Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Buddleja officinalis on Cadmium-induced Cytototoxicty in HK-2 Cells

HK-2 세포에서 카드뮴 세포독성에 대한 밀몽화(密蒙花)의 효과

  • Ju, Sung-Min (Department of Pathology, College of Korean Medicine, Wonkwang University) ;
  • Kang, Min-Soo (Department of Pathology, College of Korean Medicine, Wonkwang University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Korean Medicine, Wonkwang University)
  • 주성민 (원광대학교 한의과대학 병리학교실) ;
  • 강민수 (원광대학교 한의과대학 병리학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실)
  • Received : 2012.07.23
  • Accepted : 2012.08.16
  • Published : 2012.08.25
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Abstract

Cadmium is an important occupational and environmental pollutant that damages various organs, especially renal proximal tubular cells. We examined the effect of aqueous extract of Buddleja officinalis (ABO) on cadmium chloride ($CdCl_2$)-induced cytotoxicity in HK-2 human renal proximal tubular cells. HK-2 cells were preincubated with ABO (50, 100, 200 and 400 ${\mu}g/ml$) for 3 hr and then treated with 10 ${\mu}M$ $CdCl_2$ for 24 hr. The effect of ABO on $CdCl_2$-induced cytotoxicity in HK-2 cells was investigated by using MTT assay, morphological observation, flow cytometric analysis and Western blot. The results of the MTT assay and morphological observation indicated that $CdCl_2$-induced cytotoxicity was prevented by pretreatment with ABO. In flow cytometric analysis, ABO reduced sub-G1 peak (apoptotic peak) in $CdCl_2$-treated cells. $CdCl_2$-induced procaspase-3 proteolysis and PARP cleavage reduced by pretreatment with ABO. These results suggest that ABO effectively inhibited $CdCl_2$-induced cytotoxicity in HK-2 cells.

Keywords

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