Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Free radical scavenging effect and protective activity from oxidative stress of broccoli flowers and sprouts

Broccoli flower와 Broccoli sprout의 라디칼 소거능 및 산화적 스트레스 개선 효과

  • Kim, Hyun-Young (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University) ;
  • Lee, Young-A (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Cho, Eun-Ju (Department of Food Science, Gyeongnam National University of Science and Technology)
  • 김현영 (경남과학기술대학교 식품과학부) ;
  • 이영아 (부산대학교 식품영양학과 및 노인생활환경연구소) ;
  • 조은주 (부산대학교 식품영양학과 및 노인생활환경연구소)
  • Received : 2012.01.25
  • Accepted : 2012.03.23
  • Published : 2012.03.31
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Abstract

In this study, the antioxidative effect and protective potential against oxidative damage of extract and fractions from broccoli were investigated under in vitro and cellular system. The methanol (MeOH) extracts of broccoli flowers and sprouts were partitioned as dichloromethane, n-butanol (BuOH) and aqueous fractions. The comparison of antioxidative effect of broccoli flowers and sprouts showed that broccoli sprouts exerted the more effective protective activity from 2,2'-azobis (2-aminopropane) dihydrochloride (AAPH)-induced oxidative stress in LLC-$PK_1$ porcine renal epithelial cell. In addition, the extract and fractions from broccoli sprouts showed strong scavenging effect of 1,1-diphenyl-2-picrylhydrazyl radical and the BuOH fraction exerted the strongest activity. Therefore, the BuOH fraction was evaluated as the most active fraction with strong radical scavenging activity among the fractions of broccoli flowers and sprouts. The present study suggests the antioxidative potential against free radical-induced oxidative damage of flowers and sprouts of broccoli. In addition, the BuOH fraction of broccoli is considered as the active fraction with antioxidative effect.

Keywords

References

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