Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Nitric Oxide Production Inhibitory Effect and Antibacterial Activity of the Extract and Fractions from Paeoniae Radix

작약 메탄올 추출물 및 분획물의 Nitric Oxide 생성 억제 효과와 피부질환 원인균에 대한 항균활성

  • Im, Do-Youn (Dept. of Beauty Science, Kwangju Women's University) ;
  • Lee, Kyoung-In (Biotechnology Industrialization Center, Dongshin University)
  • 임도연 (광주여자대학교 미용과학과) ;
  • 이경인 (동신대학교 생물자원산업화지원센터)
  • Received : 2012.03.08
  • Accepted : 2012.05.02
  • Published : 2012.06.30
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Abstract

In this study, we investigated antibacterial activity and nitric oxide production inhibitory effect of the methanol extract and its fractions from Paeoniae Radix. In antibacterial activity by the disc diffusion assay against S. aureus, S. epidermidis and P. aeruginosa, the ethyl acetate fraction showed stronger antibacterial activity than other fractions and the extract. Moreover, the ethyl acetate fraction showed strong nitric oxide (NO) production inhibitory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 cell. However, in NO scavenging ability, the chloroform fraction was higher than the other fractions and the extract. In the lactate dehydrogenase (LDH) assay against RAW 264.7 cell, the extract and fractions were exhibited normal LDH release level as nontoxic result without the ehtyl acetate fraction of 100 ${\mu}g/ml$. As a result, the ethyl acetate fraction and chloroform fraction of the methanol extract from Paeoniae Radix could be applicable to functional materials for antibacterial and anti-inflammatory related fields, respectively.

