대한기계학회논문집B (Transactions of the Korean Society of Mechanical Engineers B)

  • 제36권9호
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  • Pages.901-905
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  • 2012
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  • 1226-4881(pISSN)
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  • 2288-5324(eISSN)
대한기계학회 (The Korean Society of Mechanical Engineers)
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다수의 전기장 분포가 생성되는 단일 미세유로를 이용한 폐암세포 전기천공 및 활성도 분석칩

Electroporation and Viability Monitoring Chip for Lung Cancer Cells in Single Channel with Multiple Electric Field Zones

  • 투고 : 2012.01.06
  • 심사 : 2012.07.18
  • 발행 : 2012.09.01
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초록

본 논문에서는 다수의 전기장 분포가 생성되는 단일 미세유로를 이용한 폐암세포 전기천공 및 활성도 분석칩을 제안하였다. 종래의 세포 전기천공 분석칩은 다수의 전기장 분포를 형성하기 위해 다수의 전극패턴 또는 다수의 미세유로를 필요로 하여 구조가 복잡하였다. 반면, 제안된 세포 전기천공 및 활성도 분석칩은 한 쌍의 전극 사이에서 계단 형상으로 폭이 변화하는 단일 미세유로를 이용하여 다수의 전기장 분포를 형성함으로써 간단한 구조로 세포 전기천공 및 활성도를 분석할 수 있다. 제안된 세포 전기천공 및 활성도 분석칩은 0.3kV/cm에서 0.5kV/cm까지 5단계의 전기장이 발생되도록 설계하였다. A549와 H23의 두 종류의 비소세포 폐암세포주를 이용한 성능실험 결과, 활성을 유지하면서 전기천공된 세포의 비율이 0.4kV/cm의 전기장에서 각각 $26.6{\pm}0.7%$ 및 $51.4{\pm}3.0%$로 가장 높은 값을 보였다. 제안된 세포 전기천공 및 활성도 분석칩은 세포의 형질주입 연구를 위한 집적화된 세포칩으로 응용될 수 있다.

We present an electroporation and viability monitoring chip for lung cancer cells in a single channel with multiple electric field zones. Previous electroporation chips utilized multiple microchannels or electrodes to form multiple electric fields, thus resulting in complex structures. However, the present chip can generate multiple electric fields in a single stepwise microchannel between a pair of electrodes, thus achieving the analysis of both cell electroporation and viability with a simple structure. We demonstrate that the electric field of 0.4 kV/cm results in a maximum percentage of $51.4{\pm}3.0%$ and $26.6{\pm}0.7%$ of viable and electroporated human lung cancer cells, H23 and A549, respectively. The present chip has potential for use in integrated cell chips for transfection studies.

키워드

참고문헌

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