미생물학회지 (Korean Journal of Microbiology)

  • 제48권2호
  • /
  • Pages.171-174
  • /
  • 2012
  • /
  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지
DOI QR코드

DOI QR Code

분열효모에서 sphpr1 유전자의 결실이 생장 및 mRNA Export에 미치는 영향

Effects of the Repression of sphpr1 Expression on Growth and mRNA Export in Fission Yeast

  • 이현주 (성신여자대학교 생명과학.화학부 및 기초과학연구소) ;
  • 윤진호 (성신여자대학교 생명과학.화학부 및 기초과학연구소)
  • Lee, Hyun-Joo (Basic Science Research Institute, School of Biological Science and Chemistry, Sungshin Women's University) ;
  • Yoon, Jin-Ho (Basic Science Research Institute, School of Biological Science and Chemistry, Sungshin Women's University)
  • 투고 : 2012.06.11
  • 심사 : 2012.06.20
  • 발행 : 2012.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

THOC1/Hpr1는 mRNA가 전사되는 동안 mRNP의 포장과 mRNA 방출에 관여하는 진화적으로 잘 보존된 THO 복합체의 한 소단위이다. 분열효모 Schizosaccharomyces pombe에서도 THOC1/Hpr1과 유사한 단백질을 암호화하는 유전자(sphpr1로 명명)를 찾아 그 특성을 조사하였다. 이배체 S. pombe 균주에 하나의 sphpr1 유전자만을 결실시킨 후 4분체 분석을 수행한 결과, 이 유전자는 생장에 필수적이었다. 티아민에 의해 발현이 조절되는 강력한 nmt1 프러모터를 이용하여 sphpr1를 과발현시키더라도 세포의 생장과 mRNA 방출에는 전혀 영향이 없었다. 하지만, sphpr1의 발현을 억제하면 생장이 억제되었으며 poly$(A)^+$ RNA가 핵 안에 축적되었다. 이와 같은 결과들은 sphpr1 유전자가 생장과 mRNA의 핵에서 세포질로의 방출에 관여하고 있음을 시사한다.

THOC1/Hpr1 is one subunit of THO complex that is an evolutionally conserved assembly involved in the mRNP packaging and mRNA export during transcription elongation. In fission yeast Schizosaccharomyces pombe, an ortholog (spHpr1) of THOC1/Hpr1 was identified based on sequence alignment. A deletion mutant in a diploid strain was constructed by replacing one of spHpr1-coding region with a $kan^r$ gene using one-step gene disruption method. Tetrad analysis showed that the sphpr1 is essential for growth. Over-expression of sphpr1 from strong nmt1 promoter caused no defects of growth and mRNA export. However, repression of the sphpr1 expression resulted in growth inhibition accompanied by accumulation of poly$(A)^+$ RNA in the nucleus. These results suggest that spHpr1 is involved in mRNA export from the nucleus to cytoplasm.