Keywords

References

  1. Lee, M. S. (2009) New antimicrobials on the horizon. Kor. J. Medicine 77: 35-51.
  2. Song, J. H. (2009) Current status and future strategies of antimicrobial resistance. Kor. J. Medicine 77: 143-151.
  3. Lee, K. I., Choi, C. H., Kim, S. M. and Pyo, B. S. (2010) Antibacterial activity and Enhancing antibiotic effect of extract and fractions from Curcuma longa against MRSA strain. Kor. J. Pharmacogn. 41: 38-42.
  4. Lee K. I., Yang, S. A., Pyo, B. S. and Kim, S. M. (2011) Antibacterial activity against pathogens of acne and tyrosinase inhibitory activity of extract and fractions from bark of Prunus sargentii. Kor. J. Pharmacogn.. 42: 155-160.
  5. Im, D. Y. and Lee, K. I. (2011) Antioxidative and antibacterial activity and tyrosinase inhibitory activity of the extract and fractions from Taraxacum coreanum Nakai. Korean J. Medicinal Crop Sci. 19: 238-245. https://doi.org/10.7783/KJMCS.2011.19.4.238
  6. Chung, H. T., Pae, H. O., Choi, B. M., Billiar, T. R. and Kim, Y. M. (2001) Nitric oxide as a bioregulator of apoptosis. Biochem. Biophys. Res. Commun. 282: 1075-1079. https://doi.org/10.1006/bbrc.2001.4670
  7. Radi, R., Beckman, J. S., Bush, K. M. and Freeman, B. A. (1991) Peroxynitrite oxidation of sulfhydryls, the cytotoxic potential of superoxide and nitric oxide. J. Biol. Chem. 266: 4244-4250.
  8. Haenen, G. R., Paquay, J. B., Korthhouwer, R. E. and Bast, A. (1997) Peroxinitrite scavenging by flavonoids. Biochem. Biophys. Res. Commun. 236: 591-593. https://doi.org/10.1006/bbrc.1997.7016
  9. Byun, S. H., Yang, C. H. and Kim, S. C. (2005) Inhibitory effect of Scrophulariae Radix extract on TNF-${\alpha}$, IL-$1{\beta}$, IL-6 and nitric oxide production in lipopolysaccharide activated Raw 264.7 cells. Kor. J. Herbology 20: 7-16.
  10. Lee, K. I., Yang, S. A. and Kim, S. M. (2011) Antioxidative and nitric oxide production inhibitory activities of Lespedeza bicolor stem extracts depending on solvents. Korean J. Medicinal Crop Sci. 19: 368-372. https://doi.org/10.7783/KJMCS.2011.19.5.368
  11. Hwang, E. Y., Kim, D. H., Kim, H. J., Hwang, J. Y., Park, T. S., Lee, I. S. and Son, J. H. (2011) Antioxodant activities and nitric oxide production of medicine plants in Gyeongsangbukdo (Carthamus tinctorius seed, Cyperus rotundus, Schizonepeta tenuifolia, Polygonatum odoratum var. pluroflorum, Paeonia lactiflora). J. Appl. Biol. Chem. 54: 171-177. https://doi.org/10.3839/jabc.2011.029
  12. Lee, K. I. and Im, D. Y. (2011) Biological activitirs of the water extract and its fractions from Taraxacum coreanum Nakai. Kor. J. Pharmacogn. 42: 195-200.
  13. Lee, S. J., Lee, I. S. and Mar, M. (2003) Inhibition of inducible nitric oxide synthase and cyclooxygenase-2 activity by 1,2,3,4,6-penta-O-galloyl-beta-D-glucose in murine macrophage cells. Arch. Pharm. Res. 26: 832-839. https://doi.org/10.1007/BF02980029
  14. Yang, H. O., Ko, W. K., Kim, J. Y. and Ko, H. S. (2004) Paeoniflorin: an antihyperlipidemic agent from Paeonia lactiflora. Fitoterapia 75: 45-49. https://doi.org/10.1016/j.fitote.2003.08.016
  15. Yamahara, J., Yamada, T., Kimura, H., Sawada, T. and Fujimura, H. (1982) Biological active principles of crude drugs. II. Anti-allergic principles in "Shoseiryu-To" antiinflammatory properties of paeoniflorin and its derivatives. J. Pharmaco-Dynamics 5: 921-929. https://doi.org/10.1248/bpb1978.5.921
  16. Wei, W.-S., Wang, Y.-Q. and Wang, S.-Y. (1987) Immunoregulatory effects of total glucosides of paeony root in mice. Chin. Pharmacol. Bull. 3: 148-152.
  17. Park, K. D. and Cho, S. H. (2010) Antimicrobial characteristics of Paeonia lactiflora Pall. extract tested against foodputrefactive microorganisms. Korean J. Food Preserv. 17: 706-711.
  18. Im, D. Y. and Lee, K. I. (2011) Tyrosinase inhibitory activity and melanin production inhibitory activity of the extract and fractions from Paeoniae Radix. Kor. J. Pharmacogn. 42: 323-328.
  19. Marcocci, L., Maguire, J. J., Droy-Lefaix, M. T. and Packer, L. (1994) The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochem. Biophys. Res. Commun. 201: 748-755. https://doi.org/10.1006/bbrc.1994.1764
  20. Ding, A. H., Nathan, C. F. and Stuehr, D. J. (1988) Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J. Immunol. 141: 2407-2412.
  21. Lee, K. I., Kim, S. M., Kim, S. M. and Pyo, B. S. (2011) Comparison of fatty acids and antibacterial activity against pathogen of acne in different parts of ripened black raspberry( Rubus coreanus Miquel). J. Korean Soc. Food Sci. Nutr. 40: 466-469. https://doi.org/10.3746/jkfn.2011.40.3.466
  22. Park, K. D. and Cho, S. H. (2010) Antimicrobial characteristics of Paeonia lactiflora Pall. extract tested against foodputrefactive microorganisms. Korean J. Food Preserv. 17: 706-711.
  23. Choi, H. Y. (2009) Antimicrobial activity of Paeonia japonica extract and its quality characteristic effects in Sulgidduk. Korean J. Food Cookery Sci. 25: 435-444.
  24. Hwang, J. S., Chun, H. J. and Han, Y. S. (2000) Isolation and identification of antimicrobial compound from Jakyak(Paeonia japonica var. pilosa Nakai). Korean J. Soc. Food Sci. 16: 445-452.
  25. Choi, H. S., Kim, Y. C., Lee, J. S., Jo, M. R., Seo, C. H. and Park, S. I. (2004) Antibacterial activities of hot-water and ethyl alcohol extracts of medicinal herbs on fish pathogenic bacteria. J. Fish Pathol. 17: 39-55.
  26. Chung, H. T., Pae, H. O., Choi, B. M., Billiar, T. R. and Kim, Y. M. (2001) Nitric oxide as a bioregulator of apoptosis. Biochem. Biophys. Res. Commun. 282: 1075-1079. https://doi.org/10.1006/bbrc.2001.4670
  27. Lee, E. S., Ju, H. K., Moon, T. C., Lee, E., Jang, Y., Lee, S. H., Son, J. K., Baek, S. H. and Chang, H. W. (2004) Inhibition of nitric oxide and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) production by propenone compound through blockade of nuclear factor(NF)-${\kappa}B$ activation in cultured murine macrophage. Biol. Pharm. Bull. 27: 617-620. https://doi.org/10.1248/bpb.27.617
  28. Kang, T. B. and Yoon, T. J. (2011) Effect of herbal composition on alcohol degradation and anti-inflammatory activity in mice. Korean J. Food & Nutr. 24: 489-495. https://doi.org/10.9799/ksfan.2011.24.4.489
  29. Ko, S. K. and Pyo, M. Y. (2011) Anti-inflammatory effect of Inonotus obliquus extracts in lipopolysaccharide-induced mouse peritoneal macrophage. Kor. J. Pharmacogn. 42: 253-259.
  30. Lee, J. Y., Ko, S. H., Lee, Y. J., Lee, S. Y., Park, H. J., Shin, T. Y. and Jeon, H. (2010) Anti-inflammatory effect of MeOH extract of Cibotium barometz in IFN- and LPS-stimulated mouse peritoneal macrophage. Kor. J. Pharmacogn. 41: 108-114.
  31. Fotakis, G. and Tmbrell, A. J. (2006) In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicology Letters 160: 171-177. https://doi.org/10.1016/j.toxlet.2005.07.001