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The Effects of Genetically Modified Crops on Soil Microbial Community

유전자변형 작물이 토양 미생물상에 미치는 영향

  • 이기종 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 오성덕 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 손수인 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 류태훈 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 박종석 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 이장용 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 조현석 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 안병옥 (농촌진흥청 국립농업과학원 농업생명자원부)
  • Received : 2012.05.18
  • Accepted : 2012.06.25
  • Published : 2012.06.30

Abstract

BACKGROUND: Genetically modified (GM) crops must receive relevant regulator's authorization before they can be sold as seed or used food, feed and processing. Before approving any GM crop, the relevant government ministries are required to examine environmental risk assessment to make scientifically sound and socially acceptable decisions. But one of the least studied and understood areas in the environmental risk assessment of GM crops are their impact on soil microbial community. METHODS AND RESULTS: Recently, advanced methods have been developed to characterize the soil microbial community in various environments. In this study, the culture-dependent and culture-independent technical approaches for profiling soil microbial communities are summarized and their applicability to assess GM crops are discussed. CONCLUSION(S): We concluded that the effect of GM crops on soil microbial community need to be assessed on a case by case basis. The combination of culture-dependent and culture-independent method was necessary for reliable and detailed assessment of effect of GM crops on soil microbial community.

유전자변형 작물을 종자로 판매하거나 식품, 사료 혹은 가공용으로 이용하기 위해서는 반드시 관련 기관의 승인을 받아야 한다. 관련부처에서는 유전자변형 작물의 승인에 앞서 환경위해성 평가 자료가 과학적으로 타당한지 검토한다. 환경위해성 평가 중 유전자변형 작물이 토양 미생물 군집에 미치는 영향은 충분히 연구되지 못한 분야이다. 최근 토양 환경내 미생물 군집의 특성을 연구하기 위한 발전된 방법들이 개발되고 있다. 배양에 의존적인 또는 비의존적인 기술에 의한 토양 미생물의 군집 특성을 조사한 연구와 유전자변형 작물의 환경위해성 평가 적용 가능성을 고찰하였다. 유전자변형 작물의 토양미생물 영향 평가는 사안별 평가 원칙에 의해 이루어져야 한다. 신뢰할 수 있고 상세한 토양 미생물 평가가 이루어지기 위해서는 다양한 분석 방법의 조합이 필요하다.

