Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibitory Effect of Artemisinic Acid Isolated from Artemisia Annua L on the MDC in HaCaT Keratinocytes

HaCaT 각질형성세포에서 개똥쑥(Artemisia annua L) 유래 성분인 Artemisinic acid의 Macrophage-derived Chemokine 억제 효과

  • Kang, Gyeoung-Jin (Department of Pharmacology, School of Medicine, Jeju National University) ;
  • Kang, Na-Jin (Department of Pharmacology, School of Medicine, Jeju National University) ;
  • Han, Sang-Chul (Department of Pharmacology, School of Medicine, Jeju National University) ;
  • Koo, Dong-Hwan (Department of Pharmacology, School of Medicine, Jeju National University) ;
  • Kim, Young-Soo (Biospectrum Life Science Institute) ;
  • Lee, Jin-Hyuck (Biospectrum Life Science Institute) ;
  • Kim, Sang-Chul (Biospectrum Life Science Institute) ;
  • Park, Deok-Hoon (Biospectrum Life Science Institute) ;
  • Lee, Jong-Sung (Department of Dermatological Health Management, Eul-Ji University) ;
  • Kang, Hee-Kyung (Department of Pharmacology, School of Medicine, Jeju National University) ;
  • Yoo, Eun-Sook (Department of Pharmacology, School of Medicine, Jeju National University)
  • 강경진 (제주대학교 의학전문대학원 약리학교실) ;
  • 강나진 (제주대학교 의학전문대학원 약리학교실) ;
  • 한상철 (제주대학교 의학전문대학원 약리학교실) ;
  • 구동환 (제주대학교 의학전문대학원 약리학교실) ;
  • 김영수 (바이오스펙트럼 생명과학 연구소) ;
  • 이진혁 (바이오스펙트럼 생명과학 연구소) ;
  • 김상철 (바이오스펙트럼 생명과학 연구소) ;
  • 박덕훈 (바이오스펙트럼 생명과학 연구소) ;
  • 이종성 (을지대학교 피부관리학과) ;
  • 강희경 (제주대학교 의학전문대학원 약리학교실) ;
  • 유은숙 (제주대학교 의학전문대학원 약리학교실)
  • Received : 2012.08.10
  • Accepted : 2012.09.18
  • Published : 2012.09.30
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Abstract

In the present study, we investigated anti-inflammatory activity of artemisinic acid in HaCaT cells and RAW264.7 cells. Artemisinic acid showed inhibitory activity on macrophage-derived chemokines (MDC) expression, a factor related with atopic dermatitis (AD), in interferon (IFN)-${\gamma}$ and tumor necrosis factor (TNF)-${\alpha}$-stimulated HaCaT cells. In the study on action mechanism, pretreated artemisinic acid reduced the phosphorylation of STAT1 and p38 and the degradation of $I{\kappa}B$ by IFN-${\gamma}$ and TNF-${\alpha}$ stimulations. However, artemisinic acid didn't show the inhibitory activity on LPS-induced inflammatory mediators (NO, $PGE_2$, IL-6) in RAW264.7 cell. These results indicate that artemisinic acid inhibits IFN-${\gamma}$ and TNF-${\alpha}$-induced MDC expression through inhibition of signal factors, STAT1, NF-${\kappa}B$, and p38, in HaCaT keratinocytes.

Keywords

References

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