생명과학회지 (Journal of Life Science)

  • 제22권7호
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  • Pages.857-862
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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손바닥 선인장 보충이 고지방식이 흰쥐의 간장, 신장 골격근에 지질괴산화와 SOD단백질 발현에 미치는 영향

Effects of Opuntia humifusa Supplementation on Lipid Peroxidation and SOD Protein Expression in the Liver, Kidney, and Skeletal Muscle of Rats Fed a High-fat Diet

  • 권대근 (선문대학교 스포츠영양학실) ;
  • 강준용 (선문대학교 스포츠영양학실) ;
  • 박진호 (선문대학교 스포츠영양학실) ;
  • 류승필 (경북대학교 레져스포츠학과) ;
  • 송영주 (선문대학교 스포츠영양학실)
  • Kwon, Dae-Keun (Laboratory of Sports Nutrition, Sunmoon University) ;
  • Kang, Jun-Yong (Laboratory of Sports Nutrition, Sunmoon University) ;
  • Park, Jin-Ho (Laboratory of Sports Nutrition, Sunmoon University) ;
  • Ryu, Sung-Pil (Department of Leisure Sports, Kyungpook National University) ;
  • Song, Young-Ju (Laboratory of Sports Nutrition, Sunmoon University)
  • 투고 : 2012.05.08
  • 심사 : 2012.06.13
  • 발행 : 2012.07.30
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초록

본 연구는 안정 시 고지방식이 흰쥐의 신장, 간장 그리고 골격근에서 지질과산화와 SOD 단백질 발현에 손바닥 선인장 보충이 미치는 효과에 대하여 연구하였다. SD계 수컷 흰쥐 16마리를 무작위로 대조군(CG, n=8)과 실험군(EG, n=8)으로 분류하였다. 8주 동안 대조군은 고지방식이를 부하하였으며, 실험군은 5% 손바닥선인장을 보충식 이하였다. 본 실험결과, 간장과 신장의 MDA 농도는 EG군이 CG군에 비해 유의하게 낮게 나타났다(p<0.01). 또한 골격근의 MDA 농도도 EG군이 CG군에 비해 높은 경향이 나타났으나, 통계적으로 유의한 차이는 나타나지 않았다. Cu,Zn-SOD 단백질 발현은 신장에서 EG군이 CG군에 비해 유의하게 증가하였다(p<0.01). Mn-SOD 단백질 발현은 골격근에서 EG군이 CG군에 비해 유의하게 증가하였다(p<0.01). 이상의 결과로부터 손바닥선인장 보충이 고지방식이 흰쥐의 지질과산화 억제작용과 SOD단백질 발현이 기관 특이적인 반응의 항산화 효과가 있는 것으로 나타났다.

This study was conducted to investigate the effects of Opuntia humifusa supplementation on lipid peroxidation and superoxide dismutase (SOD) protein expression at resting state in various organs of rats fed a high-fat diet. Sixteen Sprague-Dawley male rats, 6 weeks of age, were randomly divided into two groups: a control diet group (CG, n=8) and an experimental diet group (EG, n=8). They were given a high-fat diet (CG) or a diet supplemented with 5% of O. humifusa (EG) for 8 weeks. The results showed that the malondialdehyde (MDA) levels of the kidney and the liver were significantly lower in the EG group than in the CG group (p<0.01). In addition, the MDA levels in the skeletal muscle of the EG group tended to be lower than those in the CG group, but this difference was not significant. The Cu, Zn-SOD protein expression in the kidney of the EG group was significantly increased compared with that of the CG group (p<0.01). The Mn-SOD protein expression in the skeletal muscle of the EG group was significantly increased compared with that of the CG group (p<0.01). These results suggest that O. humifusa supplementation has antioxidative properties, which are exerted in a specific organ manner, and that it inhibits the action of lipid peroxidation and the expression of SOD in rats fed a high-fat diet.

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