Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Analysis of a Region Required for the Functions of Fission Yeast Nucleoporin Nup184 and Its SUMO Modification

분열효모 핵공단백질인 Nup184의 기능에 필요한 부위 분석 및 SUMO 변성

  • Chai, Ai-Ree (Basic Science Research Institute, School of Biological Science and Chemistry, College of Natural Sciences, Sungshin Women's University) ;
  • Jang, Soo-Yeon (Basic Science Research Institute, School of Biological Science and Chemistry, College of Natural Sciences, Sungshin Women's University) ;
  • Yoon, Jin-Ho (Basic Science Research Institute, School of Biological Science and Chemistry, College of Natural Sciences, Sungshin Women's University)
  • 채애리 (성신여자대학교 생명과학.화학부 및 기초과학연구소) ;
  • 장수연 (성신여자대학교 생명과학.화학부 및 기초과학연구소) ;
  • 윤진호 (성신여자대학교 생명과학.화학부 및 기초과학연구소)
  • Received : 2012.05.17
  • Accepted : 2012.06.04
  • Published : 2012.06.30
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Abstract

The Nup188 protein is one of the largest evolutionally conserved nucleoprins (Nups) that compose the inner ring of nuclear pore complex (NPC). The Nup184 protein, fission yeast Schizosaccharomyces pombe ortholog of Nup188p, is required for normal growth and mRNA export in nutrient-rich medium (YES). Here, we identified a carboxyl region (482 to 1628) of Nup184 protein that was enough to complement the defects of both growth and mRNA export when the ${\Delta}nup184$ knock-out mutant was grown in YES medium. This region is also required for localization of GFP-Nup184 fusion to the nuclear periphery. In addition, we found that ORF of Nup184 (predicted 1564 amino-acid protein) registered in S. pombe GeneDB (hosted by Sanger Institute, UK) is 64 amino-acid residues shorter than that predicted by our sequence data. This carboxy-terminal region is necessary for the functions of Nup184p. We further demonstrated that Nup184 protein was conjugated with SUMO in vivo.

Nup188 단백질은 진화적으로 보존된 가장 큰 핵공단백질 중의 하나로 핵공복합체의 inner ring을 구성하는 인자이다. Nup188의 이종상동체인 분열효모 S. pombe의 Nup184 단백질은 영양분이 풍부한 완전배지(YES 배지)에서 정상적인 생장과 mRNA의 핵에서 세포질로의 이동에 필요하다. 본 연구에서는 ${\Delta}nup184$ 결실돌연변이를 YES 배지에서 배양할 때 보이는 생장지체와 mRNA export 결함을 상보하기 위해서 Nup184의 카르복시 부위(아미노산 잔기482에서 1628까지)가 필요함을 알아내었다. 또한 이 부위는 GFP-Nup184 융합단백질이 핵막에 위치하기 위해서도 필요하였다. 이 과정에서 S. pombe GeneDB (Sanger 연구소, 영국)에 등록되어 있는 Nup184의 열린읽기틀 (1564개의 아미노산 잔기로 된 단백질로 예측)이 우리가 얻은 염기서열 데이터에 비해 66개의 아미노산 잔기가 짧다는 것을 발견하였다. 이 카르복시-말단 부위는 Nup184의 기능에 반드시 필요하였다. 이외에도 Nup184 단백질이 세포 안에서 SUMO 변형되어 있음을 보였다.

Keywords

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