Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Opuntia humifusa Supplementation on Lipid Peroxidation and SOD Protein Expression in the Liver, Kidney, and Skeletal Muscle of Rats Fed a High-fat Diet

손바닥 선인장 보충이 고지방식이 흰쥐의 간장, 신장 골격근에 지질괴산화와 SOD단백질 발현에 미치는 영향

  • Kwon, Dae-Keun (Laboratory of Sports Nutrition, Sunmoon University) ;
  • Kang, Jun-Yong (Laboratory of Sports Nutrition, Sunmoon University) ;
  • Park, Jin-Ho (Laboratory of Sports Nutrition, Sunmoon University) ;
  • Ryu, Sung-Pil (Department of Leisure Sports, Kyungpook National University) ;
  • Song, Young-Ju (Laboratory of Sports Nutrition, Sunmoon University)
  • 권대근 (선문대학교 스포츠영양학실) ;
  • 강준용 (선문대학교 스포츠영양학실) ;
  • 박진호 (선문대학교 스포츠영양학실) ;
  • 류승필 (경북대학교 레져스포츠학과) ;
  • 송영주 (선문대학교 스포츠영양학실)
  • Received : 2012.05.08
  • Accepted : 2012.06.13
  • Published : 2012.07.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the effects of Opuntia humifusa supplementation on lipid peroxidation and superoxide dismutase (SOD) protein expression at resting state in various organs of rats fed a high-fat diet. Sixteen Sprague-Dawley male rats, 6 weeks of age, were randomly divided into two groups: a control diet group (CG, n=8) and an experimental diet group (EG, n=8). They were given a high-fat diet (CG) or a diet supplemented with 5% of O. humifusa (EG) for 8 weeks. The results showed that the malondialdehyde (MDA) levels of the kidney and the liver were significantly lower in the EG group than in the CG group (p<0.01). In addition, the MDA levels in the skeletal muscle of the EG group tended to be lower than those in the CG group, but this difference was not significant. The Cu, Zn-SOD protein expression in the kidney of the EG group was significantly increased compared with that of the CG group (p<0.01). The Mn-SOD protein expression in the skeletal muscle of the EG group was significantly increased compared with that of the CG group (p<0.01). These results suggest that O. humifusa supplementation has antioxidative properties, which are exerted in a specific organ manner, and that it inhibits the action of lipid peroxidation and the expression of SOD in rats fed a high-fat diet.

본 연구는 안정 시 고지방식이 흰쥐의 신장, 간장 그리고 골격근에서 지질과산화와 SOD 단백질 발현에 손바닥 선인장 보충이 미치는 효과에 대하여 연구하였다. SD계 수컷 흰쥐 16마리를 무작위로 대조군(CG, n=8)과 실험군(EG, n=8)으로 분류하였다. 8주 동안 대조군은 고지방식이를 부하하였으며, 실험군은 5% 손바닥선인장을 보충식 이하였다. 본 실험결과, 간장과 신장의 MDA 농도는 EG군이 CG군에 비해 유의하게 낮게 나타났다(p<0.01). 또한 골격근의 MDA 농도도 EG군이 CG군에 비해 높은 경향이 나타났으나, 통계적으로 유의한 차이는 나타나지 않았다. Cu,Zn-SOD 단백질 발현은 신장에서 EG군이 CG군에 비해 유의하게 증가하였다(p<0.01). Mn-SOD 단백질 발현은 골격근에서 EG군이 CG군에 비해 유의하게 증가하였다(p<0.01). 이상의 결과로부터 손바닥선인장 보충이 고지방식이 흰쥐의 지질과산화 억제작용과 SOD단백질 발현이 기관 특이적인 반응의 항산화 효과가 있는 것으로 나타났다.

