한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제27권2호
  • /
  • Pages.141-145
  • /
  • 2012
  • /
  • 1229-1153(pISSN)
  • /
  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
권호 표지
DOI QR코드

DOI QR Code

Genotoxicity of Zizyphi Spinosi Semen in Bacterial Reverse Mutation (Ames) Test, Chromosomal Aberration and Micronucleus Test in Mice

  • 투고 : 2012.01.16
  • 심사 : 2012.05.04
  • 발행 : 2012.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Zizyphi spinosi semen (Z. spinosi) has been used in traditional Chinese medicine for the treatment of rheumatoid arthritis and wounds. However, toxicity in high doses was often observed due to the presence of alkaloids. This study was conducted to investigate the potential genotoxicity of Z. spinosi in vitro and in vivo. This was examined by the Bacterial reverse mutation (Ames) test using Salmonella typhimurium TA98, TA100, TA1535, TA1537 and Escherichia coli WP2uvrA, Chromosomal aberration was investigated using Chinese hamster lung cells and the micronucleus test using mice. Z. Spinosi did not induce mutagenicity in the Ames test, and it did not produce chromosomal aberration in Chinese hamster lung cells with and without metabolic activation, nor in the micronucleated polychromatic erythrocytes in the bone marrow cells in mice. Based on these results, it is concluded that Z. spinosi does not have mutagenic potential under the conditions examined in each study.

