DOI QR코드

DOI QR Code

Antioxidant and Antimicrobial Activities of Sorghum Germplasms Introduced from USA

미국 도입 수수 유전자원의 항산화활성과 항균활성

  • Goh, Eun-Jeong (Advenced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Yoo, Ji-Hye (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University) ;
  • Seong, Eun-Soo (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University) ;
  • Lee, Jae-Geun (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University) ;
  • Hwang, In-Seong (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University) ;
  • Kim, Nam-Jun (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University) ;
  • Yu, Chang-Yeon (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University)
  • 고은정 (한국원자력연구원 정읍방사선과학연구소 방사선육종연구팀) ;
  • 유지혜 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 성은수 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 이재근 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 황인성 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 김남준 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 유창연 (강원대학교 농업생명과학대학 식물자원응용공학과)
  • Received : 2011.09.22
  • Accepted : 2012.03.21
  • Published : 2012.04.30

Abstract

This study was carried out to evaluate the agronomic characteristics and biological activities of $Sorghum$ $bicolor$ germplasms introduced from USA. Plant height, stem diameter, tiller number, leaf length, leaf width, leaf vein color, ear type, ear length and ear width have different from the cultivated accessions. Sweet-N-Sterile (#4) showed the tallest height, widest ear-width and stem diameter. Most of $Sorghum$ $bicolor$ plants may be available to use for bio-energy from these results showing big biomass. Antioxidant activities of 11 cultivars collected from USA were examined by DPPH assay and reducing power. Among the cultivars, Premium stock (#1), Early Sumac (#7), SS Silage (#9) and WGF Grain Sorghum (#11) showed a significantly higher antioxidant activity in comparison to others. Early Sumac (#7) and SS silage (#9) showed more strong reducing power activities than ${\alpha}$-tocopherol, a positive control. Premium stock (#1), Sweet-N-Sterile (#4), Early Sumac (#7) and SS Silage (#9) were also showed high antioxidant activities by DPPH assay and reducing power experiment. BMR Gold I (#3) displayed strong antimicrobial activity against $Escherichia$ $coli$ at minimum inhibitory concentrations (125 ${\mu}g$/ml).

본 연구는 미국 수수 수집종의 작물학적 특성 및 생리활성을 검정하기 위하여 수행되었다. 대부분의 미국 수수 수집종은 200 cm 이상의 큰 초장을 보였으며, 간경 또한 2cm 이상의 값을 나타냈다. 수수 수집종 중에서는 Sweet-N-Sterile(#4)이 가장 우량한 수수의 생장 특성을 보였다. 수수의 DPPH를 이용한 활성 검정 결과, Premium stock(#1), Early Sumac(#7), SS Silage(#9), WGF Grain Sorghum(#11) 4개의 수집종에서 기존의 항산화제인 ${\alpha}$-tocopherol과 BHA와 비슷한 활성을 보였고 BHT보다는 높은 활성을 보였다. 항미생물 실험결과 BMR Gold I(#3)이 가장 높은 활성을 나타냈으며, 특히 $Escherichia$ $coli$에서 125 ${\mu}g$/ml으로 높은 활성수치를 나타냈다.

