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Inhibitory Effect of Melanogenesis by 5-Pentyl-2-Furaldehyde Isolated from Clitocybe sp.

  • Kim, Young-Hee (Korea Research Institute of Bioscience and Biotechnology) ;
  • Choo, Soo-Jin (Korea Research Institute of Bioscience and Biotechnology) ;
  • Ryoo, In-Ja (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Bo-Yeon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn, Jong-Seog (Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoo, Ick-Dong (Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2012.01.31
  • Accepted : 2012.02.29
  • Published : 2012.06.28

Abstract

In the continued search for melanogenesis inhibitors from microbial metabolites, we found that the culture broth of Clitocybe sp. MKACC 53267 inhibited melanogenesis in B16F10 melanoma cells. The active component was purified by solvent extraction, silica gel chromatography, Sephadex LH-20 column chromatography, and finally by preparative HPLC. Its structure was determined as 5-pentyl-2-furaldehyde on the basis of the UV, NMR, and MS spectroscopic analysis. The 5-pentyl-2-furaldehyde potently inhibited melanogenesis in B16F10 cells with an $IC_{50}$ value of 8.4 ${\mu}g/ml$, without cytotoxicity.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea

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