Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Proteome Analysis of Bovine Longissimus dorsi Muscle Associated with the Marbling Score

  • Shen, Y.N. (Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences) ;
  • Kim, S.H. (Department of Biology, Kyung Hee University) ;
  • Yoon, D.H. (Department of Animal Science, Kyungpook National University) ;
  • Lee, H.G. (Department of Bio-Resources, Natural Resource and Life Science, Busan National University) ;
  • Kang, H.S. (Department of Animal Science and Technology, Sunchon National University) ;
  • Seo, K.S. (Department of Animal Science and Technology, Sunchon National University)
  • Received : 2012.03.07
  • Accepted : 2012.04.24
  • Published : 2012.08.01
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Abstract

The breeding value of marbling score in skeletal muscle is an important factor for evaluating beef quality. In the present study, we investigated proteins associated with the breeding value of the marbling score for bovine sirloin to select potential biomarkers to improve meat quality through comparative proteomic analysis. Proteins isolated from muscle were separated by two-dimensional gel electrophoresis. After analyzing images of the stained gel, seven protein spots for the high marbling score group were identified corresponding to changes in expression that were at least two-fold compared to the low marbling score group. Four spots with increased intensities in the high marbling score group were identified as phosphoglycerate kinase 1, triosephophate isomerase, acidic ribosomal phosphoprotein PO, and capping protein (actin filament) Z-line alpha 2. Spots with decreased intensities in the high marbling score group compared to the low score group were identified as 14-3-3 epsilon, carbonic anhydrase II, and myosin light chain 1. Expression of myosin light chain 1 and carbonic anhydrase 2 was confirmed by Western blotting. Taken together, these data could help improve the economic performance of cattle and provide useful information about the underlying the function of bovine skeletal muscle.

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