Journal of Sasang Constitutional Medicine (사상체질의학회지)

The Society Of Sasang Constitutional Medicine (사상체질의학회)
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Effects of Korean Herbal Bathing Extracts Composition on Th2 Cytokine Production in MC/9 Mast Cells

한방 입욕제 조성물이 MC/9 mast cell에서의 Th2 cytokine 발현에 미치는 영향

  • Jang, Moon-Hee (Dept. of Sasang Constitutional Medicine, College Oriental Medicine, Daejeon Univ.) ;
  • Choi, Jae-Song (Dept. of Sasang Constitutional Medicine, College Oriental Medicine, Daejeon Univ.) ;
  • Choi, Ae-Ryun (Dept. of Sasang Constitutional Medicine, College Oriental Medicine, Daegu Haany Univ.) ;
  • Ahn, Taek-Won (Dept. of Sasang Constitutional Medicine, College Oriental Medicine, Daejeon Univ.)
  • 장문희 (대전대학교 한의과대학 사상체질의학과) ;
  • 최재송 (대전대학교 한의과대학 사상체질의학과) ;
  • 최애련 (대구한의대학교 한의과대학 사상체질의학과) ;
  • 안택원 (대전대학교 한의과대학 사상체질의학과)
  • Received : 2012.08.07
  • Accepted : 2012.09.17
  • Published : 2012.09.30
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Abstract

Objectives The purpose of this study is to investigate the effects of korean herbal bathing extracts composition 1 and composition 2 on Th2 cytokine production in MC/9 mast cells. Methods The effects of composition 1, 2 was analyzed by ELISA and Real-time PCR in MC/9 mast cells. Levels of IL-5, IL-13 were measured using enzyme-linked immunosorbent assays(ELISA). mRNA levels of IL-4, IL-5, IL-6, IL-13 were analyzed with Real-time PCR. Results Composition 1, 2 inhibited the IL-5, IL-13 production significantly(p<.001) in comparison to PI-control group at concentration of 100 ${\mu}g/mL$, 200 ${\mu}g/mL$. Composition 1, 2 inhibited the IL-4, IL-5, IL-13 mRNA expression significantly in comparison to PI-control group at concentration of 100 ${\mu}g/mL$, 200 ${\mu}g/mL$. Composition 1 inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 200 ${\mu}g/ml$. Composition 2 inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 100 ${\mu}g/mL$, 200 ${\mu}g/mL$. Conclusions These results indicate that composition 1, 2 has the effect of decreasing the Th2 cytokine production in the MC/9 mast cell.

Keywords

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