Biomolecules & Therapeutics

  • 제20권3호
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  • Pages.320-325
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  • 2012
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  • 1976-9148(pISSN)
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  • 2005-4483(eISSN)
한국응용약물학회 (The Korean Society of Applied Pharmacology)
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Expression of Recombinant Rotavirus Proteins Harboring Antigenic Epitopes of the Hepatitis A Virus Polyprotein in Insect Cells

  • Than, Van Thai (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Baek, In-Hyuk (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Lee, Hee-Young (Medikan Inc.) ;
  • Kim, Jong-Bum (Functional Biomaterial Division, National Academy of Agricultural Science, the Rural Development Administration) ;
  • Shon, Dong-Hwa (Food Immunology Research Group, Korea Food Research Institute) ;
  • Chung, In-Sik (Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Won-Yong (Department of Microbiology, Chung-Ang University College of Medicine)
  • 투고 : 2011.12.21
  • 심사 : 2012.03.20
  • 발행 : 2012.05.31
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초록

Rotavirus and hepatitis A virus (HAV) spread by the fecal-oral route and infections are important in public health, especially in developing countries. Here, two antigenic epitopes of the HAV polyprotein, domain 2 (D2) and domain 3 (D3), were recombined with rotavirus VP7, generating D2/VP7 and D3/VP7, cloned in a baculovirus expression system, and expressed in Spodoptera frugiperda 9 (Sf9) insect cells. All were highly expressed, with peak expression 2 days post-infection. Western blotting and ELISA revealed that two chimeric proteins were antigenic, but only D2/VP7 was immunogenic and elicited neutralizing antibody responses against rotavirus and HAV by neutralization assay, implicating D2/VP7 as a multivalent subunit-vaccine Candidate for preventing both rotavirus and HAV infections.

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피인용 문헌

  1. Expression and immunogenic analysis of recombinant polypeptides derived from capsid protein VP1 for developing subunit vaccine material against hepatitis A virus vol.100, 2014, https://doi.org/10.1016/j.pep.2014.04.011
  2. Immunological properties of the chicken egg yolk (IgY) antibodies against Vietnamese cobra Naja Naja venom vol.991, pp.None, 2012, https://doi.org/10.1088/1757-899x/991/1/012012