and Lee, J. T. 2007. Study on anti-oxidant and cosmeceutical activities of Isatis tinctoria L. Kor. J. Herbol. 22, 85-91.
  14. Kim, Y. J. and Chang, N. G. 2000. Development of a brain-cycle learning model based on the rhythmicity in brain functions and its test by brain wave's measure. Kor. J. Biol. Edu. 28, 396-407.
  15. Klem, G. H., Luders, H. O., Jasper, H. H. and Elger, C. 1958. The ten-twenty electrode system of the international federation. Electroencephalogr. Clin. Neurophysiol. Suppl. 52, 3-6.
  16. Klimesch, W. 1995. Memory processes described as brain oscillations in the EEG-alpha and theta bands. Psychology 11, 134-143.
  17. Kropotov, J. D. 2009. Quantitative EEG, event-related potentials and neurotherapy. pp. 59-76. Academic Press, San Diego, California.
  18. Lee, H. S. and Chung, B. S. 1989. Studies on Korean Aconitum Species(V). On the chemical constituents of aconitum pseudolaeve var erectum. Kor. J. Pharmaecogn. 20, 6-9.
  19. Lee, I. H. 1997. Psychophysiology, pp.15-16. Hakjisa Press, Seoul, Korea.
  20. Lee, J. T., Jeong, Y. S. and An, B. J. 2002. Physiological activity of Salicornia herbacea and its application for cosmetic materials. Kor. J. Herbol. 17, 51-60.
  21. Lee, S. K., Kim, Y. W., Shin, D. Y., Lim, M. K., Lee, J. H., Song, J. M. and Kim, L. H. 2007. The changes of electroencephalography according to emotional stimulus in Sasangin. J. Sasang Constitution Med. 19, 113-126.
  22. Lee, T. W., Kim, S. N., Jee, U. K. and Hwang, S. J. 2004. Anti-wrinkle effect of pressure sensitive adhesive hydrogel patches containing Ulmi cortex extract. J. Kor. Pharm. Sci. 34, 193-199. https://doi.org/10.4333/KPS.2004.34.3.193
  23. Marklund, S. and Marklund, G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47, 469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  24. Menon, V., Rivera, S. M., White, C. D., Eliez, S., Glover, G. H. and Reiss, A. L. 2000. Functional optimization of arithmetic processing in perfect performers. Cognitive Brain Res. 9, 343-345. https://doi.org/10.1016/S0926-6410(00)00010-0
  25. Moncada, S., Palmer, R. M. and Higgs, E. A. 1991. Nitric oxide: physiology, patho- physiology, and pharmacology. Pharmacol. Rev. 43, 109-142.
  26. Nathan, C. 1992. Nitric oxide as a secretory product of mammalian cells. Faseb. J. 6, 3051-3064.
  27. Park, C. W. 1998. Brain: Structure of learning and memory. pp. 1-76, Seoul National University Press, Seoul, Korea.
  28. Park, S. J., Oh, S. B., Lee, E. J. and Seo, H. J. 2012. Psychophysiological evaluation for naturally flavored cream products. Kor. J. Sci. Emotion Sensibility 15, 9-16.
  29. Seo, I. A. 2010. Studies on cosmeceutical of Phaseolus radiates. MD. Thesis, Daegu Haany University, Gyeongsan, Korea.
  30. Seong, K. E. 2008. The effect of mathematical activity on a learner's brain activity. MS. Thesis, Ajou University, Suwon, Korea.
  31. Wunsch, E. and Heindrich, H. G. 1963. Zur quantitativen bestimmung der collagenase. Hoppe-Seyler's. Physiol. Chem. 333, 149-151. https://doi.org/10.1515/bchm2.1963.333.1.149

피인용 문헌

  1. The Complete Chloroplast Genome Sequences of Aconitum pseudolaeve and Aconitum longecassidatum, and Development of Molecular Markers for Distinguishing Species in the Aconitum Subgenus Lycoctonum vol.22, pp.12, 2017, https://doi.org/10.3390/molecules22112012