Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biochemical Characterization of Thermophilic Dextranase from a Thermophilic Bacterium, Thermoanaerobacter pseudethanolicus

  • Park, Tae-Soon (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Jeong, Hyung-Jae (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ko, Jin-A (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ryu, Young-Bae (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Park, Su-Jin (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Do-Man (School of Biological Sciences and Technology and the Research Institute for Catalysis, Chonnam National University) ;
  • Kim, Young-Min (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Woo-Song (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Received : 2011.12.12
  • Accepted : 2012.01.17
  • Published : 2012.05.28
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Abstract

TPDex, a putative dextranase from Thermoanaerobacter pseudethanolicus, was purified as a single 70 kDa band of 7.37 U/mg. Its optimum pH was 5.2 and the enzyme was stable between pH 3.1 and 8.5 at $70^{\circ}C$. A half-life comparison showed that TPDex was stable for 7.4 h at $70^{\circ}C$, whereas Chaetominum dextranase (CEDex), currently used as a dextranase for sugar milling, was stable at $55^{\circ}C$. TPDex showed broad dextranase activity regardless of dextran types, including dextran T2000, 742CB dextran, and alternan. TPDex showed the highest thermostability among the characterized dextranases, and may be a suitable enzyme for use in sugar manufacture without decreased temperature.

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