Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Genetic Variation in Wild and Cultured Populations of the Sea Squirt Halocynthia roretzi Inferred from Microsatellite DNA Analysis

  • Han, Hyon-Sob (West-Sea Fisheries Research Institute, National Fisheries Research and Development Institute (NFRDI)) ;
  • Nam, Bo-Hye (Biotechnology Research Center, NFRDI) ;
  • Kang, Jung-Ha (Biotechnology Research Center, NFRDI) ;
  • Kim, Yi-Kyoung (Biotechnology Research Center, NFRDI) ;
  • Jee, Young-Ju (Biotechnology Research Center, NFRDI) ;
  • Hur, Young-Baek (Aquaculture Environmental Institute, NFRDI) ;
  • Yoon, Moon-Geun (Yangyang Salmon Station, Korea Fisheries Resources Agency)
  • Received : 2012.02.06
  • Accepted : 2012.03.20
  • Published : 2012.06.30
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Abstract

We used nine microsatellite DNA markers to estimate genetic variation among wild and cultured populations of the sea squirt Halocynthia roretzi. The loci were polymorphic, with 6-32 alleles, and allelic richness ranged from 6.0 to 26.1 in each population. The wild and the cultured populations had similar mean heterozygosities ($H_O$ and $H_E$), allele numbers, and allelic richness. One cultured population with softness syndrome had a lower mean in the observed heterozygosity ($H_O$ = 0.57) and higher mean inbreeding coefficient ($F_{IS}$ = 0.261) than any other populations. This suggests that the loss of genetic variation in the diseased population might be due to increased inbreeding. A neighbor-joining tree and pairwise population estimates of $F_{ST}$ showed moderate genetic differentiation between the wild and the cultured populations. Additionally, the softness syndrome population was genetically divergent from wild populations, but it was genetically close to the cultured populations.

Keywords

References

  1. Allendorf FW and Phelps SR. 1980. Loss of genetic variation in a hatchery stock of cutthroat trout. Trans Am Fish Soc 109, 537-543. https://doi.org/10.1577/1548-8659(1980)109<537:LOGVIA>2.0.CO;2
  2. Allendorf FW and Ryman N. 1987. Genetic management of hatchery stocks. In: Population Genetics and Fishery Management. Ryman N and Utter F, eds. University of Washington Press, Seattle, WA, US, pp. 141-159.
  3. Ben-Shlomo R, Douek J and Rinkevich B. 2001. Heterozygote deficiency and chimerism in remote populations of a colonial ascidian from New Zealand. Mar Ecol Prog Ser 209, 109-117. https://doi.org/10.3354/meps209109
  4. Ben-Shlomo R, Paz G and Rinkevich B. 2006. Postglacial-period and recent invasions shape the population genetics of Botryllid ascidians along European Atlantic Coasts. Ecosystems 9, 1118-1127. https://doi.org/10.1007/s10021-006-0141-y
  5. Cho HK, Nam BH, Kong HJ, Han HS, Hur YB, Choi TJ, Choi YH, Kim WJ and Cheong J. 2008. Identification of softness syndrome-associated candidate genes and DNA sequence variation in the sea squirt, Halocynthia roretzi. Mar Biotechnol 10, 447-456. https://doi.org/10.1007/s10126-008-9084-y
  6. Davis AR and Butler AJ. 1989. Direct observations of larval dispersal in the colonial ascidian (Podoclavella moluccensis Sluiter): evidence for closed populations. J Exp Mar Biol Ecol 127, 189-203. https://doi.org/10.1016/0022-0981(89)90184-6
  7. Felsenstein J. 1993. PHYLIP (Phylogeny Interference Package) version 3.5c. Department of Genetics, Washington State University, Seattle, WA, US.
  8. Goudet J. 2001. FSTAT, a program to estimate and test gene diversities and fixation indices (version 2. 9. 3) [Internet]. Universite de Lausanne, Suisse, CH, Accessed 1 Mar 201 2, http://www.unil.ch/izea/softwares/fstat.html .
  9. Grosberg RK. 1987. Limited dispersal and proximity-dependent mating success in the colonial ascidian Botryllus schlosseri. Evolution 41, 372-384. https://doi.org/10.2307/2409145
  10. Han HS, Nam BH, Kang JH, Choi TJ, Kim WJ, Hur YB and Yoon M. 2009. Isolation and characterization of polymorphic microsatellite DNA markers in the sea squirt, Halocynthia roretzi. Mol Ecol Resour 9, 648-650. https://doi.org/10.1111/j.1755-0998.2008.02499.x
  11. Hurt C and Hedrick P. 2004. Conservation genetics in aquatic species: general approaches and case studies in fishes and springsnails of arid lands. Aquat Sci 66, 402-413. https://doi.org/10.1007/s00027-004-0726-5
  12. Kang PA, Kim Y and Yoon DS. 1980. Studies on the hanging culture of oyster, Crassostrea gigas, in the Korean coastal waters. 4. On the fouling organisms associated with culturing oysters at the oyster culture farms in Chungmu. Bull Fish Res Dev Agency Busan 25, 29-34.
  13. Lopez-Legentil S and Turon X. 2006. Population genetics, phylogeography and speciation of Cystodytes (Ascidiacea) in the western Mediterranean Sea. Biol J Linn Soc 88, 203-214. https://doi.org/10.1111/j.1095-8312.2006.00614.x
  14. Ministry of Maritime Affairs and Fisheries. 2010. Statistical Database for Fisheries Production [Internet]. Korea National Statistical Office, Daejeon, KR, Accessed 6 Feb 2011, http://fs.fips.go.kr/main.jsp.
  15. Nei M. 1972. Genetic distance between populations. Am Nat 106, 283-292. https://doi.org/10.1086/282771
  16. Rho YG, Lee YH and Park MW. 1993. The environmental factors affecting mortality of cultured sea squirt, Halocynthia roretzi (Drasche). Bull Nat'l Fish Res Dev Agency (Korea) (47), 145-164.
  17. Rousset F and Raymond M. 1995. Testing heterozygote excess and deficiency. Genetics 140, 1413-1419.
  18. Slatkin M. 1995. A measure of population subdivision based on microsatellite allele frequencies. Genetics 139, 457-462.
  19. Tarjuelo I, Posada D, Crandall A, Pascual M and Turon X. 2004. Phylogeography and speciation of colour morphs in the colonial ascidian Pseudodistoma crucigaster. Mol Ecol 13, 3125-3136. https://doi.org/10.1111/j.1365-294X.2004.02306.x
  20. Weir BS and Cockerham CC. 1984. Estimating F-statistics for analysis of population structure. Evolution 38, 1358-1370. https://doi.org/10.2307/2408641

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