Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Suppression of Transglutaminase-2 is Involved in Anti-Inflammatory Actions of Glucosamine in 12-O-Tetradecanoylphorbol-13-Acetate-Induced Skin Inflammation

  • Park, Mi-Kyung (College of Pharmacy, Dongguk University) ;
  • Cho, Sun-A (R & D Center, AmorePacific Corporation) ;
  • Lee, Hye-Ja (College of Pharmacy, Dongguk University) ;
  • Lee, Eun-Ji (College of Pharmacy, Dongguk University) ;
  • Kang, June-Hee (College of Pharmacy, Dongguk University) ;
  • Kim, You-Lee (College of Pharmacy, Dongguk University) ;
  • Kim, Hyun-Ji (College of Pharmacy, Dongguk University) ;
  • Oh, Seung-Hyun (College of Pharmacy, Gachon University of Medicine and Science) ;
  • Choi, Chang-Sun (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University) ;
  • Lee, Ho (College of Pharmacy, Gachon University of Medicine and Science) ;
  • Kim, Soo-Youl (National Cancer Center) ;
  • Lee, Chang-Hoon (College of Pharmacy, Dongguk University)
  • Received : 2012.05.29
  • Accepted : 2012.07.10
  • Published : 2012.07.31
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Abstract

Glucosamine (GS) is well known for the treatment of inflammation. However, the mechanism and efficacy of GS for skin inflammation are unclear. The aim of this study was to evaluate the effects and mechanism of GS in the mouse 12-O-tetradecanoyl 13-acetate (TPA)-induced ear edema model. TPA-induced ear edema was evoked in ICR or transglutaminase 2 (Tgase-2) (-/-) mice. GS was administered orally (10-100 mg/kg) or topically (0.5-2.0 w/v %) prior to TPA treatment. Orally administered GS at 10 mg/kg showed a 76 or 57% reduction in ear weight or myeloperoxidase, respectively, and a decreased expression of cyclooxygenase-2 (COX-2), NF-${\kappa}B$ and Tgase-2 in TPA-induced ear edema by western blot and immunohistochemistry. Role of Tgase-2 in TPA ear edema is examined using Tgase-2 (-/-) mice and TPA did not induce COX-2 expression in ear of Tgase-2 (-/-) mice. These observations suggested that Tgase-2 is involved in TPA-induced COX-2 expression in the inflamed ear of mice and antiinflammatory effects of glucosamine is mediated through suppression of Tgase-2 in TPA ear edema.

Keywords

References

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