DOI QR코드

DOI QR Code

Evaluation of a Pretreatment Method for Two-Dimensional Gel Electrophoresis of Synovial Fluid Using Cartilage Oligomeric Matrix Protein as a Marker

  • Kong, Min-Kyung (Department of Molecular Science and Technology, Ajou University) ;
  • Min, Byoung-Hyun (Department of Molecular Science and Technology, Ajou University) ;
  • Lee, Pyung-Cheon (Department of Molecular Science and Technology, Ajou University)
  • 투고 : 2011.11.02
  • 심사 : 2012.01.02
  • 발행 : 2012.05.28

초록

Osteoarthritis (OA) is the most common rheumatic pathology. One of the major objectives of OA research is the development of early diagnostic strategies such as those using proteomic technology. Synovial fluid (SF) in OA patients is a potential source of biomarkers for OA. The efficient and reliable preparation of SF proteomes is a critical step towards biomarker discovery. In this study, we have optimized a pretreatment method for twodimensional gel electrophoresis (2DE) separation of the SF proteome, by enriching low-abundance proteins and simultaneously removing hyaluronic acid, albumin, and IgG. SF samples pretreated using this optimized method were then evaluated by 1DE and 2DE separation followed by immunodetection of cartilage oligomeric matrix protein (COMP), a known OA biomarker, and by the identification of 3 proteins (apolipoprotein, haptoglobin precursor, and fibrinogen D fragment) that are related to joint diseases.

키워드

참고문헌

  1. Chang, X., J. Han, Y. Zhao, X. Yan, S. Sun, and Y. Cui. 2010. Increased expression of carbonic anhydrase I in the synovium of patients with ankylosing spondylitis. BMC Musculoskelet. Disord. 11: 279. https://doi.org/10.1186/1471-2474-11-279
  2. De Ceuninck, F. and F. Berenbaum. 2009. Proteomics: Addressing the challenges of osteoarthritis. Drug Discov. Today 14: 661-667. https://doi.org/10.1016/j.drudis.2009.05.006
  3. Ghosh, P. 1994. The role of hyaluronic acid (hyaluronan) in health and disease: Interactions with cells, cartilage and components of synovial fluid. Clin. Exp. Rheumat. 12: 75-82.
  4. Gibson, D. S. and M. E. Rooney. 2007. The human synovial fluid proteome: A key factor in the pathology of joint disease. Proteomics Clin. Appl. 1: 889-899. https://doi.org/10.1002/prca.200700044
  5. Kamphorst, J. J., R. Van Der Heijden, J. DeGroot, F. P. J. G. Lafeber, T. H. Reijmers, B. Van El, et al. 2007. Profiling of endogenous peptides in human synovial fluid by NanoLC-MS: Method validation and peptide identification. J. Proteome Res. 6: 4388-4396. https://doi.org/10.1021/pr0704534
  6. Liao, H., J. Wu, E. Kuhn, W. Chin, B. Chang, M. D. Jones, et al. 2004. Use of mass spectrometry to identify protein biomarkers of disease severity in the synovial fluid and serum of patients with rheumatoid arthritis. Arthritis Rheum. 50: 3792-3803. https://doi.org/10.1002/art.20720
  7. Marshall, K. W., H. Zhang, and N. Nossova. 2006. Chondrocyte genomics: Implications for disease modification in osteoarthritis. Drug Discov. Today 11: 825-832. https://doi.org/10.1016/j.drudis.2006.07.004
  8. Morozzi, G., M. Fabbroni, F. Bellisai, G. Pucci, and M. Galeazzi. 2007. Cartilage oligomeric matrix protein level in rheumatic diseases: Potential use as a marker for measuring articular cartilage damage and/or the therapeutic efficacy of treatments. N.Y. Acad. Sci. 1108: 398-407. https://doi.org/10.1196/annals.1422.041
  9. Patra, D. and L. J. Sandell. 2011. Recent advances in biomarkers in osteoarthritis. Curr. Opin. Rheumatol.
  10. Ruiz-Romero, C. and F. J. Blanco. 2010. Proteomics role in the search for improved diagnosis, prognosis and treatment of osteoarthritis. Osteoarthr. Cartil. 18: 500-509. https://doi.org/10.1016/j.joca.2009.11.012
  11. Ruiz-Romero, C., V. Calamia, V. Carreira, J. Mateos, P. Fernandez, and F. J. Blanco. 2010. Strategies to optimize two-dimensional gel electrophoresis analysis of the human joint proteome. Talanta 80: 1552-1560. https://doi.org/10.1016/j.talanta.2009.05.022
  12. Sinz, A., M. Bantscheff, S. Mikkat, B. Ringel, S. Drynda, J. Kekow, et al. 2002. Mass spectrometric proteome analyses of synovial fluids and plasmas from patients suffering from rheumatoid arthritis and comparison to reactive arthritis or osteoarthritis. Electrophoresis 23: 3445-3456. https://doi.org/10.1002/1522-2683(200210)23:19<3445::AID-ELPS3445>3.0.CO;2-J
  13. Skriner, K., K. Adolph, P. R. Jungblut, and G. R. Burmester. 2006. Association of citrullinated proteins with synovial exosomes. Arthritis Rheumat. 54: 3809-3814. https://doi.org/10.1002/art.22276
  14. Tseng, S., A. H. Reddi, and P. E. Di Cesare. 2009. Cartilage oligomeric matrix protein (COMP): A biomarker of arthritis. Biomark. Insights 2009: 33-44.
  15. Williams, A., J. R. Smith, D. Allaway, P. Harris, S. Liddell, and A. Mobasheri. 2011. Applications of proteomics in cartilage biology and osteoarthritis research. Front. Biosci. 16: 2622-2641. https://doi.org/10.2741/3876
  16. Xiang, Y., T. Sekine, H. Nakamura, S. Imajoh-Ohmi, H. Fukuda, K. Nishioka, and T. Kato. 2004. Proteomic surveillance of autoimmunity in osteoarthritis: Identification of triosephosphate isomerase as an autoantigen in patients with osteoarthritis. Arthritis Rheumat. 50: 1511-1521. https://doi.org/10.1002/art.20189
  17. Yamagiwa, H., G. Sarkar, M. C. Charlesworth, D. J. McCormick, and M. E. Bolander. 2003. Two-dimensional gel electrophoresis of synovial fluid: Method for detecting candidate protein markers for osteoarthritis. J. Orthopaed. Sci. 8: 482-490. https://doi.org/10.1007/s00776-003-0657-3

피인용 문헌

  1. iTRAQ-based proteomics reveals novel biomarkers of osteoarthritis vol.18, pp.7, 2012, https://doi.org/10.3109/1354750x.2013.810667
  2. A Review of the Pathogenesis of Osteoarthritis and the Use of Intra-articular Platelet Therapy for Joint Disease in Animals and Humans vol.5, pp.None, 2012, https://doi.org/10.4137/btri.s14578
  3. A Composite Dermal Filler Comprising Cross-Linked Hyaluronic Acid and Human Collagen for Tissue Reconstruction vol.25, pp.3, 2012, https://doi.org/10.4014/jmb.1411.11029
  4. Effects of ultrasound therapy on the synovial fluid proteome in a rabbit surgery-induced model of knee osteoarthritis vol.18, pp.None, 2012, https://doi.org/10.1186/s12938-019-0637-2
  5. Biomarkers of Joint Damage in Osteoarthritis: Current Status and Future Directions vol.2021, pp.None, 2021, https://doi.org/10.1155/2021/5574582