Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Molecular cloning of Prophenoloxidase (PPO) gene related to melanin formation of elytra of Harmonia axyridis

무당벌레(Harmonia axyridis) 초시색상 패턴의 유전 및 이의 관련유전자 탐색

  • Kim, Sae-Hee (Dept. Applied Biology, Chungnam National University) ;
  • Seo, Mi-Ja (Dept. Applied Biology, Chungnam National University) ;
  • Park, Min-Woo (Dept. Applied Biology, Chungnam National University) ;
  • Yu, Yong-Man (Dept. Applied Biology, Chungnam National University) ;
  • Youn, Young-Nam (Dept. Applied Biology, Chungnam National University)
  • 김세희 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 서미자 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 박민우 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 유용만 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 윤영남 (충남대학교 농업생명과학대학 응용생물학과)
  • Received : 2012.02.10
  • Accepted : 2012.03.23
  • Published : 2012.03.31
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Abstract

In order to cloning of PPO gene as a melanin formation related genes involved in hardening and pigmentation of insect integument or wing, we cloned cDNA and analyzed the sequence of PPO gene of H. axyridis. PPO2 primer were designed based on the sequences of PPO genes of Tribolium castaneum and Drosophila melanogaster, and then plasmid DNA were cloned from PCR products obtained from different two color patterns. When the plasmid DNA band pattern were digested by restriction enzymes, BamH1, Xba1, and EcoR1, we found same size band pattern. However, this sequence was not homologous to sequence of T. castaneum PPO gene. Using the primer designed based on the sequence of D. melanogaster, 209 bp PCR product was observed.

Keywords

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