DOI QR코드

DOI QR Code

질소원 및 pH 조절빈도에 따른 Chlorella vulgaris의 성장특성 및 하수고도처리능 평가

Growth and N, P removal efficiency of Chlorella vulgaris according to the nitrogen sources and pH condition

  • 한수현 (경희대학교 공과대학 환경공학과) ;
  • 김선진 (경희대학교 공과대학 환경공학과) ;
  • 김태형 (경희대학교 공과대학 환경공학과) ;
  • 조기주 (경희대학교 공과대학 환경공학과) ;
  • 이윤희 (경희대학교 공과대학 환경공학과) ;
  • 황선진 (경희대학교 공과대학 환경공학과)
  • Han, Su-Hyun (Department of Environmental Science and Engineering, Kyung Hee University) ;
  • Kim, Sun-Jin (Department of Environmental Science and Engineering, Kyung Hee University) ;
  • Kim, Tae-Hyeong (Department of Environmental Science and Engineering, Kyung Hee University) ;
  • Cho, Ki-Ju (Department of Environmental Science and Engineering, Kyung Hee University) ;
  • Lee, Yunhee (Department of Environmental Science and Engineering, Kyung Hee University) ;
  • Hwang, Sun-Jin (Department of Environmental Science and Engineering, Kyung Hee University)
  • 투고 : 2012.10.23
  • 심사 : 2012.12.05
  • 발행 : 2012.12.15

초록

This study aimed to investigate growth rate and nutrient removal efficiency of Chlorella vulgaris according to nitrogen sources and frequency of pH adjustment. Nitrogen and phosphorus removal efficiencies were evaluated in the three different conditions using $NO_3{^-}$, $NH_4{^+}$ as a sole nitrogen source and mixed condition. Initial nutrient concentrations in artificial wastewater were 30 mg-N/L and 3 mg-P/L similar to secondary wastewater effluent. When nitrogen source was $NO_3{^-}$, there was no inhibition on the growth of C. vulgaris with adjusting pH every 24 hr while growth inhibition occurred with $NH_4{^+}$ caused by pH drop. N, P removal efficiencies were no significant depending on the nitrogen sources. As pH was adjusted to 7 by pH-stat, growth rate and nutrient removal efficiencies were increased compared to adjusting pH every 24 hr, however, growth rate and nutrient removal efficiencies were no significant depending on the nitrogen sources.

키워드

참고문헌

  1. Andrew D. Eaton, Lenore S. Clesceri, Eugene W. Rice, Arnold E. Greenberg (2005) 21st Edition Standard methods for the examination of water & wastewater, Centennial Edition, APHA AWWA WEF
  2. C. Pizarro, W. Mulbry, D. Blersch, P. Kangas (2006) An economic assessment of algal turf scrubber technology for treatment of dairy manure effluent, Ecol. Eng., 26, 321-327 https://doi.org/10.1016/j.ecoleng.2005.12.009
  3. Cromar NJ, Fallowfield HJ, Martin NJ (1996) Influence of environmental parameters on biomass production and nutrient removal in a high rate algal pond operated by continuous culture, Wat. Sci. Technol., 34, 133-140
  4. D. Voltolina, H. Gomez-Villa, G. Correa (2005) Nitrogen removal and recycling by Scenedesmus obliquus in semicontinuous cultures using artificial wastewater and a simulated light and temperature cycle, Bioresour. Technol., 96 (3), 359-362 https://doi.org/10.1016/j.biortech.2004.04.004
  5. Flores E, Guerrero MG, Losadh M (1980) Short term ammonium inhibition of nitrate utilization in Anacystis nidulans and other cyanobateria, Arch. Microbiol., 128, 137-140 https://doi.org/10.1007/BF00406150
  6. Hyenstrand, P., Rydin, E. & Gunnerhed, M. (2000) Response of pelagic cyanobacteria to iron additions-enclosure experiments from Lake Erken., J. Plankton Res., 22, 1113-1126 https://doi.org/10.1093/plankt/22.6.1113
  7. Khan, M., Yoshida, N. (2008) Effect of L-glutamic acid on the growth and ammonium removal from ammonium solution and natural wastewater by Chlorella vulgaris NTM06. Bioresource Technology, 99, 575-582. https://doi.org/10.1016/j.biortech.2006.12.031
  8. K. Lee, C.-G. Lee (2001) Effect of light/dark cycles on wastewater treatments by microalgae, Biotechnol. Bioprocess Eng., 6 (3), 194-199 https://doi.org/10.1007/BF02932550
  9. Martinez, M.E., Sanchez, S., Jimenez, J.M., Yousfis, F.E., Munoz, L. (2000) Nitrogen and phosphorus removal from urban wastewater by the microalga Scenedesmus obliquus, Bioresource Technology, 73 (3), 263-272 https://doi.org/10.1016/S0960-8524(99)00121-2
  10. Oswald, W. J. (2003) My sixty years in applied algology. Journal of Applied Phycology. 15, 99-106 https://doi.org/10.1023/A:1023871903434
  11. Perez-Garcia, O., Escalante, F.M.E., de-Bashan, L.E., Bashan, Y. (2011) Heterotrophic culture of microalgae: Metabolism and potential products, Water Research, 45 (1), 11-36 https://doi.org/10.1016/j.watres.2010.08.037
  12. Syrett, P. J. (1981) Nitrogen metabolism of microalgae. Physiological bases of phytoplankton ecology, Can. Bull. Fish. Aquat. Sci. 210, 182-210
  13. Xu N, Z hang X, Fan X, Han L, Zeng C (2001) Effects of nitrogen source and concentration on growth rate and fatty acid composition of Ellipsiidion sp. (Eustigmatophyta). J. Appl. Phycol., 13, 463-469 https://doi.org/10.1023/A:1012537219198
  14. Yanqun Li, Mark Horsman, Bei Wang, Nan Wu, Christopher Q. Lan (2008) Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans, Appl Microbiol Biotechnol, 81, 629-636 https://doi.org/10.1007/s00253-008-1681-1
  15. Yongmanitchai W, Ward OP (1991) Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions, Appl. Environ. Microbiol, 57 (2), 419-425
  16. Quay Dortch (1990) The interaction between ammonium and nitrate uptake in phytoplankton, MARINE ECOLOGY PROGRESS SERIES, 61, 183-201 https://doi.org/10.3354/meps061183