Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Phylogenetic Analysis of Korean Native Goats Based on the Mitochondrial Cytochrome b Gene

mtDNA Cytochrome b 유전자에 기초한 한국재래염소의 계통유전학적 분석

  • 김재환 (농촌진흥청 국립축산과학원) ;
  • 변미정 (농촌진흥청 국립축산과학원) ;
  • 고응규 (농촌진흥청 국립축산과학원) ;
  • 김성우 (농촌진흥청 국립축산과학원) ;
  • 김상우 (농촌진흥청 국립축산과학원) ;
  • 도윤정 (농촌진흥청 국립축산과학원) ;
  • 김명직 (농촌진흥청 국립축산과학원) ;
  • 윤세형 (농촌진흥청 국립축산과학원) ;
  • 최성복 (농촌진흥청 국립축산과학원)
  • Received : 2012.04.10
  • Accepted : 2012.05.24
  • Published : 2012.08.31
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Abstract

The goal of this study was to verify the phylogenetic status of the Korean native goats (KNG). We determined the complete sequence of the mitochondrial cytochrome b gene in 48 goats among four populations. We also analyzed genetic variability within goats, and a phylogenetic analysis was performed by comparison with other country's goats. Three nucleotide substitutions were detected, and two of these were missense mutations that occurred due to a substitution of amino acid. Four haplotypes were defined from KNG. Three of these haplotypes were only found in the Chinese goat. However, the other haplotype was KNG-specific. In the phylogenetic analysis, four clades (A~D) were classified among domestic goats, and the KNG was classified into clade 1 that estimated as lineage A based on the D-loop sequence. Each haplotype from the KNG was clustered closely with that of the Chinese goat. The results of haplotype distribution and phylogenetic location suggest that strong gene flow occurred from China to the Korean Peninsula.

한국재래염소의 계통유전학적 위치를 확인하기 위해서 한국재래염소 4개 집단 48두를 공시한 후 mitochondrial DNA (mtDNA) 내부의 cytochrome b 유전자의 전체서열을 분석하였다. 또한 이 서열들을 이용하여 한국재래염소의 유전적 다양성을 확인하였고, 다른 나라의 여러 염소품종들과의 계통유전학적 분석을 수행하였다. 한국재래염소 cytochrome b 유전자 서열을 토대로 3개의 염기변이가 동정되었으며, 그 중 2개는 아미노산 치환을 일으키는 missense 변이로 확인되었다. 또한 4개의 haplotype으로 분류되었는데, 이 중 3개는 중국 재래염소 품종에서도 나타났으나 다른 나라의 품종에서는 확인되지 않았다. 계통유전학적 분석 결과 모든 재래흑염소는 4개의 clade를 형성하였으나, 5개의 야생염소와는 독립적인 그룹을 형성하였다. 한국재래염소는 mtDNA D-loop에 분류되는 여러 모계혈통 중 모계혈통-A로 추정되는 clade 1에 포함되었다. 한국재래염소에서 보여진 각각의 haplotype은 중국 재래염소품종들과 상대적으로 가까운 유전적 유연관계를 보였다. 기존 연구결과와 본 연구의 분석결과를 종합해보면 과거에 일부 중국 재래 염소품종이 한반도로 유입되어 한국재래염소의 기원 및 가축화에 영향을 주었을 것으로 사료된다.

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