Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Whitening Effect of Hizikia fusiformis Ethanol Extract and Its Fractions

톳(Hizikia fusiformis) 에탄올 추출물 및 분획물의 미백활성

  • Jeon, Myong-Je (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Kim, Mi-Hyang (Department of Food and Nutrition, Silla University) ;
  • Jang, Hye-Ji (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Lee, Seung-Woo (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Kim, Jae-Hoon (Haeim Pharmetic Co., Marine-Bio Center) ;
  • Kim, Hyung-Suk (Haeim Pharmetic Co., Marine-Bio Center) ;
  • Lee, Sang-Hyeon (Department of Bioscience and Biotechnology, Graduate School, Silla University)
  • 전명제 (신라대학교 일반대학원 생명공학과) ;
  • 김미향 (신라대학교 식품영양학과) ;
  • 장혜지 (신라대학교 일반대학원 생명공학과) ;
  • 이승우 (신라대학교 일반대학원 생명공학과) ;
  • 김재훈 ((주)해림파메틱) ;
  • 김형석 ((주)해림파메틱) ;
  • 이상현 (신라대학교 일반대학원 생명공학과)
  • Received : 2012.02.14
  • Accepted : 2012.07.20
  • Published : 2012.07.30
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Abstract

Melanin synthesis is catalyzed by tyrosinase. To investigate the whitening effect of Hizikia fusiformis, fractions from ethanol extract of H. fusiformis were prepared by a systematic fractionation procedure with solvents such as methanol, hexane, butanol, and $H_2O$. The ethanol extract and its fractions were then subjected to evaluate the inhibitory effects on the tyrosinase activity and melanin synthesis in murine B16F10 melanoma cells. The ethanol extract and aqueous fraction exhibited a whitening effect with no cytotoxicity. The ethanol extract showed the highest whitening effect among the samples. The inhibitory effect of $100{\mu}g/ml$ of ethanol extract was higher than that of $10{\mu}g/ml$ of arbutin, but it was lower than that of $10{\mu}g/ml$ of kojic acid. Furthermore, the inhibitory effects of $100{\mu}g/ml$ of methanol, hexane, butanol, and aqueous fractions were similar to those of $10{\mu}g/ml$ of arbutin. The antioxidant activities were examined by comparing the results with that of ascorbic acid as a positive control. The ethanol extract and aqueous fraction showed relatively higher DPPH radical-scavenging activities compared with the other samples. Furthermore, $500{\mu}g/ml$ of ethanol extract and aqueous fraction diminished LPS-induced iNOS expression to 82 and 80%, respectively. These results suggest that ethanol extract and aqueous fraction of H. fusiformis could be used as cosmetic ingredients for whitening and skin protection effects.

멜라닌 생합성은 tyrosinase의 효소적 산화반응에 의해 일어난다. 톳(Hizikia fusiformis)의 미백효과를 검증하기 위하여 메탄올, 헥산, 부탄올 및 물을 용매로 이용한 분획과정을 통해 톳 에탄올 추출물로부터 분획물을 제조하였다. 에탄올 추출물과 분획물들을 대상으로 B16F10 흑색종 세포에서 tyrosinase 활성 저해효과 및 멜라닌 생합성 억제효과를 평가하였다. 생쥐의 에탄올 추출물 및 수층 분획물은 미백효과를 나타냈지만, 세포독성은 나타내지 않았다. 에탄올 추출물은 시료들 중에서 가장 높은 미백활성을 보였다. 에탄올 추출물은 $100{\mu}g/ml$의 농도에서 arbutin $10{\mu}g/ml$의 활성보다 높은 저해활성을 보였으나, kojic acid $10{\mu}g/ml$의 활성보다는 낮은 저해활성을 보였다. 또한, 메탄올, 헥산, 부탄올 및 수층 분획물은 $100{\mu}g/ml$의 농도에서 arbutin $10{\mu}g/ml$의 활성과 유사한 저해활성을 보였다. 항산화 활성은 L-ascorbic acid를 양성 대조군으로 하여 수치를 비교하여 나타냈다. 에탄올 추출물 및 수층 분획물의 DPPH 라디칼 소거활성은 다른 시료들보다 비교적 높게 나타났다. 또한, 에탄올 추출물및 수층 분획물은 $500{\mu}g/ml$의 농도에서 LPS에 의해 유도된 iNOS 발현을 각각 82와 80% 감소시켰다. 이러한 결과들로 톳의 에탄올 추출물과 수층 분획물은 미백효과 및 피부 보호효과를 가지는 화장품 소재로 이용 가능할 것으로 기대된다.

Keywords

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