Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Evaluation of Selective Media for Isolation of Staphylococcus aureus from Agricultural Products

농산물 중 Staphylococcus aureus의 분리를 위한 선택배지 평가

  • Kim, Se-Ri (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Lee, Seo-Hyun (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Seo, Min-Kyoung (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Kim, Won-Il (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Park, Kyeong-Hun (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Yun, Hye-Jeong (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Yoon, Yo-Han (Department of Food &Nutrition, Sookmyung Women's University) ;
  • Yoo, Soon-Young (Seoul Regional Korea Food & Drug Administration) ;
  • Ryu, Kyoung-Yul (R&D Evaluation Division, Research Policy Bureau, RDA) ;
  • Yun, Jong-Chul (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Kim, Byung-Seok (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA)
  • 김세리 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 이서현 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 서민경 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 김원일 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 박경훈 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 윤혜정 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 윤요한 (숙명여자대학교 식품영양학과) ;
  • 유순영 (서울지방식약청) ;
  • 류경열 (농촌진흥청 연구정책국 평가관리과) ;
  • 윤종철 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 김병석 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과)
  • Received : 2012.03.26
  • Accepted : 2012.06.13
  • Published : 2012.06.30
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Abstract

Five kinds of selective media, such as mannitol salt agar (MSA), Baird-Parker agar (BPA), Baird-Parker supplemented with rabbit plasma fibrinogen (BPA+RPF), CHROMagar Staphylococcus aureus (CSA), and Petrifilm Staph Express count system (Petrifilm), were compared to recommend the optimum selective media for isolation of Staphylococcus aureus from agricultural products. Seventy four target and non target bacteria were inoculated on five selective media to analyze sensitivity and specificity. In the recovery test of injured S. aureus cells, S. aureus was exposed to acid (1% lactic acid for 10 min), heat ($60^{\circ}C$ for 90s), and cold ($-20^{\circ}C$ for 1h) conditions. And artificially contaminated agricultural products (iceberg lettuce, green pepper, and cherry tomato) was enumerated on five selective media. The sensitivity of BPA+RPF, CSA, Petrifilm, MSA, and BPA were 100%, 100%, 100%, 90.5%, 90.5%, respectively. In addition, the specificity of BPA+RPF, CSA, MSA, BPA and Petrifilm were 100%, 100%, 84.6%, 75.0%, 67.3%, respectively. However, no difference among five selective media was observed in recovery on injured S. aureus cell and enumeration from agricultural products. This results suggest that BPA+RPF and CSA are the optimum media for detection of S. aureus from agricultural products.

본 연구는 농산물로부터 S. aureus분리에 적합한 배지를 선발하고자 난황첨가 mannitol salt agar (MSA), 난황과 tellurite과 첨가된 Barid-Parker agar (BPA), 토끼혈장이 첨가된 Barid-Parker agar (BPA+RPF), 3M petrifilm Staph Express count plates (Petriflm) 및 CHROMagar Staphylococcus aureus (CSA)의 민감도, 특이도, 손상된 세포에 대한 회복능, 농산물로부터 회수율에 대하여 비교 실험을 하였다. 민감도와 특이도를 조사하기 위하여 S. aureus 21주, Stapylococcus spp. 20주, 그 외 식중독균 33주를 각 선택배지에 접종하였으며 손상된 세포에 대한 회복능은 조사를 위하여 열($60^{\circ}C$, 90초), 산(1% lactic acid, 10분), 저온($-20^{\circ}C$, 1시간) 처리하였다. 또한 양상추, 토마토, 고추에 S. aureus를 4.0 log CFU/g농도로 접종하고 각 선택배지에 도말하였다. 민감도는 BPA+RPF(100%) = CSA(100%)=petrifilm(100%) > MSA(90.5%) > BPA(90.5%), 특이도는 BPA+RPF(100%) = CSA(100%) > MSA(84.6%) > BPA(75.0%) > petrifilm(67.3%) 순으로 나타났다. 한편, 손상된 세포의 회복능과 농산물에서 회수율은 5종의 배지에서 차이가 없었다. 따라서 농산물 중 S. aureus를 분리할 시 BPA+RPF와 CSA를 사용하는 것이 가장 효과적일 것으로 판단된다.

Keywords

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