Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Growth Response of Avena sativa in Amino-Acids-Rich Soils Converted from Phenol-Contaminated Soils by Corynebacterium glutamicum

  • Lee, Soo-Youn (Department of Bioprocess Engineering, Chonbuk National University) ;
  • Kim, Bit-Na (Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University) ;
  • Choi, Yong-Woo (Fuel Cell Research Center, Korea Institute of Energy Research) ;
  • Yoo, Kye-Sang (Department of Chemical Engineering, Seoul National University of Science and Technology) ;
  • Kim, Yang-Hoon (Department of Microbiology, Chungbuk National University) ;
  • Min, Ji-Ho (Department of Bioprocess Engineering, Chonbuk National University)
  • Received : 2011.08.31
  • Accepted : 2011.12.02
  • Published : 2012.04.28
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Abstract

The biodegradation of phenol in laboratory-contaminated soil was investigated using the Gram-positive soil bacterium Corynebacterium glutamicum. This study showed that the phenol degradation caused by C. glutamicum was greatly enhanced by the addition of 1% yeast extract. From the toxicity test using Daphnia magna, the soil did not exhibit any hazardous effects after the phenol was removed using C. glutamicum. Additionally, the treatment of the phenol-contaminated soils with C. glutamicum increased various soil amino acid compositions, such as glycine, threonine, isoleucine, alanine, valine, leucine, tyrosine, and phenylalanine. This phenomenon induced an increase in the seed germination rate and the root elongation of Avena sativa (oat). This probably reflects that increased soil amino acid composition due to C. glutamicum treatment strengthens the plant roots. Therefore, the phenol-contaminated soil was effectively converted through increased soil amino acid composition, and additionally, the phenol in the soil environment was biodegraded by C. glutamicum.

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References

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