Journal of Microbiology and Biotechnology

  • 제22권5호
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  • Pages.659-667
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  • 2012
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Identification of Factors Regulating Escherichia coli 2,3-Butanediol Production by Continuous Culture and Metabolic Flux Analysis

  • Lu, Mingshou (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Lee, Soo-Jin (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Kim, Bo-Rim (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Park, Chang-Hun (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Oh, Min-Kyu (Department of Chemical and Biological Engineering, Korea University) ;
  • Park, Kyung-Moon (Department of Biological and Chemical Engineering, Hongik University) ;
  • Lee, Sang-Yup (College of Science and Bioengineering, KAIST) ;
  • Lee, Jin-Won (Department of Chemical and Biomolecular Engineering, Sogang University)
  • 투고 : 2011.12.08
  • 심사 : 2012.01.14
  • 발행 : 2012.05.28
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초록

2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia coli is often used for the production of organic compounds, the wild-type E. coli does not contain two essential genes in the 2,3-BDO biosynthesis pathway, and cannot ferment 2,3-BDO. Therefore, a 2,3-BDO biosynthesis mutant strain of Escherichia coli was constructed and cultured. To determine the optimum culture factors for 2,3-BDO production, experiments were conducted under different culture environments ranging from strongly acidic to neutral pH. The extracellular metabolite profiles were obtained using high-performance liquid chromatography (HPLC), and the intracellular metabolite profiles were analyzed by ultra-performance liquid chromatography and quadruple time-of-flight mass spectrometry (UPLC/Q-TOF-MS). Metabolic flux analysis (MFA) was used to integrate these profiles. The metabolite profiles showed that 2,3-BDO production favors an acidic environment (pH 5), whereas cell mass favors a neutral environment. Furthermore, when the pH of the culture fell below 5, both the cell growth and 2,3-BDO production were inhibited.

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참고문헌

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  1. Systematic Applications of Metabolomics in Metabolic Engineering vol.2, pp.4, 2012, https://doi.org/10.3390/metabo2041090
  2. Increased 2,3-butanediol production by changing codon usages inEscherichia coli : Increased 2,3-Butanediol Production vol.61, pp.5, 2014, https://doi.org/10.1002/bab.1216
  3. Biotechnologie von Morgen: metabolisch optimierte Zellen für die bio‐basierte Produktion von Chemikalien und Treibstoffen, Materialien und Gesundheitsprodukten vol.127, pp.11, 2012, https://doi.org/10.1002/ange.201409033
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