키워드

참고문헌

  1. Rates, S.M.K.: Plants as source of drugs. Toxicon, 39, 603-613 (2001). https://doi.org/10.1016/S0041-0101(00)00154-9
  2. Zhou, S., Koh, H., Gao, Y., Gong, Z. and Lee, E.J.D.: Herbal bioactivation: The good, bad and the egly. Life Sci.,; 74, 935-968 (2004). https://doi.org/10.1016/j.lfs.2003.09.035
  3. Park, J.H., Lee, H.J., Koh, S.B., Ban, J.Y. and Seong, Y.H.:Protection of NMDA-induced neuronal cell damage by methanol extract of zizyphi spinosi semen in cultured rat cerebellar granule cells. J Ethnopharmacol, 95(1), 39-45 (2004). https://doi.org/10.1016/j.jep.2004.06.011
  4. Huang, X., Kojima-Yuasa, A., Norikura, T., Kennedy, D.O., Hasuma, T. and Matsui-Yuasa, I.: Mechanism of the anticancer activity of Zizyphus jujube in HepG2 cells. Am. J. Clin. Med., 35(3), 517-532 (2007). https://doi.org/10.1142/S0192415X0700503X
  5. Huang, X., Kojima-Yuasa, A., Xu, S., Norikura, T., Kennedy, D.O., Hasuma, T. and Matsui-Yuasa, I.: Green tea extract enhances the selective cytotoxic activity of Zizyphus jujube extracts in HepG2 cells. Am. J. Clin. Med., 36(4), 729-744 (2008). https://doi.org/10.1142/S0192415X08006193
  6. Tetali, P., Waghchaure, C., Daswani, P.G., Antia, N.H. and Birdi, T.J. Ethnobotanical survey of antidiarrhoeal plants of Parinche valley, pune district Maharashtra, India. J. Ethnopharmacol., 123(2), 229-236 (2009). https://doi.org/10.1016/j.jep.2009.03.013
  7. Zhang, H., Jiang, L., Ye, S., Ye, Y. and Ren, F. Systematic evaluation of antioxidant capacities of the ethanolic extract of different tissues of jujube (Ziziphus jujube Mill.) from China. Food Chem. Toxicol., 48(6), 1461-1465 (2010). https://doi.org/10.1016/j.fct.2010.03.011
  8. Yu, L., Jiang, B.P., Luo, D., Shen, X.C., Guo, S., Duan, J.A. and Tang, Y.P. Bioactive components in the fruits of Ziziphus jujube Mill. Against the inflammatory irritant action of Euphorbia plants. Phytomedicine. (2011). [Epub ahead of print]
  9. Wang, F., Liu, X., Chen, Y. and Wang. L. Characterization of Fatty oil of zizyphi spinosi semen obtained by supercritical fluid extraction. J. American oil chemist's society, 88(4), 467-472 (2011). https://doi.org/10.1007/s11746-010-1700-0
  10. Matsuda, H., Murakami, T., Ikebata, A., Yamahara, J. and Yoshikawa, M. Bioactive saponins and glycosides. XIV. Structure elucidation and immunological adjuvant activity of novel protojujubogenin type triterpene bisdesmosides, protojujubosides A, B, and B1, from the seeds of Zizyphus jujuba var. spinosa (Zizyphi Spinosi Semen). Chem. Pharm. Bull., 47(12), 1744-1748 (1999). https://doi.org/10.1248/cpb.47.1744
  11. Yoshikawa, M., Murakami, T., Ikebata, A., Wakao, S., Murakami, N., Matsuda, H. and Yamahara, J. Bioactive saponins and glycosides. X. On the constituents of zizyphi spinosi semen, the seeds of Zizyphus jujuba Mill. var. spinosa Hu (1): structures and histamine release-inhibitory effect of jujubosides A1 and C and acetyljujuboside B. Chem. Pharm. Bull., 45(7), 1186-1192 (1997). https://doi.org/10.1248/cpb.45.1186
  12. Heo, H.J., Park, Y.J., Suh, Y. M., Choi, S.J., Kim, M.J., Cho, H.Y., Chang, Y.J., Hong, B., Kim, H.K., Kim, E., Kim, C.J., Kim, B.G. and Shin, D.H. Effects of Oleamide on Choline Acetyltransferase and Cognitive Activities. Biosci. Biotechnol, Biochem., 67, 1284-1291 (2003) https://doi.org/10.1271/bbb.67.1284
  13. Tripathi, M., Pandey, M.B., Jha, R.N., Pandey, V.B., Tripathi, P.N. and Singh, J.P. Cyclopeptide alkaloids from Zizyphus jujuba. Fitoterapia, 72(5), 507-510 (2001). https://doi.org/10.1016/S0367-326X(01)00278-7
  14. Guo, S., Duan, J.A., Tang, Y., Qian, Y., Zhao, J., Qian, D., Su, S. and Shang, E. Simultaneous qualitative and quantitative analysis of triterpenic acids, saponins and flavonoids in the leaves of two Ziziphus species by HPLC-PDA-MS/ELSD. J. Pharm. Biomed. Anal., 56(2), 264-270 (2011). https://doi.org/10.1016/j.jpba.2011.05.025
  15. Fu, J.Y., Qian, L.B., Zhu, L.G., Liang, H.T., Tan, Y.N., Lu, H.T., Lu, J.F., Wang, H.P. and Xia, Q. Betulinic acid ameliorates endothelium-dependent relaxation in L-NAME-induced hypertensive rats by reducing oxidative stress. Eur. J. Pharm. Sci., 44(3), 385-391 (2011). https://doi.org/10.1016/j.ejps.2011.08.025
  16. Yoon, S.R., Jo, Y.J., Yang, S., Kim, Y.B., Nam, S.Y., Kim, H.C., Hong, J.T. and Oh, K.W. Sanjoinine A isolated from Semen Zizyphi Spinosi protects against kainic acid-induced convulsions. Arch. Pharm. Res., 32(11), 1515-1523 (2009). https://doi.org/10.1007/s12272-009-2103-3
  17. Maron, D.M. and Ames, B.N. Revised methods for the salmonella mutagenicity test. Mutation Res., 113, 173-215 (1983). https://doi.org/10.1016/0165-1161(83)90010-9
  18. Yahagi, T., Nagao, M., Seino, Y., Matsushima, T., Sugimura, T. and Okada, M. Mutagenicies of N-nitrosoamines of Salmonella. Mutation Res., 48, 121-130 (1977). https://doi.org/10.1016/0027-5107(77)90151-8
  19. Ishidate, M.J. and Odshima, S. Chromosome tests with 134 compounds on Chinese hamster cells in vitro a screening for chemical carcinogens. Mutation Res., 48, 337-354 (1977). https://doi.org/10.1016/0027-5107(77)90177-4
  20. Heddle, J.A. A rapid in vivo test for chromosomal damage. Mutation Res., 18, 187-190 (1973). https://doi.org/10.1016/0027-5107(73)90035-3
  21. MacGregor, J.T., Heddle, J.A., Hite, M., Margolin, G.H., Ramel, C., Salamone, M.F., Tice, R.R. and Wild, D. Guidelines for the conduct of micronucleus assays in mammalian bone marrow erythrocytes. Mutation Res., 189, 103-112 (1987). https://doi.org/10.1016/0165-1218(87)90016-4
  22. Phillipson, J.D. Phytochemistry and pharmacognosy. Phytochemistry, 68, 2960-2972 (2007). https://doi.org/10.1016/j.phytochem.2007.06.028
  23. Bast, A., Chandler, R.F., Choy, P.C., Delmulle, L.M., Gruenwald, J., Halkes, S.B.A., Keller, K., Keoman, J.H., Peters, P. and Przyrembel, H. Botanical health products, positioning and requirements for effective and safe use. Environ. Toxicol. Pharmacol., 12, 195-211 (2002). https://doi.org/10.1016/S1382-6689(02)00035-2
  24. Mortelmans, K. and Zeiger, E. The Ames Salmonella/microsome mutagenicity assay. Mutation Res., 455, 29-60 (2000). https://doi.org/10.1016/S0027-5107(00)00064-6
  25. Ahmad, B., Khan, I., Bashir, S., Azam, S. and Ali, N. The antifungal, cytotoxic, antitermite and insecticidal activities of zizyphus jujube. Pak. J. Pharm. Sci., 24(4), 489-493 (2011).
  26. Ghaly, I.S., Said, A. and Abdel-Wahhlab, M.A. Zizyphus jujube and origanum majorana extracts protect against hydroquinone-induced clastogenicity. Environ. Toxicol. Pharmacol. 25(1), 10-19 (2008). https://doi.org/10.1016/j.etap.2007.07.002