Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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A Thirteen Week Repeated Oral Dose Toxicity Test and A Four Week Recovery Test of ACM(Added Chongmyung-tang) in Sprague-Dawley Rats

ACM의 Rat를 이용한 13주 반복 경구투여 독성시험 및 4주 회복시험

  • Park, Dae-Myung (Dept. of Neuropsychiatry, College of Oriental Medicine, Daejeon University) ;
  • Lee, Sang-Ryong (Dept. of Neuropsychiatry, College of Oriental Medicine, Daejeon University) ;
  • Lim, Jong-Soon (Dept. of Life Science and Preventative Medicine College of Oriental Medicine, DaeJeon University) ;
  • Kim, Seung-Hyung (Institute of Traditional Medicine and Bioscience, DaeJeon University) ;
  • Jung, In-Chul (Dept. of Neuropsychiatry, College of Oriental Medicine, Daejeon University)
  • 박대명 (대전대학교 한의과대학 신경정신과학교실) ;
  • 이상룡 (대전대학교 한의과대학 신경정신과학교실) ;
  • 임종순 (대전대학교 한의과대학 생명예방과학교실) ;
  • 김승형 (대전대학교 동서생명과학연구원) ;
  • 정인철 (대전대학교 한의과대학 신경정신과학교실)
  • Received : 2012.08.17
  • Accepted : 2012.09.13
  • Published : 2012.09.30
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Abstract

Objectives : To provide information on the safety of ACM, we carried out a 13-week repeated oral dose toxicity and a 4-week recovery test of ACM in Sprague-Dawley rats. Methods : Female and male rats were treated with ACM with oral doses of 800, 2000, and 5000 mg/kg. The ACM was administered for 13 weeks. Mortality, clinical signs, body weight changes, food consumption, ophthalmologic findings, urinalysis, hematological and biochemical parameters, gross findings, organ weights and histological markers were monitored during the study period. Moreover, the rats were monitored for 4 extra weeks to determine recovery time after the study period. Results : We found no mortality and no abnormalities in clinical signs, body weight, food consumption, ophthalmologic findings, urinalysis, hematological and biochemical parameters, gross findings, organ weights and histological markers in any of the rats tested. Conclusions : The no-observed adverse effects level (NOAEL) was considered as over 5000 mg/kg for male and female rats.

Keywords

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