Keywords

References

  1. Amin, K. A., Kamel, H. H. and Abd Eltawab, M. A. 2011. Protective effect of Garcinia against renal oxidative stress and biomarkers induced by high fat and sucrose diet. Lipids Health Dis. 14, 10-16.
  2. Amin, K. A. and Nagy, M. A. 2009. Effect of Carnitine and herbal mixture extract on obesity induced by high fat diet in rats1. Diabetol. Metab. Syndr. 6, 1-17.
  3. Bieger, J., Cermak, R., Blank, R., de Boer, V. C., Hollman, P. C., Kamphues, J. and Wolffram, S. 2008. Tissue distribution of quercetin in pigs after long-term dietary supplementation. J. Nutr. 138, 1417-1420.
  4. Buege, J. A. and Aust, S. D. 1978. Microsomal lipid peroxidation. Methods Enzymol. 52, 302-310. https://doi.org/10.1016/S0076-6879(78)52032-6
  5. Cho, J. Y., Park, S. C., Kim, T. W., Kim, K. S., Song, J. C., Kim, S. K., Lee, H. M., Sung, H. J., Park, H. J., Song, Y. B., Yoo, E. S., Lee, C. H. and Rhee, M. H. 2006. Radical scavenging and anti-inflammatory activity of extracts from Opuntia humifusa Raf. J. Pharm. Pharmacol. 58, 113-119. https://doi.org/10.1211/jpp.58.1.0014
  6. Davis, J. M., Murphy, E. A., Carmichael, M. D. and Davis, B. 2009. Quercetin increases brain and muscle mitochondrial biogenesis and exercise tolerance. Am. J. Physiol. Regul. Integr. Comp. Physiol. 296, 1071-1077. https://doi.org/10.1152/ajpregu.90925.2008
  7. de Boer, V. C., Dihal, A. A., van der Woude, H., Arts, I. C., Wolffram, S., Alink, G. M., Rietjens, I. M., Keijer, J. and Hollman, P. C. 2005. Tissue distribution of quercetin in rats and pigs. J. Nutr. 135, 1718-1725.
  8. Diaz, J. P., Navarro, M., Lopez, H. and Lopez, M. C. 1994. Determination of selenium levels in vegetables and fruits by hydride generation atomic absorption spectrometry. J. Agric. Food Chem. 42, 2848-2851. https://doi.org/10.1021/jf00048a036
  9. Galati, E. M., Monforte, M. T., Tripodo, M. M., d'Aquino, A. and Mondello, M. R. 2001. Antiulcer activity of Opuntia ficus-indica (L.) Mill. (Cactaeceae): ultrastructural study. J. Ethnopharmacol. 76, 1-9. https://doi.org/10.1016/S0378-8741(01)00196-9
  10. Goldstein, G. and Nobel, P. S. 1994. Water relations and low-temperature acclimation for cactus species varying in freezing tolerance. Plant Physiol. 104, 675-681.
  11. Gul, M., Kutay, F. Z., Temocin, S. and Hanninen, O. 2000. Cellular and clinical implications of glutathione. Indian J. Exp. Biol. 38, 625-634.
  12. Grundy, S. M. 2004. Obesity, metabolic syndrome, and cardiovascular disease. J. Clin. Endocrinol. Metab. 89, 2595-2600. https://doi.org/10.1210/jc.2004-0372
  13. Gurrieri, S., Miceli, L., Lanza, C. M., Tomaselli, F., Bonomo, R. P. and Rizzarelli, E. 2000. Chemical characteriztion of Sicilian prickly pear (Opuntia ficus indica) and perspectives for the storage of its juice. J. Agric. Food Chem. 48, 5424-5431. https://doi.org/10.1021/jf9907844
  14. Hahm, S. W., Park, J. and Son, Y. S. 2011. Opuntia humifusa stems lower blood glucose and cholesterol levels in streptozotocin-induced diabetic rats. Nutr. Res. 31, 479-487. https://doi.org/10.1016/j.nutres.2011.05.002
  15. Ibanez-Camacho, R. and Roman-Ramos, R. 1979. Hypoglycemic effect of Opuntia catus. Arch. Invest. Med. 30, 433-446.
  16. Kim, Y. J. and Park, T. 2008. Genes are differentially expressed in the epididymal fat of rats rendered obese by a high-fat diet. Nutr. Res. 28, 414-422. https://doi.org/10.1016/j.nutres.2008.03.015
  17. Kojda, G. and Hambrecht, R. 2005. Molecular mechanisms of vascular adaptations to exercise. Physical activity as an effective antioxidant therapy? Cardiovasc. Res. 67, 187-197. https://doi.org/10.1016/j.cardiores.2005.04.032
  18. Kume, S., Uzu, T., Araki, S., Sugimoto, T., Isshiki, K., Chin-Kanasaki, M., Sakaguchi, M., Kubota, N., Terauchi, Y., Kadowaki, T., Haneda, M., Kashiwagi, A. and Koya, D. 2007. Role of altered renal lipid metabolism in the development of renal injury induced by a high-fat diet. J. Am. Soc. Nephrol. 18, 2715-2723 https://doi.org/10.1681/ASN.2007010089
  19. Liu, C. M., Ma, J. Q. and Sun, Y. Z. 2010. Quercetin protects the rat kidney against oxidative stress-mediated DNA damage and apoptosis induced by lead. Environ. Toxicol. Pharmacol. 30, 264-271. https://doi.org/10.1016/j.etap.2010.07.002