Keywords

References

  1. Ahrenholtz, I., Harms, K., de Vries, J., Wackernagel, W., 2000. Increased killing of Bacillus subtilis on the hair roots of transgenic T4 lysozyme-producing potatoes, Appl. Environ. Microb. 66, 1862-1865. https://doi.org/10.1128/AEM.66.5.1862-1865.2000
  2. Angle, J. S., 1994. Release of Transgenic Plants-Biodiversity and Population-Level Considerations, Mol. Ecol. 3, 45-50.
  3. Barton, J. E., Dracup, M., 2000. Genetically modified crops and the environment, Agron. J. 92, 797-803. https://doi.org/10.2134/agronj2000.924797x
  4. Baumgarte, S., Tebbe, C. C., 2005. Field studies on the environmental fate of the Cry1Ab Bt-toxin produced by transgenic maize (MON810) and its effect on bacterial communities in the maize rhizosphere, Mol. Ecol. 14, 2539-2551. https://doi.org/10.1111/j.1365-294X.2005.02592.x
  5. Bruinsma, M., Kowalchuk, G. A., van Veen, J. A., 2003. Effects of genetically modified plants on microbial communities and processes in soil, Biol. Fert. Soils. 37, 329-337.
  6. Clausen, M., Krauter, R., Schachermayr, G., Potrykus, I., Sautter, C., 2000. Antifungal activity of a virally encoded gene in transgenic wheat, Nat. Biotech. 18, 446-449. https://doi.org/10.1038/74521
  7. Daniell, H., Muthukumar, B., Lee, S. B., 2001. Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection, Curr. Genet. 39, 109-116. https://doi.org/10.1007/s002940100185
  8. De Vries, J., Harms, K., Broer, I., Kriete, G., Mahn, A., Düring, K., Wackernagel, W., 1999. The bacteriolytic activity in transgenic potatoes expressing a chimeric T4 lysozyme gene and the effect of T4 lysozyme on soil-and phytopathogenic bacteria, Syst. Appl. Microbiol. 22, 280-286. https://doi.org/10.1016/S0723-2020(99)80075-7
  9. Di Giovanni, G. D., Watrud, L. S., Seidler, R. J., Widmer, F., 1999. Comparison of parental and pransgenic alfalfa rhizosphere bacterial communities using biolog GN metabolic fingerprinting and enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR), Microb. Ecol. 37, 129-139. https://doi.org/10.1007/s002489900137
  10. Donegan, K. K., Palm, C. J., Fieland, V. J., Porteous, L. A., Ganio, L. M., Schaller, D. L., Bucao, L. Q., Seidler, R. J., 1995. Changes in levels, species and DNA fingerprints of soil-microorganisms associated with cotton expressing the Bacillus thuringiensis Var. Kurstaki endotoxin, Appl. Soil. Ecol. 2, 111-124. https://doi.org/10.1016/0929-1393(94)00043-7
  11. Donegan, K. K., Seidler, R. J., Fieland, V. J., Schaller, D. L., Palm, C. J., Ganio, L. M., Cardwell, D. M., Steinberger, Y., 1997. Decomposition of genetically engineered tobacco under field conditions: Persistence of the proteinase inhibitor I product and effects on soil microbial respiration and protozoa, nematode and microarthropod populations, J. Appl. Ecol. 34, 767-777. https://doi.org/10.2307/2404921
  12. Dunfield, K. E., Germida, J. J., 2001. Diversity of bacterial communities in the rhizosphere and root interior of field-grown genetically modified Brassica napus, Fems Microbiol. Ecol. 38, 1-9. https://doi.org/10.1111/j.1574-6941.2001.tb00876.x
  13. Filion, M., 2008. Do transgenic plants affect rhizobacteria populations?, Microb. Biotechnol. 1, 463-475. https://doi.org/10.1111/j.1751-7915.2008.00047.x
  14. Germida, J. J., Dunfield, K. E., 2004. Impact of genetically modified crops on soil-and plantassociated microbial communities, J. Environ. Qual. 33, 806-815. https://doi.org/10.2134/jeq2004.0806
  15. Griffiths, B. S., Geoghegan, I. E., Robertson, W. M., 2000. Testing genetically engineered potato, producing the lectins GNA and Con A, on non-target soil organisms and processes, J. Appl. Ecol. 37, 159-170.
  16. Heuer, H., Kroppenstedt, R. M., Lottmann, J., Berg, G., Smalla, K., 2002. Effects of T4 lysozyme release from transgenic potato roots on bacterial rhizosphere relative to communities are negligible natural factors, Appl. Environ. Microb. 68, 1325-1335. https://doi.org/10.1128/AEM.68.3.1325-1335.2002
  17. Heuer, H., Smalla, K., 1999. Bacterial phyllosphere communities of Solanum tuberosum L. and T4-lysozyme-producing transgenic variants, Fems Microbiol Ecol 28, 357-371. https://doi.org/10.1111/j.1574-6941.1999.tb00590.x
  18. James, C., 2011. Global Status of Commercialized Biotech/GM Crops: 2011 , No. 43., ISAAA, Ithaca, NY.
  19. Kirk, J. L., Beaudette, L. A., Hart, M., Moutoglis, P., Khironomos, J. N., Lee, H., Trevors, J. T., 2004. Methods of studying soil microbial diversity, J Microbiol Meth 58, 169-188. https://doi.org/10.1016/j.mimet.2004.04.006
  20. Kremer, R. J., Means, N. E., Kim, S. J., 2005. Glyphosate affects soybean root exudation and rhizosphere micro-organisms, Int J Environ an Ch 85, 1165-1174. https://doi.org/10.1080/03067310500273146
  21. Lee EH, Park HJ, Jo YS, Ryu HW, KS, C., 2010. Application of methodology for microbial community analysis to gas-phase biofilters, Korean J. Chem. Eng. 48, 147-156.
  22. Libiakova, G., Jorgensen, B., Palmgren, G., Ulvskov, P., Johansen, E., 2001. Efficacy of an introncontaining kanamycin resistance gene as a selectable marker in plant transformation, Plant Cell Rep 20, 610-615. https://doi.org/10.1007/s002990100375
  23. Liu, B., Zeng, Q., Yan, F., Xu, H., Xu, C., 2005. Effects of transgenic plants on soil microorganisms, Plant Soil 271, 1-13. https://doi.org/10.1007/s11104-004-1610-8
