The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Evaluation of endocrine disrupting effect of the herbicide Alachlor on Japanese Medaka using short term reproduction assay

단기번식독성시험법을 이용한 제초제 Alachlor의 송사리 내분비계 영향 조사

  • 이제봉 (농촌진흥정 농업과학기술원) ;
  • 박연기 (농촌진흥정 농업과학기술원) ;
  • 최영웅 (농촌진흥정 농업과학기술원) ;
  • 김병석 (농촌진흥정 농업과학기술원) ;
  • 권혜영 (농촌진흥정 농업과학기술원) ;
  • 진용덕 (농촌진흥정 농업과학기술원) ;
  • 임건재 (농촌진흥정 농업과학기술원) ;
  • 강규영 (경상대학교 환경생명화학과)
  • Received : 2012.04.17
  • Accepted : 2012.05.11
  • Published : 2012.06.30
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Abstract

Acute toxicity, water resolvability and short term reproduction test on Japanese medaka (Oriyzias latipes) for evaluating alachlor susceptibility to endocrine system were studied. Alachlor is known for suspected endocrine distruptors. As the results of tests, $LC_{50}$ (Median lethal concentration) was determined as 2.36 (1.994~2.805) mg/L, and test water replaced at 7 day intervals as its water resolvability was less than 20% in 7 days. The short term reproduction tests on Japanese medaka (Oriyzias latipes) were performed with a solvent control group, a treated group (alachlor concentrations of 0.02, 0.04, 0.11, 0.27, 0.68 ppm) and a positive control group (17 ${\beta}$ estradiol, 0.01, 0.1, 0.5 ppb). The number of spawning and embryo rates were declined in a alachlor-dose dependent manner, and the number of unfertilized eggs rates were in contrast increased depending on the concentrations. Further study should be needed to confirm whether the adverse effects may be effected by the concentrations. Additionally, alachlor was evaluated as a non-vitellogenin by the result of a test of significance of the vitellogenin content test for determination of the effect of estrogen among the endocrine disruptors.

내분비계 장애 추정물질로 알려진 alachlor의 어류 생체내 호르몬에 미치는 영향을 검색하고자 송사리를 이용한 급성독성, 수중 분해성 및 단기번식독성시험을 수행하였다. 시험결과 96시간 $LC_{50}$은 2.37(1.994~2.805) mg/L 이었으며, 수중 분해성이 15일에 초기 처리농도에 비해 20% 정도 분해되었으므로 단기번식독성시험의 시험용수 교체는 매 7일로 하였다. 송사리를 이용한 21일 단기번식시험의 노출농도는 용매대조군, alachlor(0.02, 0.04, 0.11, 0.27, 0.68ppm)처리군, 양성 대조군($17{\beta}$ estradiol, 0.01, 0.1, 0.5ppb)을 두어 수행한 결과 산란 수는 대조군에 비해 최고투여 농도에서 47.9% 감소하였고, 유정란 율은 47.8% 감소하였다. 기형율도 부분적으로 농도 의존성 경향이 관찰되었다. 또한 내분비계 장애성 중 에스트로겐 영향을 검색할 목적으로 어체내 vitellogenin 농도 변화를 조사한 결과 유의성이 나타나지 않았으므로 alachlor는 vitellogenin성 에스트로겐은 아닌 것으로 판단되었다.

Keywords

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