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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Proteome analysis of developing mice diastema region

  • Chae, Young-Mi (Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University) ;
  • Jin, Young-Joo (Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University) ;
  • Kim, Hyeng-Soo (School of Science and Biotechnology, Kyungpook National University) ;
  • Gwon, Gi-Jeong (Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University) ;
  • Sohn, Wern-Joo (Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University) ;
  • Kim, Sung-Hyun (Department of Center for Laboratory Animal Resources, Kyungpook National University) ;
  • Kim, Myoung-Ok (Department of Animal Science, Kyungpook National University) ;
  • Lee, Sang-Gyu (School of Science and Biotechnology, Kyungpook National University) ;
  • Suh, Jo-Young (Department of Periodontology, Kyungpook National University) ;
  • Kim, Jae-Young (Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University)
  • Received : 2012.02.01
  • Accepted : 2012.02.11
  • Published : 2012.06.30
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Abstract

Different from humans, who have a continuous dentition of teeth, mice have only three molars and one incisor separated by a toothless region called the diastema in the hemi mandibular arch. Although tooth buds form in the embryonic diastema, they regress and do not develop into teeth. In this study, we evaluated the proteins that modulate the diastema formation through comparative analysis with molar-forming tissue by liquid chromatography-tandem mass spectroscopy (LC-MS/MS) proteome analysis. From the comparative and semi-quantitative proteome analysis, we identified 147 up- and 173 down-regulated proteins in the diastema compared to the molar forming proteins. Based on this proteome analysis, we selected and evaluated two candidate proteins, EMERIN and RAB7A, as diastema tissue specific markers. This study provides the first list of proteins that were detected in the mouse embryonic diastema region, which will be useful to understand the mechanisms of tooth development.

Keywords

References

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