YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Evaluation of Oxidative Stress and Antioxidant Enzyme Expression in Human Hepatocarcinoma SK-Hep-1 Cells Treated with Stearic Acid

인간 간암 세포주인 SK-Hep-1에서 Stearic Acid에 의한 산화적 스트레스 및 항산화효소의 발현변화 평가

  • Oh, Jung-Min (College of Pharmacy Chungnam National University) ;
  • Lee, Ji-Yoon (College of Pharmacy Chungnam National University) ;
  • Lee, Gwan-Ho (College of Pharmacy Chungnam National University) ;
  • Kim, Bong-Hee (College of Pharmacy Chungnam National University) ;
  • Kim, Sang-Kyum (College of Pharmacy Chungnam National University)
  • Received : 2011.12.30
  • Accepted : 2012.02.01
  • Published : 2012.02.29
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Abstract

Nonalcoholic steatohepatitis(NASH) is serious metabolic disease related to fatty acid. According to "two hit theory", fatty acid-induced oxidative stress is important factor to progress nonalcoholic steatohepatitis from steatosis. In this study, we evaluated stearic acid induced oxidative stress in human hepatocarcinoma SK-Hep-1 cell. Cell viability, reactive oxygen species (ROS) production, glutathione (GSH), malondialdehyde and expression of antioxidant enzymes were determined at various time-points and concentrations of stearic acid. At 0.2 mM, non-toxic concentration, of stearic acid, production of ROS was significantly increased at 24 hours and the level of GSH was significantly decreased. Expression of superoxide dismutase-1 and 2 was slightly increased in 0.2 mM stearic acid at 24 hours. These results represent that the non-toxic concentration of stearic acid resulted in oxidative stress, suggesting that stearic acid may play a critical role in development of steatohepatitis.

Keywords

References

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