Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Protective Effects of Coptidis Rhizoma on Ethanol-induced Gastric Ulcer in Mice

에탄올 유발 위점막 손상에 대한 황련 물 추출물의 방어효과

  • Byun, Joon-Seok (3rd Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University)
  • 변준석 (대구한의대 한의과대학 비계내과학교실)
  • Received : 2012.01.09
  • Accepted : 2012.02.15
  • Published : 2012.02.25
Download PDF
⟨ Previous Next ⟩

Abstract

The exposure of gastric mucosa to ethanol produces acute ulcers mediated by inflammatory processes, hemorrhagic erosions and increase of reactive oxygen species. The purpose of this study was to assess the effects of Coptidis Rhizoma(CR) aqueous extracts on hydrochloride (HCl)/ethanol induced gastric ulcer in mice as compared with rebamipide (30 mg/kg) and ranitidine (100 mg/kg). Stomach ulcers were induced by oral ingestion of HCl/ethanol. CR extracts (125, 250 and 500 mg/kg) were orally administered, once a day for 7 continuous days, and 1 hr after last 7th treatment of CR extracts stomach ulcers were induced. Effects of CR extracts on HCl/ethanol-induced gastric ulcer were evaluated based on gross and microscopic observations with anti-oxidant activities. All three different dosages of CR extract significantly decreased HCl/ethanol-induced gastric ulcer compared with the HCl/ethanol control mice. CR extracts also strengthened the antioxidative defense systems - decreased the level of lipid peroxidation but increased the level of catalase, superoxide dismutase and nitrate/nitrite compared with the HCl/ethanol control. The effects of CR extract 500 mg/kg were similar to that of 30 mg/kg rebamipide, and CR extract 250 mg/kg showed similar anti-ulcer effects as compared with ranitidine 100 mg/kg. These results suggest that the gastroprotective effects of CR extracts on mice ulcer models can be attributed to its ameliorating effect on oxidative damages.

