Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Elastic Liposome Formulation for Transdermal Delivery of Rutin

루틴의 피부 흡수 증진을 위한 탄성 리포좀 제형 연구

  • Lim, Myoung-Sun (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Han, Seat-Byeol (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Kwon, Soon-Sik (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Park, Min-A (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Park, Soo-Nam (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology)
  • 임명선 (서울과학기술대학교 정밀화학과, 나노바이오화장품 연구실 화장품종합기술연구소) ;
  • 한샛별 (서울과학기술대학교 정밀화학과, 나노바이오화장품 연구실 화장품종합기술연구소) ;
  • 권순식 (서울과학기술대학교 정밀화학과, 나노바이오화장품 연구실 화장품종합기술연구소) ;
  • 박민아 (서울과학기술대학교 정밀화학과, 나노바이오화장품 연구실 화장품종합기술연구소) ;
  • 박수남 (서울과학기술대학교 정밀화학과, 나노바이오화장품 연구실 화장품종합기술연구소)
  • Received : 2012.03.15
  • Accepted : 2012.06.11
  • Published : 2012.06.30
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Abstract

In this study, we prepared elastic liposome containing rutin, known as antioxidants, and evaluated the physical characterization and enhanced skin permeation effect. The elastic liposome was prepared using the different ratios of egg phospholipids and $Tego^{(R)}$ care 450. The mean diameter of rutin loaded elastic liposomes formulations ranged between 205.7 ~ 298.0 nm and deforability 20.9 ~ 42.5, The loading efficiency was observed to be 52.0 ~ 71.0 %. The highest loading efficiency (71.0 %) and deformability (42.5) were observed at the optimal ratio of 85 : 15 (egg phospholipids : $Tego^{(R)}$ care 450) in the 0.1 % rutin loaded elastic liposome formulations. The elastic liposome formulation was selected for further transdermal permeation study. The elastic liposome(129.9 ${\mu}g/cm^2$) exhibited a significantly higher skin permeation compared with general liposome (98.0 ${\mu}g/cm^2$) and 1,3-butylene glycol (76.3 ${\mu}g/cm^2$) solution. These results suggest that the elastic liposome formulation using $Tego^{(R)}$ care 450 as a major edge activator could be useful for the delivery of active ingredient through the skin barrier.

본 연구에서는 항산화 활성이 우수한 루틴을 담지한 탄성 리포좀을 제조하여, 이 제형의 물리적 특성과 증진된 피부 투과 효과를 평가하였다. 탄성 리포좀은 인지질과 계면활성제의 비율을 달리하여 제조하였다. 루틴을 담지한 탄성리포좀의 평균 입자 크기는 205.7 ~ 298.0 nm, 가변형성은 20.9 ~ 42.5, 포집효율은 52.0 ~ 71.0 %로 측정되었다. 0.1 % 루틴을 담지한 탄성 리포좀 중에서 인지질과 계면활성제 비율이 85 : 15 인 경우가 가장 높은 포집효율(71.0 %)과 가변형성 지수(42.5)를 나타내었다. 이 제형을 대상으로 피부 투과 실험을 진행하였다. 그 결과 대조군으로 사용된 일반 리포좀(98.0 ${\mu}g/cm^2$)과 1,3-butylene glycol (76.3 ${\mu} g/cm^2$) 용액보다 탄성 리포좀의 피부 투과능(129.9 ${\mu}g/cm^2$)이 훨씬 더 크게 나타났다. 이러한 결과들로 미루어 보아 $Tego^{(R)}$ care 450을 이용한 탄성 리포좀이 피부를 통한 유효성분 전달에 유용하게 이용될 수 있음을 확인하였다.

Keywords

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