키워드

참고문헌

  1. Alfa, C., Fantes, P., Hyams, J., Mcleod, M., and Warbrick, E. 1993. Experiments with Fission Yeast. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., USA.
  2. Chanarat, S., Seizl, M., and Strasser, K. 2011. The Prp19 complex is a novel transcription elongation factor required for TREX occupancy at transcribed genes. Genes Dev. 25, 1147-1158. https://doi.org/10.1101/gad.623411
  3. Chavez, S. and Aguilera, A. 1997. The yeast HPR1 gene has a functional role in transcriptional elongation that uncovers a novel source of genome instability. Genes Dev. 11, 3459-3470. https://doi.org/10.1101/gad.11.24.3459
  4. Chavez, S., Beilharz, T., Rondón, A.G., Erdjument-Bromage, H., Tempst, P., Svejstrup, J.Q., Lithgow, T., and Aguilera, A. 2000. A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae. EMBO J. 19, 5824-5834. https://doi.org/10.1093/emboj/19.21.5824
  5. Fasken, M.B. and Corbett, A.H. 2009. Mechanisms of nuclear mRNA quality control. RNA Biol. 6, 237-241. https://doi.org/10.4161/rna.6.3.8330
  6. Forsburg, S.L. 1993. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21, 2955-2956. https://doi.org/10.1093/nar/21.12.2955
  7. Hurt, E., Luo, M.J., Röther, S., Reed, R., and Strässer, K. 2004. Cotranscriptional recruitment of the serine-arginine-rich (SR)-like proteins Gbp2 and Hrb1 to nascent mRNA via the TREX complex. Proc. Natl. Acad. Sci. USA 101, 1858-1862. https://doi.org/10.1073/pnas.0308663100
  8. Jimeno, S., Rondón, A.G., Luna, R., and Aguilera, A. 2002. The yeast THO complex and mRNA export factors link RNA metabolism with transcription and genome instability. EMBO J. 21, 3526-3355. https://doi.org/10.1093/emboj/cdf335
  9. Kelly, S.M. and Corbett, A.H. 2009. Messenger RNA export from the nucleus: a series of molecular wardrobe changes. Traffic 10, 1199-1208. https://doi.org/10.1111/j.1600-0854.2009.00944.x
  10. Kohler, A. and Hurt, E. 2007. Exporting RNA from the nucleus to the cytoplasm. Nat. Rev. Mol. Cell Biol. 8, 761-773. https://doi.org/10.1038/nrm2255
  11. Libri, D., Dower, K., Boulay, J., Thomsen, R., Rosbash, M., and Jensen, T.H. 2002. Interactions between mRNA export commitment, 3'-end quality control, and nuclear degradation. Mol. Cell. Biol. 22, 8254-8266. https://doi.org/10.1128/MCB.22.23.8254-8266.2002
  12. Maundrell, K. 1993. Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene 123, 127-130. https://doi.org/10.1016/0378-1119(93)90551-D
  13. Moreno, S., Klar, A., and Nurse, P. 1991. Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol. 194, 795-823. https://doi.org/10.1016/0076-6879(91)94059-L
  14. Schmid, M. and Jensen, T.H. 2010. Nuclear quality control of RNA polymerase II transcripts. Wiley Interdiscip. Rev. RNA 1, 474-485. https://doi.org/10.1002/wrna.24
  15. Stewart, M. 2010. Nuclear export of mRNA. Trends Biochem. Sci. 35, 609-617. https://doi.org/10.1016/j.tibs.2010.07.001
  16. Strasser, K., Masuda, S., Mason, P., Pfannstiel, J., Oppizzi, M., Rodriguez-Navarro, S., Rondon, A.G., Aguilera, A., Struhl, K., Reed, R., and Hurt, E. 2002. TREX is a conserved complex coupling transcription with messenger RNA export. Nature 417, 304-308. https://doi.org/10.1038/nature746
  17. Yoon, J.H., Love, D., Guhathakurta, A., Hanover, J.A., and Dhar, R. 2000. Mex67p of Schizosaccharomyces pombe interacts with Rae1p in mediating mRNA export. Mol. Cell. Biol. 20, 8767-8782. https://doi.org/10.1128/MCB.20.23.8767-8782.2000
  18. Zenklusen, D., Vinciguerra, P., Wyss, J.C., and Stutz, F. 2002. Stable mRNP formation and export require cotranscriptional recruitment of the mRNA export factors Yra1p and Sub2p by Hpr1p. Mol. Cell. Biol. 22, 8241-8253. https://doi.org/10.1128/MCB.22.23.8241-8253.2002

피인용 문헌

  1. Effects of fission yeast ortholog of THOC5 on growth and mRNA export in fission yeast vol.51, pp.4, 2015, https://doi.org/10.7845/kjm.2015.5058
  2. Effects of Tho2, a component of THO complex, on growth and mRNA export in fission yeast vol.51, pp.2, 2015, https://doi.org/10.7845/kjm.2015.5020
  3. 분열효모에서 THO 복합체의 구성요소인 Tex1/THOC3가 생장 및 mRNA 방출에 미치는 영향 vol.53, pp.4, 2012, https://doi.org/10.7845/kjm.2017.7091
  4. 분열효모에서 hnRNP-유사 단백질인 THO4가 생장 및 mRNA 방출에 미치는 영향 vol.54, pp.2, 2012, https://doi.org/10.7845/kjm.2018.8028