  20. Mahboob, M., Rahman, M. F. and Grover, P. 2005. Serum lipid peroxidation and antioxidant enzyme levels in male and female diabetic patients. Singapore Med. J. 46, 322-324.
  21. Martinez-Florez, S., Gonzalez-Gallego, J., Culebras, J. M. and Tunon, M. J. 2002. Flavonoids: properties and anti-oxidizing action. Nutr. Hosp. 17, 271-278.
  22. Michiels, C., Raes, M., Toussaint, O. and Remacle, J. 1994. Importance of Se-glutathione peroxidase, catalase and Cu/Zn-SOD for cell survival against oxidative stress. Free. Radic. Biol. Med. 17, 235-248. https://doi.org/10.1016/0891-5849(94)90079-5
  23. Noeman, S. A., Hamooda, H. E. and Baalash, A. A. 2011. Biochemical study of oxidative stress markers in the liver, kidney and heart of high fat diet induced obesity in rats. Diabetol. Metab. Syndr. 3, 17-24. https://doi.org/10.1186/1758-5996-3-17
  24. Park, E. H., Kahng, J. H. and Paek, E. A. 1998. Studies on the pharmacological actions of cactus: identification of its anti-inflammatory effect. Arch. Pharm. Res. 21, 30-34. https://doi.org/10.1007/BF03216749
  25. Raffaella, C., Francesca, B., Italia, F., Marina, P., Giovanna, L. and Susanna, I. 2008. Alterations in hepatic mitochondrial compartment in a model of obesity and insulin resistance. Obesity 16, 958-964. https://doi.org/10.1038/oby.2008.10
  26. Renugadevi, J. and Prabu, S. M. 2010. Quercetin protects against oxidative stress-related renal dysfunction by cadmium in rats. Exp. Toxicol. Pathol. 62, 471-481. https://doi.org/10.1016/j.etp.2009.06.006
  27. Rice-Evans, C. A., Miller, N. J., Bolwell, P. G., Bramley, P. M. and Pridham, J. B. 1995. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free. Radic. Res. 22, 375-383. https://doi.org/10.3109/10715769509145649
  28. Sen, C. K. 1997. Nutritional biochemistry of cellular glutathione. J. Nutr. Biochem. 8, 660-672. https://doi.org/10.1016/S0955-2863(97)00113-7
  29. Singh, R. J., Goss, S. P., Joseph, J. and Kalyanaraman, B. 1998. Nitration of gamma-tocopherol and oxidation of alpha-tocopherol by copper-zinc superoxide dismutase/H2O2/NO2-: role of nitrogen dioxide free radical. Proc. Natl. Acad. Sci. USA. 95, 12912-12917. https://doi.org/10.1073/pnas.95.22.12912
  30. Sparks, L. M., Xie, H., Koza, R. A., Mynatt, R., Hulver, M. W., Bray, G. A. and Smith, S. R. 2005. A high-fat diet coordinately downregulates genes required for mitochondrial oxidative phosphorylation in skeletal muscle. Diabetes 54, 1926-1933. https://doi.org/10.2337/diabetes.54.7.1926
  31. Sreekumar, R., Unnikrishnan, J., Fu, A., Nygren, J., Short, K. R., Schimke, J., Barazzoni, R. and Nair, K. S. 2002. Impact of high-fat diet and antioxidant supplement on mitochondrial functions and gene transcripts in rat muscle. Am. J. Physiol. Endocrinol. Metab. 282, 1055-1061.
  32. Tesoriere, L., Butera, D., Pintaudi, A. M., Allegra, M. and Livrea, M. A. 2004. Supplementation with cactus pear (Opuntia ficus-indica) fruit decreases oxidative stress in healthy humans: a comparative study with vitamin C1-3. Am. J. Clin. Nutr. 80, 391-395.
  33. Tokyol, C., Yilmaz, S., Kahraman, A., Cakar, H. and Polat, C. 2006. The effects of desferrioxamine and quercetin on liver injury induced by hepatic ischaemia-reperfusion in rats. Acta. Chir. Belg. 106, 68-72.
  34. Trejo-Gonzalez, A., Gabriel-Ortiz, G., Puebla-Perez, A. M., Huizar-Contreras, M. D., Munguia-Mazarigos, M. R. and Mejia-Arreguins, C. E. 1996. A purified extract from prickly pear cactus (Opuntia fulginosa) controls experimentally induced diabetes in rats. J. Ethnopharmacol. 55, 27-33. https://doi.org/10.1016/S0378-8741(96)01467-5
  35. Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T., Mazur, M. and Telser, J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 39, 44-84. https://doi.org/10.1016/j.biocel.2006.07.001
  36. Valko, M., Rhodes, C. J., Moncol, J., Izakovic, M. and Mazur, M. 2006. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem. Biol. Interact. 160, 1-40. https://doi.org/10.1016/j.cbi.2005.12.009