Natural Product Sciences

  • 제18권3호
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  • Pages.177-182
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  • 2012
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  • 1226-3907(pISSN)
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  • 2288-9027(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

Neuroprotective Effects of Cambodian Plant Extracts on Glutamate-induced Cytotoxicity in HT22 Cells

  • Keo, Samell (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University) ;
  • Lee, Dong-Sung (Hanbang Body-Fluid Research Center, Wonkwang University) ;
  • Li, Bin (Hanbang Body-Fluid Research Center, Wonkwang University) ;
  • Choi, Hyun-Gyu (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University) ;
  • Kim, Kyoung-Su (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University) ;
  • Ko, Won-Min (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University) ;
  • Oh, Hyun-Cheol (College of Pharmacy, Wonkwang University) ;
  • Kim, Youn-Chul (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
  • 투고 : 2012.05.25
  • 심사 : 2012.07.26
  • 발행 : 2012.09.30
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초록

Oxidative stress potentially induces neurotoxicity which is believed to underlie several major age-related diseases of the central nervous system. This study sought to identify the cytoprotective effects of sixty-nine Cambodian plants against glutamate-induced cell death. Cultured HT22 cells were applied as an in vitro model, and neurotoxicity was induced in these neuronal cells by exposure to a determined concentration of glutamate. Sixty-nine plant sources, as Cambodia's indigenous species, were purchased from O'reusey Market, Phnom Penh, and extracted with ethanol. These extracts were screened for cytoprotective effects against glutamate-triggered neurotoxicity in HT22 cells at concentrations of 100 and 300 ${\mu}g/ml$. Of these, eight ethanol extracts, bark of Anacardium occidentale, bark and sapwood of Bauhinia pulla, flowers of Borassus flabellifer, stems and leaves of Coix lacryma-jobi, bark and sapwood of Diospyros nitida, sapwood of Dipterocarpus obtusifolius, stems of Oryza rufipogon, and fruits of Phyllanthus emblica, showed significant cytoprotective effects against glutamate-induced cell damage and degeneration in HT22 cells.

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