Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Identification of the Fur-Binding Site in Regulatory Region of the Vulnibactin-Receptor Gene in Vibrio vulnificus

  • Lee, Hyun-Jung (Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health) ;
  • Lee, Kyu-Ho (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
  • Received : 2011.08.17
  • Accepted : 2011.09.19
  • Published : 2012.01.28
Download PDF
⟨ Previous Next ⟩

Abstract

The Vibrio vulnificus vuuA gene, of which expression is repressed by a complex of iron and ferric uptake regulator (Fur), was characterized to localize the Fur-binding site in its upstream regulatory region. In silico analysis suggested the presence of two possible Fur-binding sites; one is a classical Fur-box and the other is a previously reported distinct Fur-binding site. Site-directed mutagenesis and DNase I protection assays revealed the binding site for the iron-Fur complex, which includes an extended inverted repeat containing a homologous sequence to the classical Fur-box.

Keywords

References

  1. Butterton, J. R. and S. B. Calderwood. 1994. Identification, cloning, and sequencing of a gene required for ferric vibriobactin utilization by Vibrio cholerae. J. Bacteriol. 176: 5631-5638. https://doi.org/10.1128/jb.176.18.5631-5638.1994
  2. Chen, C. Y., K. M. Wu, Y. C. Chang, C. H. Chang, H. C. Tsai, T. L. Liao, et al. 2003. Comparative genome analysis of Vibrio vulnificus, a marine pathogen. Genome Res. 13: 2577-2587. https://doi.org/10.1101/gr.1295503
  3. Delany, I., G. Spohn, A. F. Pacheco, R. Ieva, C. Alaimo, R. Rappuoli, and V. Scarlato. 2002. Autoregulation of Helicobacter pylori Fur revealed by functional analysis of the iron-binding site. Mol. Microbiol. 46: 1107-1122. https://doi.org/10.1046/j.1365-2958.2002.03227.x
  4. Escolar, L., J. Perez-Martin, and V. de Lorenzo. 1999. Opening the iron box: Transcriptional metalloregulation by the Fur protein. J. Bacteriol. 181: 6223-6229.
  5. Henderson, D. P. and S. M. Payne. 1994. Vibrio cholerae iron transport systems: Roles of heme and siderophore iron transport in virulence and identification of a gene associated with multiple iron transport systems. Infect. Immun. 62: 5120-5125.
  6. Gulig, P. A., K. L. Bourdage, and A. M. Starks. 2005. Molecular pathogenesis of Vibrio vulnificus. J. Microbiol. 43: 118-131.
  7. Grifliths, G. L., S. P. Sigel, S. M. Payne, and J. B. Neilands. 1984. Vibriobactin, a siderophore from Vibrio cholerae. J. Biol. Chem. 259: 383-385.
  8. Kim, Y. R., S. E. Lee, C. M. Kim, S. Y. Kim, E. K. Shin, D. H. Shin, et al. 2003. Characterization and pathogenic significance of Vibrio vulnificus antigens preferentially expressed in septicemic patients. Infect. Immun. 71: 5461-5471. https://doi.org/10.1128/IAI.71.10.5461-5471.2003
  9. Lee, H.-J., K.-J. Park, A. Y. Lee, S. G. Park, B. C. Park, K.-H. Lee, and S.-J. Park. 2003. Regulation of fur expression by RpoS and Fur in Vibrio vulnificus. J. Bacteriol. 185: 5891-5896. https://doi.org/10.1128/JB.185.19.5891-5896.2003
  10. Lee, H.-J., S. H. Bang, K.-H. Lee, and S.-J. Park. 2007. Positive regulation of fur gene expression via direct interaction of Fur in a pathogenic bacterium, Vibrio vulnificus. J. Bacteriol. 189: 2629-2636. https://doi.org/10.1128/JB.01791-06
  11. Litwin, C. M., T. W. Rayback, and J. Skinner. 1996. Role of catechol siderophore synthesis in Vibrio vulnificus virulence. Infect. Immun. 64: 2834-2838.
  12. Miyamotoa, K., K. Kosakaia, S. Ikebayashia, T. Tsuchiyaa, S. Yamamotob, and H. Tsujibo. 2009. Proteomic analysis of Vibrio vulnificus M2799 grown under iron-repleted and iron-depleted conditions. Microb. Pathog. 46: 171-177. https://doi.org/10.1016/j.micpath.2008.12.004
  13. Okujo, N., M. Saito, S. Yamamoto, T. Yoshida, S. Miyoshi, and S. Shinoda. 1994. Structure of vulnibactin, a new polyaminecontaining siderophore from Vibrio vulnificus. Biometals 7: 109-116.
  14. Park, J. H., Y. J. Cho, J. K. Lee, J. Chun, K. S. Kim, K.-H. Lee, et al. 2011. Complete genome sequence of Vibrio vulnificus MO6-24/O. J. Bacteriol. 193: 2062-2063. https://doi.org/10.1128/JB.00110-11
  15. Stoebner, J. A. and S. M. Payne, 1988. Iron-regulated hemolysin production and utilization of heme and hemoglobin by Vibrio cholera. Infect. Immun. 56: 2891-2895.
  16. Strom, M. S. and R. N. Paranjpye. 2000. Epidemiology and pathogenesis of Vibrio vulnificus. Microbes Infect. 2: 177-188. https://doi.org/10.1016/S1286-4579(00)00270-7
  17. Webster, A. C. D. and C. M. Litwin. 2000. Cloning and characterization of vuuA, a gene encoding the Vibrio vulnificus ferric vulnibactin receptor. Infect. Immun. 68: 526-534. https://doi.org/10.1128/IAI.68.2.526-534.2000
  18. Wright, A. C., J. G. Morris Jr., D. R. Maneval Jr., K. Richardson, and J. B. Kaper. 1985. Cloning of the cytotoxinhemolysin gene of Vibrio vulnificus. Infect. Immun. 50: 922-924.

Cited by

  1. Regulation of haemolysin (VvhA) production by ferric uptake regulator (Fur) in Vibrio vulnificus: repression of vvhA transcription by Fur and proteolysis of VvhA by Fur‐repressive exoproteases vol.88, pp.4, 2013, https://doi.org/10.1111/mmi.12224
  2. Multiple High-affinity Iron-uptake Systems of Vibrio vulnificus vol.43, pp.3, 2013, https://doi.org/10.4167/jbv.2013.43.3.168
  3. Vibrio vulnificus Secretes an Insulin-degrading Enzyme That Promotes Bacterial Proliferation in Vivo vol.290, pp.30, 2012, https://doi.org/10.1074/jbc.m115.656306
  4. Regulation of iron-uptake systems in Vibrio vulnificus, a ferrophilic bacterium vol.4, pp.2, 2012, https://doi.org/10.30579/mbse.2021.4.2.69