  24. Lorenz, M. G., Blum, S. A. E., Wackernagel, W., 1997. Mechanism of retarded DNA degradation and prokaryotic origin of DNases in nonsterile soils, Syst Appl Microbiol 20, 513-521. https://doi.org/10.1016/S0723-2020(97)80021-5
  25. Losey, J. E., Rayor, L. S., Carter, M. E., 1999. Transgenic pollen harms monarch larvae, Nature 399, 214-214.
  26. Lottmann, J., Berg, G., 2001. Phenotypic and genotypic characterization of antagonistic bacteria associated with roots of transgenic and non-transgenic potato plants, Microbiol Res 156, 75-82. https://doi.org/10.1078/0944-5013-00086
  27. Lukow, T., Dunfield, P. F., Liesack, W., 2000. Use of the T-RFLP technique to assess spatial and temporal changes in the bacterial community structure within an agricultural soil planted with transgenic and non-transgenic potato plants, Fems Microbiol Ecol 32, 241-247. https://doi.org/10.1111/j.1574-6941.2000.tb00717.x
  28. Lynch, J. M., Benedetti, A., Insam, H., Nuti, M. P., Smalla, K., Torsvik, V., Nannipieri, P., 2004. Microbial diversity in soil: ecological theories, the contribution of molecular techniques and the impact of transgenic plants and transgenic microorganisms, Biol Fert Soils 40, 363-385. https://doi.org/10.1007/s00374-004-0784-9
  29. Maizel, A., Weigel, D., 2004. Temporally and spatially controlled induction of gene expression in Arabidopsis thaliana , Plant J 38, 164-171. https://doi.org/10.1111/j.1365-313X.2004.02027.x
  30. Marschner, P., Crowley, D., Yang, C. H., 2004. Development of specific rhizosphere bacterial communities in relation to plant species, nutrition and soil type, Plant Soil 261, 199-208. https://doi.org/10.1023/B:PLSO.0000035569.80747.c5
  31. Neal, J. L., Atkinson, T. G., Larson, R. I., 1970. Changes in the rhizosphere microflora of spring wheat induced by disomic substitution of a chromosome, Can J Microbiol 16, 153-158. https://doi.org/10.1139/m70-027
  32. Nielsen, K. M., van Elsas, J. D., Smalla, K., 2000. Transformation of Acinetobacter sp strain BD413 (pFG4 Delta nptII) with transgenic plant DNA in soil microcosms and effects of kanamycin on selection of transformants, Appl Environ Microb 66, 1237-1242. https://doi.org/10.1128/AEM.66.3.1237-1242.2000
  33. Nocker, A., Burr, M., Camper, A. K., 2007. Genotypic microbial community profiling: A critical technical review, Microbial Ecology 54, 276-289. https://doi.org/10.1007/s00248-006-9199-5
  34. Oger, P., Petit, A., Dessaux, Y., 1997. Genetically engineered plants producing opines alter their biological environment, Nat Biotechnol 15, 369-372. https://doi.org/10.1038/nbt0497-369
  35. Paget, E., Lebrun, M., Freyssinet, G., Simonet, P., 1998. The fate of recombinant plant DNA in soil, Eur J Soil Biol 34, 81-88. https://doi.org/10.1016/S1164-5563(99)90005-5
  36. Rodenburg, K. W., Degroot, M. J. A., Schilperoort, R. A., Hooykaas, P. J. J., 1989. Single-Stranded-DNA Used as an Efficient New Vehicle for Transformation of Plant-Protoplasts, Plant Mol Biol 13, 711-719. https://doi.org/10.1007/BF00016026
  37. Saxena, D., Flores, S., Stotzky, G., 1999. Transgenic plants-Insecticidal toxin in root exudates from Bt corn, Nature 402, 480-480.
  38. Saxena, D., Stotzky, G., 2000. Insecticidal toxin from Bacillus thuringiensis is released from roots of transgenic Bt corn in vitro and in situ, Fems Microbiol Ecol 33, 35-39. https://doi.org/10.1111/j.1574-6941.2000.tb00724.x
  39. Schmalenberger, A., Tebbe, C. C., 2002. Bacterial community composition in the rhizosphere of a transgenic, herbicide-resistant maize (Zea mays ) and comparison to its non-transgenic cultivar Bosphore, Fems Microbiol Ecol 40, 29-37. https://doi.org/10.1111/j.1574-6941.2002.tb00933.x
  40. Siciliano, S. D., Germida, J. J., 1999. Taxonomic diversity of bacteria associated with the roots of field-grown transgenic Brassica napus cv. Quest, compared to the non-transgenic B. napus cv. Excel and B. rapa cv. Parkland, Fems Microbiol Ecol 29, 263-272. https://doi.org/10.1111/j.1574-6941.1999.tb00617.x
  41. Siciliano, S. D., Theoret, C. M., de Freitas, J. R., Hucl, P. J., Germida, J. J., 1998. Differences in the microbial communities associated with the roots of different cultivars of canola and wheat, Can J Microbiol 44, 844-851. https://doi.org/10.1139/w98-075
  42. Sims, S. R., Ream, J. E., 1997. Soil inactivation of the Bacillus thuringiensis subsp kurstaki CryIIA insecticidal protein within transgenic cotton tissue: Laboratory microcosm and field studies, J Agr Food Chem 45, 1502-1505. https://doi.org/10.1021/jf960647w
  43. Smalla, K., Gebhard, F., 1999. Monitoring field releases of genetically modified sugar beets for persistence of transgenic plant DNA and horizontal gene transfer, Fems Microbiol Ecol 28, 261-272. https://doi.org/10.1111/j.1574-6941.1999.tb00581.x
  44. Tilman, D., Downing, J. A., 1994. Biodiversity and stability in grasslands, Nature 367, 363-365. https://doi.org/10.1038/367363a0
  45. Widmer, F., Seidler, R. J., Donegan, K. K., Reed, G. L., 1997. Quantification of transgenic plant marker gene persistence in the field, Mol Ecol 6, 1-7. https://doi.org/10.1046/j.1365-294X.1997.00145.x

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