Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Antioxidant and Antimicrobial Activities of Xanthium sibiricum

창이자의 항산화 활성 및 항균효과

  • Shin, Sun-Woo (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Jeong-Ho (Department of Natural Health Management, Songho College) ;
  • Soo, Bang-Keuk (Department of Chinese Medicine Resource, Chonbuk National University)
  • 신선우 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 이정호 (송호대학교 동의건강복지과) ;
  • 방극수 (전북대학교 환경생명자원대학 한약자원학과)
  • Received : 2010.07.23
  • Accepted : 2012.06.04
  • Published : 2012.08.29
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Abstract

Xanthium sibiricum using extracts antioxidant activity and antimicrobial activity were analyzed and the following results were obtained. Antioxidant activity using DPPH as a VLC column fractionation experiments XS-5 (water fraction of Xanthium sibiricum) concentration in the 500 ${\mu}g/ml$ to 87.53 percent in the highest antioxidant activity was found, VLC fractionation in XS-3-2 (eluted with chloroform/methanol 7~10%) concentration of 500 ${\mu}g/ml$, showed the antioxidant activity of 78.72%. The antioxidant activity measured by PCL XS-1 (n-hexane fraction of Xanthium sibiricum) XS-5 (water fraction of Xanthium sibiricum) and 13.32 nmol open column XS-3-2 (eluted with chloroform/methanol 7~10%) showed antioxidant activity with 14.34 nmol. The antimicrobial activity against Candida albicans XS-2 (methlene chloride fraction fo Xanthium sibiricum) is the concentration of 500 ${\mu}g$/disc 1 mm of the clean zone was formed. XS-3 (ethanol fraction fo Xanthium sibiricum) in the antioxidant activity using DPPH and PCL had higher antibacterial activity was low.

창이자 추출물을 이용하여 항산화활성 및 항균활성을 측정한 결과 다음과 같은 결론을 얻었다. DPPH를 이용한 항산화 활성 실험결과 VLC column로 분획한 XS-5에서 처리 농도 500 ${\mu}g/ml$에서의 87.53%로 가장 우수한 항산화 활성이 나타났으며, VLC로 분획한 XS-3-2의 처리농도 500 ${\mu}g/ml$에서 78.72%의 항산화 활성이 나타났다. PCL법으로 측정한 항산화 활성은 XS-1에서 13.32 nmol과 open column XS-3-2에서 14.34 nmol으로 항산화력을 나타났다. Candida albicans에 대한 항균활성은 XS-2의 처리농도가 500 ${\mu}g$/disc에서는 1 mm의 clean zone이 형성되었다. XS-3에서 DPPH와 PCL를 이용한 항산화 활성은 높았지만 항균활성은 낮게 나타났다. 창이자 추출물은 항산화 활성과 항균 활성은 상호 상관관계가 없는 것으로 판단된다.

Keywords

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