Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지
DOI QR코드

DOI QR Code

Acute Oral Toxicity and Skin Irritation Studies on Natural Dyes Extracted from Chrysanthemum

국화로부터 추출한 천연염료에 대한 급성경구독성 및 피부자극성 시험에 대한 연구

  • Kwon, Jung-Ki (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • An, In-Jung (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Lee, Jin-Seok (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Hae-Ri (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Park, Ha-Seung (Yesan Chrysanthemum Experiment Station) ;
  • Kim, Dong-Chan (Yesan Chrysanthemum Experiment Station) ;
  • Choi, Byung-Jun (Yesan Chrysanthemum Experiment Station) ;
  • Lee, Kyu-Min (Department of Plant Science and Technology, Sangmyung University) ;
  • Park, Yong-Jin (Department of Plant Resources, Kongju National University) ;
  • Jung, Ji-Youn (Department of Companion and Laboratory Animal Science, Kongju National University)
  • Received : 2012.05.31
  • Accepted : 2012.06.18
  • Published : 2012.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was designed to evaluate to acute oral toxicity and skin irritation of Chrysanthemum dye in Sprague-Dawley (SD) rats. SD rats were orally treated with Chrysanthemum dye at a dose of 0, 1 and 2 ml/kg body weight. After oral administration, the rats were observed for 14days. In primary skin irritation test, SD rats were dermally treated with Chrysanthemum dye and observed for 3 days. To ensure the safety of Chrysanthemum dye such as the following were observed and tested. We examined the body weight, the feed intake, the clinical signs, the ophthalmological test, the histopathological test, the mortality and skin irritation. As a result, no significant differences were found in body weight, feed intake and histopathological test between control and Chrysanthemum dye treated group. In the result of skin irritation test, Chrysanthemum dye did not induce erythema and edema after topical application. Primary irritation index was "0" in the test. Therefore, it is suggested that Chrysanthemum dye has no effect on acute toxicity and side effect in SD rats and is non-irritant material based on the score "0" of primary irritation index.

본 연구는 SD 계통의 랫드에서 국화 염료인 백색국화, 백색국화 잎과 줄기, 황색국화의 급성경구독성 및 피부자극성을 평가하기 위해서 시행하였다. 수컷 랫드에 처치한 물질은 백색국화, 백색국화 잎과 줄기, 황색국화를 각각 1 ml/kg, 2 ml/kg를 D.W에 용해시켜 단회 경구 투여하였으며, 수컷 SD rats에 투여 후 14일 동안 관찰하였으며, 피부자극성 시험은 피부의 찰과 및 비찰과부의 대조부, 처치부에 국화 염료를 처치하여 3일 동안 관찰하였다. 단회 경구 투여 및 피부 자극성 시험 후 국화 염료의 안전성을 확인하기 위해 체중과 사료 섭취량, 임상증상, 안과학적 검사, 사망률, 피부자극성을 관찰하고, 랫드 피부에서의 병리조직학적 변화를 관찰하였다. 체중, 사료섭취량, 병리조직학적 변화가 대조군과 비교시 유의적인 변화가 나타나지 않았다. 또한 피부자극성을 확인 한 결과 1차 자극 지수가 0으로 비자극성 물질로 분류되었다. 따라서 본 연구를 통하여 국화에서 추출한 염료가 급성 경구독성 시 안전한 물질이며, 피부 자극성을 야기 하지않는 물질로 평가된다.

Keywords

References

  1. Bae, S.E., Oh, T.K. and Park, S.C.: Skin Irritation of natural dyes extracted from onion(Allium cepa). Korean. J. Toxicol, 13, 161-165 (1997).
  2. 이종남: 우리가 정말 알아야 할 천연염색. 현암사, 서울, pp. 16 (2004).
  3. Shin, H.J.: A trend in research and development of natural gardenia pigments. Korean J. Biotechnol. Bioeng. 22, 271-277 (2007)
  4. Jeong, Y.O.: The dyeability of natural dye extracted from chesnut shell. The Korean Journal Of Community Living Science, 8, 83-91 (1997).
  5. Woo, J.H., Shin, S.L., Jeong, H.S. and Lee, C.H.: Antioxidant effect of extracts obtained from three chrysanthemum species. J. Korean. Soc. Food. Sci. Nutr, 39, 631-636 (2010). https://doi.org/10.3746/jkfn.2010.39.4.631
  6. Lee, H.J., Hwang, Y.I., Park, E.J. and Choi, S.U.: Antihepatotoxic and antigenotoxic effects of herb tea composed of chrysanthemum morifolium ramat. J Korean Soc Food. Sci. Nutr, 40, 78-83 (2011). https://doi.org/10.3746/jkfn.2011.40.1.078
  7. Collins, R.A., Ng, T.B., Fong, W.P., Wan, C.C. and Yeung, H.W.: A comparison of human immunodeficiency virus type 1 inhibition by partially purified aqueous extracts of Chinese medicinal herbs. Life. Sci, 60, 345-351 (1997).
  8. Hu, C.Q., Chen, K., Shi, Q., Kilkuskie, R.E., Cheng, Y.C. and Lee, K.H.: Anti-AIDS agents, Acacetin-7-O-beta-Dgalactopyranoside, an anti-HIV principle from Chrysanthemum morifolium and a structure-activity correlation with some related flavonoids. J. Nat. Prod, 57, 42-51 (1994). https://doi.org/10.1021/np50103a006
  9. Sassi, A.B., Harzallah-Skhiri, F., Bourgougnon, N. and Aouni, M.: Antimicrobial activities of four tunisian chrysanthemum species. Ind. J. Med. Res, 127, 183-192 (2008).
  10. Marongiu, B., Piras, A., Porcedda, S., Tuveri, E., Laconi, S., Deidda, D. and Maxia, A.: Chemical and biological comparisons on supercritical extracts of tanacetum cinerariifolium (Trevir) Sch. Bip. with three related species of chrysanthemums of sardinia (Italy). Nat. Prod. Res, 23, 190-199 (2009). https://doi.org/10.1080/14786410801946221
  11. 국립수의과학검역원: 동물용의약품 등 독성시험지침, 고시 제2009-18호, 국립수의과학검역원, 안양 (2006).
  12. Rush, R.E., Bonnette, K.L., Douds, D.A. and Merriman, T.N.: Dermal irritation and sensitization. In CRC Handbook of toxicology, 2nd Ed. (Derelanko, M.J. and Hollinger, M.A.eds.) CRC Press, Boca Raton, pp. 105-162 (1995).
  13. Ma, J.Y., Park, H.Y., Choi, H., Zee, O.P. and Lee, J.H.: Acute toxicity study on Mud Chrysanthemum indicum in mice. Kor. J. Herbology, 23, 81-85 (2008).
  14. Ma, H.J., Shin, H.K., Yu, Y.B., Park, H.Y. and Ma, J.Y.: Dermal irritation study of Mud Chrysanthemum extract in rabbits. Mol. Cell Toxicol, 3, 125-129 (2007).

Cited by

  1. Antioxidant and Antiobesity Activity of Natural Color Resources vol.24, pp.6, 2014, https://doi.org/10.5352/JLS.2014.24.6.633
  2. Chromaticity Analysis of Natural Dyes Extracted from Sappan Wood, Gardenia, and Mugwort vol.27, pp.3, 2016, https://doi.org/10.14478/ace.2016.1044