Keywords

References

  1. Awika, J.M., L.W. Rooney, X. Wu, R.L. Prior and L. Cisneros-Zevallos. 2004. Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. J. Agr. Food Chem. 51:6657-6662.
  2. Cho, N.K., Y.K. Kang, C.K. Song, Y.C. Jeun, J.S. Oh, Y.I. Cho and S.J. Park. 2004. Effects of planting density on growth, forage yield and chemical composition of Jeju native Sorghum (Sorghum bicolor L.). J. Korean Grassl. Sci. 24:225-230 (in Korean). https://doi.org/10.5333/KGFS.2004.24.3.225
  3. Goh, E.J., E.S. Seong, J.H. Yoo, H.Y. Kil, J.G. Lee, I.S. Hwang, N.J. Kim, B.K. Ghimire, M.J. Kim, J.K. Lee, J.D. Lim, N.Y. Kim and C.Y. Yu. 2011. Effect of plant growth regulators and media on regeneration of sorghum bicolor (L.) Moench. J. Korean Plant Res. 24(2):168-173 (in Korean). https://doi.org/10.7732/kjpr.2011.24.2.168
  4. Hilal, M., M.F. Parrado, M. Rosa, M. Gallardo, L. Orce, E.M. Massa, J.A. Gonzalez and F.E. Prado. 2004. Epidermal lignin deposition in quinoa cotyledons in response to UV-B radiations. Photochem. Photobiol. 79(2):205-210. https://doi.org/10.1562/0031-8655(2004)079<0205:ELDIQC>2.0.CO;2
  5. Kil, H.Y., E.S. Seong, B.K. Ghimire, I.M. Chung, S.S. Kwon, E.J. Goh, K. Heo, M.J. Kim, J.D. Lim, D. Lee and C.Y. Yu. 2009. Antioxidant and antimicrobial activities of crude sorghum extract. Food Chem. 115:1234-1239. https://doi.org/10.1016/j.foodchem.2009.01.032
  6. Kwak, C.S., S.J. Lim, S.A. Kim, S.C. Park and M.S. Lee. 2004. Antioxidative and antimutagenic effects of Korean Buckwheat, Sorghum, Millet and Job's Tears. J. Korean Soc. Food Sci. Nutr. 33(6):921-929. https://doi.org/10.3746/jkfn.2004.33.6.921
  7. Lee, J.H. and S.R. Lee. 1994. Some physiological activity of phenolic substances in plant foods. Korean J. Food Sci. Technol. 26(3):317-323.
  8. Lee, K.E., J.Y. Lee and K. Kim. 2008. Effect of content of crop component on the bioethanol production. Korean J. Crop Sci. 53(3):339-346 (in Korean).
  9. Ma, Q.H. and Y. Xu. 2008. Characterization of a caffeic acid 3-O-methyltransferase from wheat and its function in lignin biosynthesis. Biochimie 90:515-524. https://doi.org/10.1016/j.biochi.2007.09.016
  10. Oyaizu M. 1986. Studies on products of browning reactions: andtioxidative activities of products of browning reaction prepared from glucosamine. Japanese J. Nutr. 44:307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  11. Prasad, K.N., H. Xie, J. Hao, B. Yang, S. Qiu, X. Wei, F. Chen and Y. Jiang. 2010. Antioxidant and anticancer activities of 8-hydroxypsoralen isolated from wampee [Clausena lansium (Lour.) Skeels] peel. Food Chem. 118: 62-66. https://doi.org/10.1016/j.foodchem.2009.04.073
  12. Ryu, H.S., J. Kim and H.S. Kim. 2006. Enhancing effect of sorghum bicolor L. Moench (sorghum, su-su) extracts on mouse spleen and macrophage cell activation. J. Korean Diet. Assoc. 19:176-182.
  13. Seong, E.S., J.H. Yoo, J.G. Lee, I.S. Hwang, N.J. Kim, M.J. Kim, J.K. Lee, B.K. Ghimire, J.D. Lim and C.Y. Yu. 2011. Agronomic characterization of Sorghum bicolor (L) Moench germplasm collected in Gangwon-do for bioenergy crop. Korean J. Crop Sci. 56(2):151-158 (in Korean). https://doi.org/10.7740/kjcs.2011.56.2.151
  14. Xiong, Q., S. Kadota, T. Tani and T. Namba. 1996. Antioxidative effect of phenylethanoids from Cistanche deserticola. Biol. Pharm. Bull. 19(12):1580-1585. https://doi.org/10.1248/bpb.19.1580
  15. Zhao, Z., C. Tishu, T. Laura, M. Mike, W. Ning, P. Hong, R. Marjorie, S. Sheryl, H. Dave, S. Jon and P. Dortie. 2000. Agrobacterium-mediated sorghum transformation. Plant Mol. Biol. 44:789-798. https://doi.org/10.1023/A:1026507517182

Cited by

  1. Evaluation of Crop Characteristics of Sorghum (Sorghum bicolor L.) Germplasm for the Selection of Excellent Resources vol.29, pp.4, 2016, https://doi.org/10.7732/kjpr.2016.29.4.479
  2. 수수 수집종의 농업적 형질 및 생리활성 분석을 통한 우수계통 선발 vol.24, pp.5, 2016, https://doi.org/10.7783/kjmcs.2016.24.5.386