The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Enhancing effect of Canavalia gladiata DC semen on the hematopoietic expansion and function of stem cells

도두(刀豆)의 조혈증진 작용에 관한 연구

  • Kim, Seung-Hyung (Institute of Traditional Medicine and Bioscience, Daejeon University) ;
  • Kim, Kun-Hoae (Central Research Laboratories of Aekyung industrial Co.Ltd.) ;
  • Chi, Gyeong-Yup (Central Research Laboratories of Aekyung industrial Co.Ltd.) ;
  • Cho, In-Sik (Central Research Laboratories of Aekyung industrial Co.Ltd.) ;
  • Kim, Han-Young (Central Research Laboratories of Aekyung industrial Co.Ltd.) ;
  • Lee, Young-Cheol (Department of Herbology, College of Oriental Medicine, Sangji University)
  • Received : 2012.06.19
  • Accepted : 2012.06.28
  • Published : 2012.07.30
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Abstract

Objective : Canavalia gladiata DC semen (CGS) have been used to improve hematopoietic activity. In the current study, we investigated whether CGS regulate hemato-potentiating function using hematopoietic stem cells (HSCs) as a testing system. Methods : HSCs isolated from femur in mice with leukopenia and thrombocytopenia induced induced by CTX. Then, Real-time PCR was performed to measure the mRNA expression and hematopoietic related gene (EPO, IL-3, SCF, c-kit, GM-CSF), the phoaphorylation of GATA-1 and STAT-5a/b were observed by ELISA method, and the number of granulocyte erythrocyte monocyte macrophage colony-forming units (CFU-GEMM) and erythroid burst forming units (BFU-E), semisolid clonogenic assay was performed. Result : When HSCs were treated with CGS, the expression of hematopoietic related genes (EPO, IL-3, SCF, c-kit, and GM-CSF) were significantly increased at the levels of mRNA as well as production in HSCs. Additionally, CGS enhanced phosphorylation of STAT-1 and signal transducer and activator of transcription-5a/b (STAT-5a/b) in HSCs. Furthermore, CGS significantly enhanced the growth rate of granulocyte erythrocyte monocyte macrophage colony-forming units (CFU-GEMM) and erythroid burst forming units (BFU-E) in vitro. Conclusion : These result suggest that CGS has hematopoietic enhancement via hematopoietic cytokine-mediated GATA-1/STAT-5a/b pathway.

Keywords

References

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