Keywords

References

  1. Alqasoumi, S., Al-Sohaibani, M., Al-Howiriny, T., Al-Yahya, M., Rafatullah, S. Rocket. salad herb with potential gastric anti-ulcer activity. World J Gastroenterol 15: 1958-1965, 2009. https://doi.org/10.3748/wjg.15.1958
  2. Mizui, T., Sato, H., Hirose, F., Doteuchi, M. Effect of antiperoxidative drugs on gastric damage induced by ethanol in rats. Life Sci. 41: 755-763, 1987. https://doi.org/10.1016/0024-3205(87)90456-5
  3. Oyagi, A., Ogawa, K., Kakino, M., Hara, H. Protective effects of a gastrointestinal agent containing Korean red ginseng on gastric ulcer models in mice. BMC Complement Altern Med. 10: 45, 2010. https://doi.org/10.1186/1472-6882-10-45
  4. Yoshida, N., Yoshikawa, T., Iinuma, S., Arai, M., Takenaka, S., Sakamoto, K., Miyajima, T., Nakamura, Y., Yagi, N., Naito, Y., Mukai, F., Kondo, M. Rebamipide protects against activation of neutrophils by Helicobacter pylori. Dig Dis Sci. 41: 1139-1144, 1996. https://doi.org/10.1007/BF02088229
  5. Grover, J.K., Adiga, G., Vats, V., Rathi, S.S. Extracts of Benincasa hispida prevent development of experimental ulcers. J Ethnopharmacol. 78: 159-164, 2001. https://doi.org/10.1016/S0378-8741(01)00334-8
  6. Ahmadi, A., Ebrahimzadeh, M.A., Ahmad-Ashrafi, S., Karami, M., Mahdavi, M.R., Saravi, S.S. Hepatoprotective, antinociceptive and antioxidant activities of cimetidine, ranitidine and famotidine as histamine H2 receptor antagonists. Fundam Clin Pharmacol. 25: 72-79, 2011. https://doi.org/10.1111/j.1472-8206.2009.00810.x
  7. 전국한의과대학 본초학교수. 본초학. 서울, 영림사, pp 220-223, 2004.
  8. Talhouk, R.S., Karam, C., Fostok, S., El-Jouniw, Barbour, E.K. Anti-inflammatory bioactivities in plant extracts. J Med Food. 10: 1-10, 2007. https://doi.org/10.1089/jmf.2005.055
  9. Xu, J.T., wang, L.Q., Xu, B., Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Research development of Coptis chinensis. 26: 704-707, 2004.
  10. Hahn, F.E., Ciak, J. Berberine. Antibiotics. 3: 577-584, 1975.
  11. Akhter, M.H., Sabir, M., Bhide, N.K. Anti-inflammatory effect of berberine in rats injected locally with cholera toxin. Indian Journal of Medical Research. 65: 133-141, 1977.
  12. Makhey, D., Gatto, B., Yu, C., Liu, A., Liu, L.F., LaVoie, E.J. Protoberberine alkaloids and related compounds as dual inhibitors of mammalian topoisomerase I and II. Medical Chemistry Research. pp 51-52, 1995.
  13. Ishihara, T., Tanaka, K., Tashiro, S., Yoshida, K., Mizushima, T. Protective effect of rebamipide against celecoxib-induced gastric mucosal cell apoptosis. Biochem Pharmacol. 79: 1622-1633, 2010. https://doi.org/10.1016/j.bcp.2010.01.030
  14. Oyagi, A., Ogawa, K., Kakino, M., Hara, H. Protective effects of a gastrointestinal agent containing Korean red ginseng on gastric ulcer models in mice. BMC Complement Altern Med. 10: 45, 2010. https://doi.org/10.1186/1472-6882-10-45
  15. Morais, T.C., Pinto, N.B., Carvalho, K.M., Rios, J.B., Ricardo, N.M., Trevisan, M.T., Rao, V.S., Santos, F.A. Protective effect of anacardic acids from cashew (Anacardium occidentale) on ethanol-induced gastric damage in mice. Chem Biol Interact. 183: 264-269, 2010. https://doi.org/10.1016/j.cbi.2009.10.008
  16. Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randall, R.J. Protein measurement with the Folin phenol reagent. J Biol Chem. 193: 265-275, 1951.
  17. Agar, E., Bosnak, M., Amanvermez, R., Demir, S., Ayyildiz, M., Celik, C. The effect of ethanol on lipid peroxidation and glutathione level in the brain stem of rat, Neuroreport. 10: 1799-1801, 1999. https://doi.org/10.1097/00001756-199906030-00032
  18. Aebi, H. Catalase. In: Bergmeyer HU (Ed.), Methods in Enzymatic Analysis. New York: Academic Press Inc. pp 673-686, 1974.
  19. Beauchamp, C., Fridovich, I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 44: 276-287, 1971. https://doi.org/10.1016/0003-2697(71)90370-8
  20. Green, L.C., Wagner, D.A., Glogowski, J., Skipper, P.L., Wishnok, J.S., Tannenbaum, C.R. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem. 10: 131-138, 1982.
  21. Ku, S.K., Seo, B.I., Park, J.H., Park, G.Y., Seo, Y.B., Kim, J.S., Lee, H.S., Roh, S.S. Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity. World J Gastroenterol. 15: 4799-4805, 2009. https://doi.org/10.3748/wjg.15.4799
  22. 김상찬, 이동언, 권영규. 順氣和中湯이 indomethacin으로 유발된 위궤양에 미치는 영향. 동의생리병리학회지 17: 326-337, 2003.
  23. Dragland, S., Senoo, H., Wake, K., Holte, K., Blomhoff, R. Several culinary and medicinal herbs are important sources of dietary antioxidants. J Nutr. 133: 1286-1290, 2003.
  24. Szabo, S., Brown, A. Prevention of ethanol-induced vascular injury and gastric mucosal lesions by sucralfate and its components: possible role of endogenous sulfhydryls. Proc Soc Exp Biol Med. 185: 493-497, 1987. https://doi.org/10.3181/00379727-185-4-RC1
  25. Lee, B.I., Nugroho, A., Bachri, M.S., Choi, J., Lee, K.R., Choi, J.S., Kim, W.B., Lee, K.T., Lee, J.D., Park, H.J. Anti-ulcerogenic effect and HPLC analysis of the caffeoylquinic acid-rich extract from Ligularia stenocephala. Biol Pharm Bull. 33: 493-497, 2010. https://doi.org/10.1248/bpb.33.493
  26. Terano, A., Hiraishi, H., Ota, S., Shiga, J., Sugimoto, T. Role of superoxide and hydroxyl radicals in rat gastric mucosal injury induced by ethanol. Gastroenterol Jpn. 24: 488-493, 1989.
  27. Loguercio, C., Taranto, D., Beneduce, F., del Vecchio Blanco, C., de Vincentiis, A., Nardi, G., Romano, M. Glutathione prevents ethanol induced gastric mucosal damage and depletion of sulfhydryl compounds in humans. Gut. 34: 161-165, 1993. https://doi.org/10.1136/gut.34.2.161
  28. Saravanan, R., Rajendra Prasad, N., Pugalendi, K.V. Effect of Piper betle leaf extract on alcoholic toxicity in the rat brain. J Med Food. 6: 261-265, 2003. https://doi.org/10.1089/10966200360716689
  29. Schinella, G.R., Tournier, H.A., Prieto, J.M.,. Mordujovich de Buschiazzo, P., Rios, J.L. Antioxidant activity of anti-inflammatory plant extracts. Life Sci. 70: 1023-1033, 2002. https://doi.org/10.1016/S0024-3205(01)01482-5
  30. Whittle, B.J. Thirteenth Gaddum Memorial Lecture. Neuronal and endothelium-derived mediators in the modulation of the gastric microcirculation: integrity in the balance. Br J Pharmacol. 110: 3-17, 1993. https://doi.org/10.1111/j.1476-5381.1993.tb13763.x
  31. Kawano, S., Tsuji, S. Role of mucosal blood flow: a conceptional review in gastric mucosal injury and protection. J Gastroenterol Hepatol. 15: D1-6, 2000.